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生殖系列の分子機構に関する基礎研究

Research Project

Project/Area Number 03207102
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Research InstitutionThe Open University of Japan

Principal Investigator

毛利 秀雄  放送大学, 教養学部, 教授 (70012268)

Co-Investigator(Kenkyū-buntansha) 舘 鄰  東京大学, 農学系研究科, 助教授 (30011711)
高橋 迪雄  東京大学, 農学部, 教授 (30011943)
入谷 明  京都大学, 農学部, 教授 (80026385)
森 崇英  京都大学, 医学部, 教授 (90026865)
星 元紀  東京工業大学, 生命理工学部, 教授 (20012411)
Project Period (FY) 1988 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥11,000,000 (Direct Cost: ¥11,000,000)
Fiscal Year 1991: ¥11,000,000 (Direct Cost: ¥11,000,000)
Keywords生殖系列 / 配偶子 / 受精 / 着床 / 胎盤形成 / 初期胚 / トランスジェニック動物
Research Abstract

本重点領域研究では、生殖系列の分子機構に関する基礎研究を発展させることを目的として、配偶子分化の分子機構・受精の分子機構・着床と胎盤形成の分子機構・初期胚分割の分子機構の4主要項目について、49名の分担研究者による研究課題を設け、活発な研究活動を展開している。本研究班(総括班)は、研究者間の情報交換の場を提供し、研究費の効率的な運用と活発な研究活動の維持・促進のために、以下の様な活動を行なった。
(1)班長会議:研究代表者が招集し、班長および事務担当者で構成する。本年度は、4・9・11月の計3回開催し、研究計画の立案、研究経費の配分と効率的運用などについて協議した。
(2)研究連絡会の開催:計画・公募研究者全員が参加するもので、2名以上の演者による学術講演と、それに関する討論を行なう一方、班員相互の連絡の場となっている。本年度は、4・9・11月の計4回開催し、本重点領域研究発足年度(昭和63年度)より通算して、計17回の研究連絡会を開催した。
(3)ニュ-スレタ-:本年度は、7・9・2月の3回発行し、研究連絡会学術講演論文や、事務局からの連絡などを掲載した。また、これ以外に増刊号を2回(第2回シンポジウムプロシ-デイング号、第3回シンポジウム抄録集)発行した。
(4)シンポジウム:本年度は、平成4年1月17日に、京都で開催した。生殖系列研究において、特に著しい成果を挙げた5人(2名の招待講演者(うち1人は外国人研究者)、3名の本重点領域研究班員)に講演を依頼し、最新情報・技術に関する活発な討論が行なわれた。
(5)平成3年度が本重点領域の最終年度であることに鑑み、「最終年度研究成果取り纒め報告書」のための準備委員会を設置し、当該報告書の作成準備を開始した。また、本重点領域研究の研究成果を一般に公開することを目的として、「研究成果出版」準備委員会組織し、活動を開始した。

Report

(1 results)
  • 1991 Annual Research Report
  • Research Products

    (93 results)

All Other

All Publications (93 results)

  • [Publications] Oshio,S.: "Effects of mecobalamin on oligozoospermia induced by feeding a vitamin B_<12> deficient diet in nice." Japanese Journal of Fertility and Sterility. 36. 178-184 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Wada,S.: "Inner arm dynein ATPase fraction of sea urchin sperm flagella causes active sliding of axonemal outer doublet microtubule." Biochemical and Biophysical Research Communications. 175. 173-178 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Oshio,S.: "Effects of mecobalamin on tesictular dysfunction induced by xーray irradiation in mice." Folia Pharmacol.Japon.98. 483-490 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kobayashi,T.: "Human acrosin:Purification and some properties." Archives of Andrology. 27. 9-16 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Inaba,K.: "Mapping of ATPーdependent trypsinーsensitive sites on the β chain of outerーarm dynein from sea urchin sperm flagella." Journal of Biochemistry. 110. 795-801 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Ishijima,S.A.: "Zata potential of human Xーand Yーbearing sperm." International Journal of Andrology. 14. 340-347 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] T.Amano: "Treatment of Starfish Sperm with Egg Jally Induces the Degraーdation of Histones" Develop.Growth,Differ.34. 99-106 (1992)

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      1991 Annual Research Report
  • [Publications] 星 元紀: "受精における卵ゼリ-サポニンの役割" 化学. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 星 元紀: "ウニ、ヒトデ、ホヤの受精" タンパク質、核酸、酸素 臨時増刊「復合糖質ー細胞認識から病態までー」. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] A.Irie: "A New Series of Gangliosides Having Sialic Acid Linkage to the NーGlycolyl Hydroxyl Group from the Spermatozoa of the Starfish,Asterias amurensis" J.Biochem.(1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] A.Irie: "Immunological Detection of SialylーSialyl Linkage through the NーGlycolyl Hydroxyl Group in Starfish Gangliosides" J.Biochem.(1992)

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      1991 Annual Research Report
  • [Publications] H.Sawada: "Inhibition of Mouse Blastocyst hatch by SubsiteーSpecific Trypーsin Inhibitors,PeptidylーArginnals" J.Biochem.(1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Ishijima,S.A.: "Separation of murine Xーand Yーsperm by free‐flow electrophoresis wes evaluated by PCR." Proceedings of the 6th Annual Meeting of the Japan Society for Immunogy of Reproduction. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Ishijima,S.A.: "Separation of Xー and Yーchromosomeーbearing murine sperm by freeーflow electrophoresis:Evaluation of separation using PCR." Zoological Science. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Ishijima,S.A.: "Identification of Xーand Yーchromosomeーbearing humam sperm separated by freeーFlow electrophoresis using Yーchromosome specific polymerase chain reac" (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] T.Yasuhara: "Involvement of a Sperm Aminopeptidase in Fertilization of the Sea Urchin" Experientia. 47. 100-103 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] H.Tadenuma: "Purification and Characterization of a GTPーBinding Protein Seving as Pertussin Toxin Substrate in Starfish Oocytes" Arch.Biochem.Biophys.290. 411-417 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] E.Sato: "A New Fluorogenic Substrate with Pyranine System for Phosphaーtases" Chem.Pharm.Bull.(1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Iwakura,Y.: "Transgenic mice carrying interferon Genes." Mol.Reprod.Deverop.(1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Hayashi,T.: "RNA packaging signal of human immunodeficiency virus type I." Virology. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Iwakura,Y.: "Induction of inflammatory arthropathy resembling rheumatoid arthritis in mice transgenic for HTLVーI." Science. 253. 1026-1028 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 岩倉 洋一郎: "HTLVーIによる関節炎の誘導ートランスジェニックマウスを用いた病原性の検討ー" 実験医学. 9. 56-60 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 岩倉 洋一郎: "トランスジェニック動物." TRC News. 1. 1-10 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 塩谷 雅英: "管腔構造をとる臟器内(卵管・静脈・尿管)でのマウス初期胚培養" 日本受精着床学会雑誌.

    • Related Report
      1991 Annual Research Report
  • [Publications] Shiotani,M.: "Immunohistochemical localization of superoxide dismutase in the human ovary." Human Reproduction. 6. 1349-1353 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Narimoto,K.: "Role of Superoxide Dismutase in the Fallopian Tubs Function.Immunohistochemical Assessment of Superoxide Dismutase in the Fallopian Tube." Acta Histochemica et cytochemica. 24. 85-91 (1991)

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      1991 Annual Research Report
  • [Publications] Kishi,J.: "Block to development in cultured rat lーcell embryos is overcome using medium HECMーl." Human Reproduction. 6. 1445-1448 (1991)

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      1991 Annual Research Report
  • [Publications] Nonogaki,T.: "protection of mouse embryos fertilized in vitro from oxidative stress by thioredoxin and superoxide dismutase." Human Reproduction. 6. 1305-1310 (1991)

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      1991 Annual Research Report
  • [Publications] Umaoka,Y.: "Developmental potentiality of embryos cultured under low oxygen tension with superoxide dismutase." Journal of in Vitro Fertilization and Embryo Transfer. (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Umaoka,Y.: "Effects of Oxygen Toxicity on Early Davelopment of Mouse Embryos." Molecular Reproduction and Development.31. 28-33 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Noda,Y.: "Embryo development in vitro." Assisted Reproduction Reviews. (1992)

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      1991 Annual Research Report
  • [Publications] Goto,Y.: "Oxidative stress on mouse embryo development in vitro." J.Free Radical Biology & Medicine. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 岸 淳二: ""リン酸がラット初期胚発生に与える影響"" 日本受精着床学会雑誌.

    • Related Report
      1991 Annual Research Report
  • [Publications] 松本 央: ""マウス胚至適培養条件の検討ーBWW,Ham's Fー10およびαーMEMの評価ー"" 日本受精着床学会雑誌.

    • Related Report
      1991 Annual Research Report
  • [Publications] 夏山 知: ""金属イオンキレ-ト物質による初期胚発生促進効果とその意義"" 日本受精着床学会雑誌.

    • Related Report
      1991 Annual Research Report
  • [Publications] 後藤 康夫: ""子宮内膜と初期胚発育"" 日本受精床学会雑誌.

    • Related Report
      1991 Annual Research Report
  • [Publications] 岸 淳二: ""SDDおよび酸素がラット初期胚発生に与える影響の検討"" 日本受精着床学会雑誌. 第8巻. 82-84 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 野田 洋一: ""卵管由来胚発育促進活性の解析"" 日本不妊学会雑誌. 第36巻. 84(326)-91(333) (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 後藤 康夫: ""ハムスタ-1細胞期胚培養による胚盤胞形成"" 哺乳動物卵子学会雑誌. 第8巻. 71-72 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 夏山 知: ""ThioredoxinによるMouse 2ーcell block解除効果"" 哺乳動物卵子学会雑誌. 第8巻. 105-106 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 後藤 康夫: ""マウス初期胚発育に及ぼす酸化的ストレスの評価"" 哺乳動物卵子学会雑誌. 第8巻. 101-102 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 中山 貴弘: ""ハムスタ-体外受精胚の4細胞期胚への発生"" 日本受精着床学会雑誌. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] C.Tachi: "Pissible patterns of differentiation in the primitive ectoderm of C3H/HeN〈-〉BALB/cA chimeric blastocysts:an inference from quantitaーtive analysis of coat color patterns." Develop.Growth & Differ.33. 45-55 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] C.Tachi: "Partial characterization of macromolecular components in fetal bovine serum required for development of mouse blastocysts cultured in vitro" Develop.Growth & Differ.34. 66-76 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Narimoto,K.: "Immunohistochemical Assessment of Superoxide Dismutase Expression in the Human Endometrium Throughout the Menstrual Cycle." Acta Histochememica et Cytochemica. 23. 487-498 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] Natsuyama,S.: "Ultrastructural study of primary culture of the cells from porcine oviductal epithelium." Journal of Clinical Electron Microscopy. 23. 904-905 (1990)

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      1991 Annual Research Report
  • [Publications] Tokura,T.: "Ultrastructural study of mouse embryos:effect of superoxide dismutase" Journal of Clinical Electron Microscopy. 23. 892-893 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] Mori,T.: "Endocrine and Immune Implications of Human Endometrial Decidualization in Implantation." Annal NY Acad.Sci.626. 321-330 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] T.Yasuhara: "Involvement of a Sperm Aminopeptidase in Fertilization of the Sea Urchin" Experientia. 47. 100-103 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] H.Tadenuma: "Purification and Characterization of a GTPーBinding Protein Serving as Pertussin Toxin Substrate in Starfish Oocytes" Arch.Biochem.Biophys. 290. 411-417 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] E.Sato: "A New Fluorogenic Substrate with Pyranine System for Phosphatases" Chem.Pharm.Bull.(1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] T.Amano: "Treatment of Starfish Sperm with Egg Jelly Induces the Degradation of Histones" Develop.Growth,Differ.34. 99-106 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] M.Takagi: "Participation of 650ーKDa Protease(20S Proteasome)in Starfish Oocyte Maturation" Develop.Biol.150. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] M.Ogiso: "Senile CataractーRelated Accumulation of Lewis^x Glycolipid in Human Lens" J.Biol.Chem.(1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 井田 憲司: ""マウス胚における接着能獲得時期の検討"" 日本受精着床学会雑誌. 第8巻. 186-189 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 都倉 隆: ""回転培養法による後期胚盤胞の全胚培養"" 日本受精着床学会雑誌. 第8巻. 112-114 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 後藤 康夫: ""初期胚発生に及ぼすSODの作用機序"" 日本受精着床学雑誌. 第8巻. 89-91 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 塩谷 雅英: ""ヒト子宮内膜におけるSuperoxide Dismutase(SOD)の発現とその生理的意義"" 日本受精着床学会雑誌. 第8巻. 229-230 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 野々垣 多加史: ""マウス体外受精ー胚培養系に及ぼすSODの効果及びその発現時期"" 日本受精着床学会雑誌. 第8巻. 79-81 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 成本 勝彦: ""ヒト卵管・卵巣におけるSuperoxide Dismutase(SDD)の発現とその生理的意義"" 日本受精着床学会雑誌. 第8巻. 158-160 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 夏山 知: "マウス前核期胚との共培養における琢及び家兎卵管培養細胞の初期胚発生促進効果" 日本受精着床学会雑誌. 第8巻. 98-100 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 岸 淳二: ""各種培養条件下でのラット前核期胚発育の検討"" 哺乳動物卵子学会雑誌. 第8巻. 73-74 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 夏山 知: ""ホロ型及びアポ型トランスフェリンのマウス初期胚発生に及ぼす影響"" 哺乳動物卵子学会雑誌. 第8巻. 103-104 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 塩谷 雅英: ""ヒト卵巣におけるSuperoxide dismutaseの発現ー卵および卵胞成熟との関連の観点からー"" 哺乳動物卵子学会雑誌. 第8巻. 117-118 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 野田 洋一: ""哺乳類初期発生に及ぼす環境因子の解折"" プロシ-ディングス「フリ-ラジカルの臨床」. Vo1.5. (1992)

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      1991 Annual Research Report
  • [Publications] 都倉 隆: ""着床期以降のマウス初期胚に及ぼす酸化的ストレスの影響"" 日本受精着床学会雑誌.

    • Related Report
      1991 Annual Research Report
  • [Publications] Goto,Y.: "Culture of embryos in ductal organs,fallopian tube,vein and ureter" Journal of Clinical Electron Microscopy. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Tokura,T.: "Ultrastructural study of mouse embryos after the implantation stage in vitro:effect of superoxide dismutase" Journal of Clinical Electron Microscopy. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Noda,Y.: "Superoxide dismutase and its role in reproduction." Journal of in Vitro Fertilization and Embryo Transfer. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Noda,Y.: "Improvement of developmental efficiency in rodent embryos in vitro by protecting from oxidative stress." Molecular Reproduction and Development. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Nonogaki T.: "Localization of CuーZuーsuperoxide dismutase in the human male genital organs." Human Reproduction. 7. 81-85 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Goto,Y.: "Pregnacy achieved by transferring blastocysts into endometrial stroma in mice." Human Reproduction. (1992)

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      1991 Annual Research Report
  • [Publications] R.Marino: "Purification and Characterization of A Vitelline Coat Lysin from Ciona intestinalis Spermatozoa" Mol.Reprod.Develop.(1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] H.Kubo: "A Novel Ceramide Trihexoside from the Eggs of the Sea Urchin,Hemicentrotus pulcherrimus" J.Biochem.(1992)

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      1991 Annual Research Report
  • [Publications] H.Kubo: "A Novel Difucosylated Neutral Glycosphingolipid from the Eggs of the Sea Urchin,Hemicentrotus pulcherrmus" J.Biochem.(1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] F.Inagaki: "A Novel Fucosylglycolipid from the Eggs of the sea Urchin,Hemicentrotus pulcherrimus as Determined by Two Dimensional" Nuclear Magnetic Resonance. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 野田 洋一: ""哺乳類初期胚発生に及ぼす環境因子の解折"" 日本産科婦人科学会 熊本地方部会雑誌. 第35巻. 71-76 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 野田 洋一: ""初期胚発生に及ぼす環境因子の解折"" 日本受精着床学会雑誌. 第8巻. 23-26 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Noda,Y.: "Analysis of oviductーderived embryonic growth stimulator activity." International Journal of Fertility. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Noda,Y.: "Involvement of superoxide radicals in mouse 2ーcell block." Molecular Reproduction and Development. 28. 356-360 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Nonogaki T.: "Effects of Superoxide Dismutase on Mouse in vitro fertilization and Embryo Culture System." Journal of in Vitro Fertilization and Embryo Transfer. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Goto,Y.: "Oviductーderived embryo growth promoting activityーa comparison of three ductal organsー." Journal of Clinical Electron Microscopy. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Natsuyama,S.: "Relese of mouse twoーcell block by reduction of protein disulfide with thioredoxin from Escherichia coli." Journal of Reproduction and Fertility. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Shiotani,M.: "Ultrastructural study of uterine epithelia in mice at the implantation stage." Journal of Clinical Electron Microscopy. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 毛利 秀雄: "精子の生物学" 東京大学出版会, 157 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 千葉 和義: "生化学的研究におけるマイクロインジェクション「新生化学実験講座 14巻 発生、分化および老化」" 東京化学同人, (1992)

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      1991 Annual Research Report
  • [Publications] M.Hoshi: "Sperm Activation and Acrosome Reaction In “Biology of the Germ Line ーIn Animals and Manー",eds.H. Mohri,M.Takahashi and T.Tachi" Japan Scientific Societies press,Tokyo, (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] M.Hoshi: "Acrosome ReactionーInducing Glycoconjugates in the Jelly Coat of Starfish Eggs Comparative Spermatology,ed.B.Baccetti" (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 星 元紀 毛利秀雄監修、星 元紀、森沢正昭編: "生体反応と受精 「精子学」" 東京大学出版会, (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 星 元紀 永井克孝編: "受精と糖鎖 「細胞社会のグリオバイオロジ-」" 講談社サイエンチフィク, (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 星 元紀 鈴木康夫、安藤進編: "配偶子のガングリオシド 「ガングリオシド実験法」" 学会出版センタ-, (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Noda,Y.: "Biology of Germ Linesーin Animals and Man" SpringerーVerlag, (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 野田 洋一: "モダンクリニカルポイント・産婦人科" 金原出版(株), (1992)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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