| Project/Area Number |
03045045
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | University-to-University Cooperative Research |
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| Research Institution | Tokai University |
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Principal Investigator |
WATANAVE Keiichi Tokai University School of Medicine, 医学部, 教授 (00055865)
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| Co-Investigator(Kenkyū-buntansha) |
NABER Stephan p. Tufts Univ., School of Medicine, Sch. of Med. ・Dept. of Pathol., Assistant
WOLFE Hubert j. Tufts Univ., School of Medicine, Sch. of Med. ・Dept. of Pathol., Professor
TSUTSUMI Yutaka Tokai University School of Medicine, 医学部, 助教授 (80138643)
MORIUCHI Tetsuya Tokai University School of Medicine, 医学部, 助教授 (20174394)
STEPHAN P.Na Tufts Univ., Sch.of Med.・Dept.of Pathol., Assistant
HUBERT J.Wol Tufts Univ., Sch.of Med.・Dept.of Pathol., Professor
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| Project Period (FY) |
1991 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥5,200,000)
Fiscal Year 1993: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1992: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1991: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | p53 protein / colonic cancer in adenoma / point mutation / in situ PCR technique / immunohistochemistry / antigen retrieval / homeo box gene / Hox4B protein / P^<53>蛋白 / 抗源性賦活化 / Hox4B / tumor banking system / 胃癌 / 乳癌 / polymerase chain reaction / 癌抑制遺伝子 / P^<53> / heat shock protein |
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| Research Abstract |
1. Antigen retrieval method for p53 protein in formalin-fixed, paraffin-embedded sections were established. Nuclear localization of p53 protein in adenocarcinoma cells was reproducibly observed by treating deparaffinized sections with 90ﾟC heating for 120 min in PBS. Normal cells remained completely negative. Dysplastic cells in adenomas also expressed p53 protein, but it was mainly seen after antigen retrieval. The degree of p53 antigen retrieval was dependent upon the degree of cellular dysplasia, suggesting the possibility that the mutant p53 protein forms complexes with the wild-type protein in adenoma cells.
2. Technical aspects of in situ PCR was presented and demonstrated by Prof.Wolfe either at the seminar in Tokai University or in the lab of Tufts University. This technique is particularly useful in high-sensitivity detection of the genome of pathogens such as HIV and EBV.
3. Polyclonal and monoclonal antibodies against the Hox4B protein encoded by a homeo box gene were established. By immunohistochemical screening, Hox4B protein was expressed in normal and neoplastic human adrenal medulla. Expression of the Hox4B protein was also seen in PCNA-negative cells in rat fetus, suggesting that its expression is closely related to the programmed cell death.
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