| Project/Area Number |
03303014
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| Research Category |
Grant-in-Aid for Co-operative Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
結晶学
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| Research Institution | Nagoya University |
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Principal Investigator |
YAMANE Takashi Nagoya University,School of Engineering, Associate Professor, 工学部, 助教授 (80030055)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUMOTO Osamu Tokyo Institute of Technology, Faculty of Bioscience and Biotechnology, Res. Ass, 生命理工学部, 助手 (10231599)
HARADA Shigeharu Osaka University, Faculty of Engineering, Assistant Professor, 工学部, 講師 (80156504)
FUKUYAMA Keiichi Osaka University, Faculty of Science, Assoc. Prof., 理学部, 助教授 (80032283)
MATSUURA Yoshiki Osaka University, Institute for Protein Research, Assoc. Prof., 蛋白質研究所, 助教授 (90029968)
IIJIMA Shinji Nagoya University, School of Engineering, Professor, 工学部, 教授 (00168056)
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| Project Period (FY) |
1991 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥9,500,000 (Direct Cost: ¥9,500,000)
Fiscal Year 1992: ¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 1991: ¥5,600,000 (Direct Cost: ¥5,600,000)
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| Keywords | alpha-amylase / lysozyme / malto-tetraose(G4) forming amylase / malto-pentaose(G5) forming amylase / beta-galactosidase / cellobiohydrolase / crystal structure / molecular replacement / アミラーゼインヒビター / セルラーゼ / アミラ-ゼ / アミラ-ゼインヒビタ- / キシラナ-ゼ / リゾチ-ム / マルトペンタノ-ス生成酵素 / リポアミドデヒドロゲナ-ゼ / ガラクトシダ-ゼ |
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| Research Abstract |
The crystal structure analyses of the enzymes concerned with glycolysis have been carried out during 1991-1992 with the financial support of this Grant-in-Aid. The molecular structures of xylanase and cellulase by Dr. Hata, malto-teraose(G4) forming exo-amylase by Dr. Matsuura, bacterial lysozyme by Dr. Harada, were determined. The functions of objective enzymes were made clear on the basis of their structures. The advanced crystallographic studies were promoted on bacterial alpha-amylases by Dr. Yamane, alpha-amylase inhibitor by Dr. Fukuyama, and malto-pentaose (G5) forming amylase by Dr. Hamada, etc. The crystallization techniques of proteins were also improved, for example, by Drs. Harada and Highchi. For the structure analysis, the methodology on the molecular replacement method was made a great advance by Drs. Yamane, Tanaka, etc. and the know-how was published in the collective research reports. The improvement of the hardwares and softwares to collect and process the X-ray data more rapidly was carried out by Prof. Satow and Drs. Tanaka and Morimoto. The cloning of beta-galactosidase and cellobiohydrolase in prokaryotic cells could be executed by Prof. Iijima, in order to use biomass energy resouces effectively.
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