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大腸菌ゲノムの一次構造の解析

Research Project

Project/Area Number 03221105
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Research InstitutionKobe University

Principal Investigator

磯野 克己  神戸大学, 理学部, 教授 (70011640)

Co-Investigator(Kenkyū-buntansha) 加納 康正  京都薬科大学, 生命薬学研究所, 助教授 (50093399)
和田 明  京都大学, 理学部, 助手 (80025387)
藤田 信之  国立遺伝学研究所, 分子遺伝, 助手 (90173434)
椙崎 弘幸  京都大学, 化学研究所, 助手 (60135598)
水野 猛  名古屋大学, 農学部, 教授 (10174038)
Project Period (FY) 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥18,700,000 (Direct Cost: ¥18,700,000)
Fiscal Year 1991: ¥18,700,000 (Direct Cost: ¥18,700,000)
Keywords大腸菌デ-タベ-ス / 塩基配列決定の高速化 / 湾曲DNA / 転写関始シグナル / 塩基性タンパク質
Research Abstract

本重点領域研究の計画研究をより円滑に進めるために、一昨年度作製した「大腸菌デ-タベ-ス」の利用プログラムをより一層改良し、Version3.0として班員に配付した。この改良により、各班員がデ-タの訂正を行なうことができるようになり、「大腸菌デ-タベ-ス」がより完全なものになることが期待できる。さらに、新しい塩基配列デ-タ(GenBank70.0)を取り入れ、同時に、各種デ-タの誤りを訂正した。また、塩基配列決定法の改良については、一昨年度来進めてきたトランスポゾンを利用する塩基配列決定法を一段と改良し、PCRを利用しDNAを増幅すること、その際、プライマ-の一端に常磁性のビ-ズを結合させることにより、一本鎖DNAな回収を容易にすること、などの改良を図り、この方法を用いて約80kbの塩基配列決定を行なった。一方、大腸菌ゲノムのもつ各種の構造的ならびに機能的特色についても昨年度にひき続き解析を進めた。その結果、大腸菌のヒストン様タンパク質の遺伝子が自己制御を行なっていること、湾曲DNAに特異的に結合するタンパク質がhns遺伝子の産物であることがわかったこと、定常期のRNAポリメラ-ゼが対数増殖期のものと異なっていること、塩基性タンパク質についてのN末端のアミノ酸配列決定を進め、数種の新しいタンパク質を見出したことなどの成果を挙げている。

Report

(1 results)
  • 1991 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Uemura,Y.: "Cloning,characterization and physical location of the rplY gene which encodes ribosomal protein L25 in Escherichia coli K-12." Mol.Gen.Genet.226. 341-344 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kitakawa,M.: "The mitochondrial ribosome." Biochimie. 73. 813-825 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Yura,T.: "Systematic sequencing of the Escherichia coli genome:Analysis of the 0-2.4 min region." Science. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kasai,H.: "Use of a mini-transposon and magnetic beads in PCR-based systematic DNA sequencing of Escherichia coli ordered clones." (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Jeong,Z.: "Nucleotide sequence and analysis of an Escherichia coli chromosomal region containing the genes for ribosomal proteins L21 and L27." (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Isono,K.: "Escherichia coli database and its retrieval system:Quick access to genetic and physical maps,ordered clones,nucleotide sequences and related literature." (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Hiroyuki Sugisaki;Katsuhiko Yamamoto;Mituru Takanami: "The HgaI Restriction-Modification System Contains Two Cytosine Methylase Genes Responsible for Modification of Different DNA Strands" The Journal of Biological Chemistry. 266. 13952-13957 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Yamada,H.,et al: "Molecular analysis of the Escherichia coli hns gene encoding a DNA-binding protein,which preferentially recognizus curved DNA sequences." Mol.Gen.Genet.230. 332-337 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Tanaka,K.,et al: "Systematic characterization of curved DNA segments randomly cloned from Escherichia coli and their functional significance" Mol.Gen.Genet.226. 367-376 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Tanaka,K.et al.: "Overproduction and rapid purification of the Escherichia coli histone-like protein,H-NS." Agric.Biol.Chem.55. 3139-3141 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Igarashi,K.,Fujita,N.,& Ishihama,A.: "Identification of a subunit assembly domain in the alpha subunit of Escherichia coli RNA polymerase" Journal of Molecular Biology. 218. 1-8 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Nakayama,M.,Fujita,N.,Osawa,S.& Ishihama,A.: "Identification and characterization of RNA polymerase sigma factor from Micrococcus luteus" Journal of Biological Chemistry. 266. 2911-2916 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Ozaki,M.,Wada,A.,Fujita,N.,& Ishihama,A.: "Growth phase-dependent modification of RNA polymerase in Escherichia coli" Molecular and General Genetics. 230. 17-23 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Ozaki,M.,Fujita,N.,Wada,A.,& Ishihama,A.: "Promoter selectivity of the stationary-phase forms of Escherichia coli RNA polymerase and conversion in vitro of the Sl form enzyme into a log-phase enzyme-like form" Nucleic Acids Research. 20. 257-261 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Miyazawa,S.,Makivic,M.,Horiki,T.and Homma,S.: "Numerical Expreiments on Frustrated Quantum Spin Systems" in"Computational Approach in Condensed Matter Phenomena",Tokyo:Springer Verlag. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Miwako Ozaki;Akira Wada;Nobuyuki Fujita & Akira Ishihama: "Growth phase-dependent modification of RNA polymerase in Escherichia coli" Mol Gen Gent. 230. 24-27 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Miwako Ozaki,Nobuyuki Fujita,Akira Wada & Akira Ishihama: "Promoter selectivity of the stationary-phase forms of Escherichia coli RNA polymerase and conversion in vitro of the Sl form enzyme into a log-phase enzyme-like form" Nucleic Acids Research. 20. 257-261 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kano,Y.: "Participation of the histone-like protein HU and of IHF in minichromosomal maintenance in Escherichia coli." Gene. 103. 25-30 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kenri,T.: "Construction and characterization of an Escherichia coli mutant with a deletion of the metZ gene encoding tRNA_<f1>^<met>." Gene. 103. 31-36 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kenri,T.: "Construction and characterization of an Escherichia coli mutant deficient in the metY gene encoding tRNA^<Met>_<f2>:either tRNA^<Met>_<f1> or ^<Met>_<f2> is required for cell growth" Gene.

    • Related Report
      1991 Annual Research Report
  • [Publications] Kohno,K.: "Autogenous regulation of transcription of the hupA gene in Escherichia coli" Proc.Natl.Acad.Sci.USA.

    • Related Report
      1991 Annual Research Report
  • [Publications] Goshima,N.: "HU-2 Mutants of Escherichia coli deficient in DNA binding"

    • Related Report
      1991 Annual Research Report
  • [Publications] Goshima,N.: "Increased ability of DNA-binding of HU-2 by the amino acid substitution in the carboxy-terminal arm domain."

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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