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着床と胎盤形成の分子機構

Research Project

Project/Area Number 03207104
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Research InstitutionKyoto University

Principal Investigator

森 崇英  京都大学, 医学部, 教授 (90026865)

Co-Investigator(Kenkyū-buntansha) 江藤 一洋  東京医科歯科大学, 歯学部, 教授 (30014161)
舘 隣  東京大学, 大学院・農学系, 教授 (30011711)
森 庸厚  東京医科学研究所, 助教授 (40012760)
村松 喬  鹿児島大学, 医学部, 教授 (00030891)
竹市 雅俊  京都大学, 理学部, 教授 (00025454)
Project Period (FY) 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥42,000,000 (Direct Cost: ¥42,000,000)
Fiscal Year 1991: ¥42,000,000 (Direct Cost: ¥42,000,000)
Keywordsテラトカルシノ-マ / 卵管上皮 / カドヘリン / マウス全胚培養 / LGL / 細胞外マトリックス / 組織化学 / プロトオンコジ-ン
Research Abstract

本年度の研究計画に基づき以下の如き成果が得られた。1)テラトカルシノ-マ細胞にセレクチンが存在することを明らかにし、新しいカルシウム結合蛋白が存在することをも明らかにした(村松)。2)ウシ卵管上皮の無血清培養上澄に胚盤胞形成促進物質が存在していることを明かにしその部分精製を行った。その結果2種の生理活性物質の存在する事が明かとなった(及川)。3)マウス胚およびハムスタ-卵管との共培養で観察される胚発生促進効果は培養環境にもたらされる相対的な低酸素分圧、上皮細胞から分泌されるLーCys等が作用している事が考えられた(森崇)。4)Pーカドヘリン遺伝子を欠失しているES細胞クロ-ンを得ることに成功した。これを用いて現在正常胚に注入しキメラマウスの作製を試みている(竹市)。5)精子頭部膜上のMHC class II分子と卵黄膜上のCD4様分子の相互作用により精子のシグナルが卵に伝達される事を明らかにした(森庸)。6)マクロファ-ジ培養上澄にマウス胚盤胞の発生を促進する活性を持つ物質(分子量3万以下)を発見し、これの同定を行っている(舘)。7)早期の卵黄嚢解放をせずに全胚盤胞を行いこれに生理活性物質を注入したところEGFにより顔面形成の阻害が生ずる事が判明した(江藤)。8)妊娠初期の脱落膜に存在するLGLは血液由来LGLとは異なることが明らかとなった(内田)。9)胎盤における細胞外マトリックス成分に対するレセプタ-の分布を調べたところインテグリンを介して細胞が接着していることを示された(伊勢村)。10)胚盤胞が子宮内膜に接着すると子宮内膜細胞に脱落膜化が生じ各種の酵素活性が上昇する事が示された(斉藤)。11)CーKit蛋白が出生後のマウス卵細胞、特に減数分裂前期のものに強く発現している事が示された(藤田)。12)MHC classI抗原の妊孕現象に対する関与をトランスジェニックマウス(A_cーL^d、A_cーK^b)を作製し現在効果を検討中である(宮崎)。

Report

(1 results)
  • 1991 Annual Research Report
  • Research Products

    (58 results)

All Other

All Publications (58 results)

  • [Publications] Takeichi,M.: "Cadherin cell adhesion receptors as a morphogenetic regulator." Science. 251. 1451-1455 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Inuzuka,H.;Miyatani,S.;Takeichi,M.: "R-cadherin:A novel Ca^<2+>-dependent cell-cell adhesion molecule expressed in the retina." Neuron. 7. 69-79 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Inuzuka,H.;Redies,C.;Takeichi,M.: "Differential expression of R-and N-cadherin in the nervous system and connective tissues during early chicken development." Development. 113. 959-968 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Hatta,M.;Takeichi,M. 他: "Genomic organization and chromosomal mapping of the mouse Pーcadherin gene." Nucleic Acids Research. 16. 4437-4441 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Hirano,S.;Takeichi,M. 他: "Drosophila PS integrins recognize vertebrate vitronectin and function as cellーsubstrate adhesion receptors in vitro." Development. 113. 1007-1016 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kishi,J.: "Block to development in cultured rat 1-cell embryos is overcome using medium HECM-1." Human Reproduction. 6. 1445-1448 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Nonogaki,T.: "protection of mouse embryos fertilized in vitro from oxidative stress by thioredoxin and superoxide dismutase." Human Reproduction. 6(9). 1305-1310 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Umaoka,Y.: "Developmental potentiality of embryos cultured under low oxygen tension with superoxide dismutase." J.IVR-ET.

    • Related Report
      1991 Annual Research Report
  • [Publications] Kazama,T.;Nakamura,T.;Isemura,M.;Sato,Y.: "Immunohistochemical localization of heparan sulfate-containing proteoglycan demonstrated with monoclonal antibodies in normal human skin:comparison with that of fibronectin" Archives of Dermatological Research. 281. 440-442 (1989)

    • Related Report
      1991 Annual Research Report
  • [Publications] Isemura,M.;Mita,T.;Satoh,K.;Narumi,K.;Motomiya,M.: "Myosin light chain kinase inhibitors ML-7 and ML-9 inhibit mouse lung carcinoma cell attachment to the fibronectin substratum" Cell Biology International Reports. 15. 965-972 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Oguro,K.;Kazama,T.;Isemura,M.;Nakamura,T.;Akai,S.;Sato,Y.: "Immunohistochemical alterations in basement membrane components of squamous cell carcinoma" Journal of Investigative Dermatology. 96. 250-254 (1991)

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      1991 Annual Research Report
  • [Publications] Momozaki,N.et al.: "Suppression of murine leukemia virus-mediated 3Y1 fusion by expression of mouse MHC class I." Arch.Virol.119. 43-52 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Niwa,H.et al.: "Efficient selection for high-expression transfectants with a novel eukaryotic vector." Gene. 108. 193-200 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Suematsu,S.et al.: "Generation of plasmacytomas with the chromosomal translocation t(12;15) in interleukin 6 transgenic mice." Proc.Natl.Acad.Sci.USA. 89. 232-235 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Watanabe,Y.et al.: "A murine thymic stromal cell line which may support the differentiation of CD4-8-thymocytes into CD4+8-αβ T cell receptor positive T cells." Cell.Immunol.

    • Related Report
      1991 Annual Research Report
  • [Publications] Miyamoto,I.et al.: "Mutational analysis of the structure and function of the XPAC protein:identification of essential domains for nuclear localization and DNA excision repair." J.Biol.Chem.

    • Related Report
      1991 Annual Research Report
  • [Publications] Umaoka,Y.: "Effects of Oxygen Toxicity on Early Development of Mouse Embryos." Molecular Reproduction and Development.31. 28-33 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Noda,Y.: "Involvement of superoxide radicals in mouse 2-cell block." Molecular Reproduction and Development. 28. 356-360 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Mori,E.et.al.: "Neutral oligosaccharide structures linked to asparagines of porcine zona pellucida glycoproteins" Biochemistry. 30. 2078-2087 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Mori,T.et.al.: "Expression of CD4-like structure on murine egg vitelline membrane and its signal transductive roles through p561ck in fertilization" Am.J.Reprod.Immunol.

    • Related Report
      1991 Annual Research Report
  • [Publications] Mori,T.et.al.: "Expression of the signal transducing region of CD4-like and lck genes in murine egg." Biochem.Biophys.Res.Commun.182. 527-533 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Mori,T.: "Cellular immunity" Rinsho Fujinka Sanka. 46. 142-144 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Munakata,H.;Hayashi,N.;Yamaguchi,Y.;Aoyagi,Y.;Ichida,F.;Isemura,M.: "Biochemical evidence for the local synthesis and retention of fibronectin in the tissue of human placenta" International Journal of Biochemistry. 20. 113-116 (1988)

    • Related Report
      1991 Annual Research Report
  • [Publications] Takamoto,M.;Endo,T.;Isemura,M.;Kochibe,N.;Kobata,A.: "Structures of asparagine-linked oligosaccharides of human placental fibronectin" Journal of Biochemistry. 105. 742-750 (1988)

    • Related Report
      1991 Annual Research Report
  • [Publications] Mori,T.,Takakura,K.,Narimoto,K.,Kariya,M.,Imai,K.,Fujiwara,H.,Okamoto,N.,Kariya,Y.,Shiotani,M.,Umaoka,Y.,Noda,Y.,and Uchida,A.: "Endocrine and immune imphcations of human endometrial decidualization in implantaion.In:Frontiers in Human Reproduction." M.Seppala,L.Hamberger,ed.,Ann.New York Acad.Sci.626. 321-330 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Okamoto,N.,Takakura,K.,Kariya,Y.,Imai,K.,Kariya,M.,Emi,N.,Kanzaki,H.,Uchida,A,and Mori,T.: "Effects of placental protein 14(PP14)and insulin-like growth factor-binding protein-1(IGFBP-I)on NK cell activity and T cell proliferative response." Proc.Jap.Soc.Basic and Clin.Reprod.Immunol.(1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 内田 温士: "NK細胞の基礎と臨床." エンドメテリオ-シス研究会誌. 12. 8-12 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] H.Abe and T.Oikawa: "Resional differences in the ultrastructural features of secretory cells in the golden hamster oviductral epithelium." J.Anatomy. 175. 147-158 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] H.Abe,K.Ookata,M.Abe and T.Oikawa: "Immunological characterization of oviductal glycoproteins associated with the zona pellucida of the golden hamster egg." J.Exp.Zool.(1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] H.Abe and T.Oikawa: "Examination by scanning electron microscopy of the oviductal epithelium of prolific Chainese Meishan pig at the follicular and luteal phases." Anatomical Record.(1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] K,Kobayashi,Y.Takagi,T.Satoh,H.Hoshi and T.Oikawa: "Development of early bovine embryos to the blastocyst stage in serumfree conditioned medium from bovine granulosa cells." In Vitro Cell & Dev.Biol.(1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] H.Hoshi,M.Onodera and T.Oikawa: "Isolation,cell characterization,and growth regulation of bovine oviduct epithlial cells in vitro." Tissue Culture Res.Commun.(1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Takizawa,T.& T.Saito: "Freeze substitution fixation for enzyme istochemistry." Acta Histochem.Cytochem.22. 139-151 (1989)

    • Related Report
      1991 Annual Research Report
  • [Publications] Saito,T.& T.Takizawa: "The identification of an active enzyme site by raid free substitution enbzyme histochemistry on rat retina." Acta Histochem.Cytochem.24. 121-132 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Horie,K.et al.: "The expression of c-kit protein during oogenesis and early embryonic development" Biology of Reproduction. 45. 547-552 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kaneko,Y.et al.: "Adhesion of mouse mast cells to fibroblasts:Adverse effects of Steel (Sl) mutation" Journal of Cellular Physiology. 147. 224-230 (1991)

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      1991 Annual Research Report
  • [Publications] Dubreuil,P.et al.: "The c-fms gene complements the mitogenic defect in mast cells derived from mutant W mice but not mi mice" Proceedings of National Academy of Sciences USA. 88. 2341-2345 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Takenawa,J.et al.: "Enhanced expression of interleukin-6 in primary human renal cell carcinoma" Journal of Nationl Cancer Institute. 83. 1668-1672 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kadowaki,S.et al.: "Prevention of genetic resistance to bone marrow allografts by reticuloendothelial system blockade" Biomedicine and Pharmacotherapy. 45. 417-422 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Okamoto,N.,Uchida,A.Takakura,K.,Kariya,Y.,Kanzake,H.,Rutanen,Koistinen,R.,Sapala,M.,and Mori,T.: "Suppression by human placental protein 14 on natural killer cell activity and T cell proliferative response." Am.J.Reprod.Immunol.(1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 朝田 総一郎他: "哺乳類全胚培養法を用いた顔面形態形成の研究" 組織培養. 16. 45-50 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] Takashi Muramatsu: "Subcellular localization of N-acetylglucosaminide β1→4 galactosyltransferase revealed by immunoelectron microscopy" J.Histochem.Cytochem.39. 299-309 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Takashi Muramatsu: "A new family of heparin binding factors:strong conservation of midkine (MK) sequences between the human and the mouse" Biochem.Biophys.Res.Commun.176. 792-797 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Takashi Muramatsu: "Purification of recombinant midkine and examination of its biological activities:functional comparison of new heparin binding factors" Biochem.Biophys.Res.Commun.177. 652-658 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Takashi Muramatsu: "Immunohistochemical localization of the carbohydrate antigen 4C9 in the mouse embryo:a reliable marker of mouse primordial germ cells" Differentiation. 48. 75-82 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Takashi Muramatsu: "Structual studies on a binding site for Dolichos biflorus agglutinin in the small intestine of the mouse" J.Biochem.109. 178-183 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] C.Tachi: "Partial characterization of mecromolecular components in fetal bovine serum reqired for development of mouse blastocysts cultured in vitro." Dev.Growth Deff.34. 66-76 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] R.ーZ.Lu 他: "Histochemical demonstration of activin/inhibin βAー,βBー and αーsubunits in early embryos and oviducts of diferent strains of mice." J.Reprod.Dev.38. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] K.Ymanouchi他: "Spleen macrophages enhance prolactinーinduced progest in secretion from mature rat granulosa cells in vitro." Biol.Reprod.46. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] S.Matsuyama他: "Splenic macrophages enhance prolactin and luteinizing hormone actin in rat luteal cell cultures" Endocrinol.Japon.39. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] S.Adada他: "Differential yolk sac and facial development in cultured C57BL/6 mouse embryos." Development,Growth & Differentiation. 38. 409 (1989)

    • Related Report
      1991 Annual Research Report
  • [Publications] K.Eto他: "The observation of developing epithelium in the mouse nasal placode with SEM." J.Electron Microscopy. 38. 279 (1989)

    • Related Report
      1991 Annual Research Report
  • [Publications] N.OsumiーYamashita他: "The cell surface development and actin distribution of epithelium during facial morphogenesis." Cell Differentiation and development (Supple). 27. S46 (1989)

    • Related Report
      1991 Annual Research Report
  • [Publications] Y.Fukiishi他: "The effect of protamine on rat embryo in culture." Congenital Anomalies. 30. 248 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] 齋藤 多久馬: "初期発生と形態形成 新生理学大系:発生分化の生理学" 医学書院, 17-37 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Shinohara,K.,Yada,K.,Kihara,H.& Saito,T.: "X-ray Microscopy in Biology and Medicine" Japan Scientific Societs Press Springer-Verlag, 336 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] Mori,T.: "Immunobiological studies on the mammalian fertilization and implantation.In "Biology of Germ Lines-in Animals and Man"" Jap.Sci.Soc.Press, (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 齋藤 多久馬,荒木 正介: "バイオサイエンス戦略マニュアル:新しい素材とマ-カ-・ブロ-ブ 個体発生のマ-カ-:脊椎動物" 共立出版, 283-290 (1990)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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