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BASIC STUDIES FOR TECHNICAL DEVELOPMENTS ON SEED PRODUCTION IN AQUACULTURE

Research Project

Project/Area Number 03304020
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field General fisheries
Research InstitutionTOKYO UNIVERSITY OF FISHERIES

Principal Investigator

TAKASHIMA Fumio  TOKYO UNIV.OF FISHERIES, FAC. OF FISHERIES, PROFESSOR, 水産学部, 教授 (60041703)

Co-Investigator(Kenkyū-buntansha) KANAZAWA Akio  KAGOSHIMA UNIV., FAC. FISHEIRES, PROF.,, 水産学部, 教授 (70041697)
AOKI Takashi  MIYAZAKI UNIV., FAC. AGRICUL., PROF., 農学部, 教授 (00051805)
WAKABAYASHI Hisatsugu  UNIV. TOKYO, FAC. OF AGRICUL., PROF., 農学部, 教授 (00011932)
MUROGA Kiyokuni  HIROSHIMA UNIV., FAC. APPL. BLOL. SCI., PROF., 生物生産学部, 教授 (30011993)
SUZUKI Yuzuru  UNIV. OF TOKYO, FAC, OF AGRICUL., ASSOC. PROF.,, 農学部, 助教授 (40107412)
出口 吉昭  日本大学, 農獣医学部, 教授 (60058906)
小川 和夫  東京大学, 農学部, 助教授 (20092174)
Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥16,800,000 (Direct Cost: ¥16,800,000)
Fiscal Year 1992: ¥6,800,000 (Direct Cost: ¥6,800,000)
Fiscal Year 1991: ¥10,000,000 (Direct Cost: ¥10,000,000)
KeywordsSEEDS FOR AQUACULTURE / DEVELOPMENT / PATHOGENS / STARVATION / VIRUS / IMMUNE RESPONSE / OZONE / GENE MANIPULATION / キャッチ-アップ現象 / リンパ球 / 胸腺 / IgM / 色素異常 / オキシダント / シマアジ / キジハタ / マダイ / ガザミ / 仔稚 / 免疫グロブリン / 母子免疫
Research Abstract

The main purposes of this study are to obtain basic informations about the causes and mechanism of early death during larval and young fish used aquaculture as the seed for aquaculture. We have investigated from pathogenic, diagnostic,immunological, physiological, nutritional and environmental points of view and obtained the following results.
1). There are mass mortalities during seed production due to infectious diseases. Among these problems, the viral diseases such as SJNNV in the striped jack and YAV in the yellowtail are serious problem at present. It has become clear that possible courses of the sJnnv( viral nervous necrosis in larval striped jack) are their parental spawners.
2). fish larvae encountering food particles some time after or during the period of yolk absorption grow normally. If suitable food is not available for longer periods, the larvae may attained state of irreversible inanition, at the end of which mortality occurs. Larvae entering this terminal phase are said … More to have reached the PNR( point-of-no-return). For practical purposes, PNR may be defined as the maximum delay in first feeding that still enables 50 % of the larvae to survive. Some examples of starvation-induced mortality and PNR in various fish species are presented.
3). Successful use of ozone as a disinfectant for aquaculture system was developed. Bacterial counts of all fish pathogens decreased at more than 0.04-0.06 mg of total residual oxidans(TRO) per liter. This result indicates that ozone treatment at more than 1.0 mg of TRO/1 for several minutes is able to disinfect seawater for mariculture efficiently.
4).In order to augment the genetic properties of the seed, transgenenic method has been tried on trout eggs and confirmed that foreign genes introduced during egg stage become active in the offsprings. At the same time,ploidy manipulations have been done and got the following results; the variation in the maximum number of nucleoli per cell is related to their age. Diploid and triploid hybrids with 12 combinations have been produced by the crosses between female rainbow trout and another salmonids fishes. In addition, hybrids females among 3 species of medaka have been produced( trigenomic allotriploids). Less

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (60 results)

All Other

All Publications (60 results)

  • [Publications] G.Yoshizaki: "Induction of carp α-globin gene into rainbow trout." Nippon Suisan Gakkaishi. 57. 819-824 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 邱 加二: "オオクチバスの成熟と稚熟" 水産増殖. 39. 343-351 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 村井 衛: "シマアジ仔稚魚の鰭と鯖の発育" 水産増殖. 39. 201-210 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 川辺 勝俊: "シマアジ卵発生に及ぼす水温の影響" 水産増殖. 39. 211-216 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] C.Odan: "Estimation of effective condition for induction of triploidy in goldfish,Carassius auratus." J.Tokyo Univ.Fish..78. 127-135 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] とう 亜光: "ニジマス同質および異質倍数体の養殖特性について" 水産増殖. 40. 121-129 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 川辺 勝俊: "シマアジ卵の最適ふ化塩分および水温" 水産増殖. 40. 261-268 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 村井 衛: "人工採苗シマアジ仔稚魚の相対成長" 水産増殖. 40. 253-259 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] G.Yoshizaki: "Introductoin and expression of CAT gene in rainbow trout." Nippon Suisan Gakkaishi. 58. 1659-1665 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] J.Kurita: "メダカ完全異質3倍体の作成" 日本水産学会誌. 58. 2311-2314 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] C.Odan: "Variation in the maximum number of nucleoli in diploid and triploid common carp" Nippon Suisan Gakkaishi. 58. 2307-2309 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] C.A.Strussmann: "Larval growth and point-of-no-return in fish." Oceanis. 18. 27-39 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Feng Zhang: "Fertility of triploid backcross progeny" Japan.J.Ichthyol..39. 229-233 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Feng Zhang: "Chromosome synapsis and recombination during meiotic division in gynogenetic triploid ginbuna." Japan.J.Ichthyol..39. 151-155 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] K.Mori: "Properties of a new virus belonging to nodaviridae found in larval striped jack with nervous necrosis." Virology. 187. 368-371 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] K.Mushiake: "Detection of striped jack nervous necrosis virus by enzyme-linked immunosorbent assay." Fish Pathol..27(4). 191-195 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] M.Arimoto: "Detection of antibodies against striped jack nervous necrosis virusfrom brood stocks of striped jack." Nippon Suisan Gakkaishi. 58. 2351-2356 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] K.Muroga: "Bacterial and viral diseases of marine fish during seed-production" NOAA techn.Rep..111. 57-61 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] K.Muroga: "Hatchery disiases of marine fish in Japan." Disease in Asian Aquacul.I. 215-222 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] H.Sugita: "Applicatin of ozone disinfection to remove Enterococcus seeriolicida.Pasteurell apsicicida.and Vibrio anguillarum from sea water." Appl.Environ.Microbiol..58. 4072-4075 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 杉田 治男: "海水中の残留オキシダントの簡易測定法" 水産増殖. 40. 45-49 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] G. Yoshizaki: "Induction of carp alpha-globin gene into rainbow trout." Nippon Suisan Gakkaishi. 57. 819-824 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] C. Odan: "Estimation of effective condition for induction of triploidy in goldfish.Carassius auratus." J.Tokyo Univ.Fish.78. 127-135 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] G. Yoshizaki: "Introduction and expression of CAT gene in rainbow trout" Nippon Suisan Gakkaishi. 58. 1659-1665 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] C. Odan: "Variation in the maximum number of nucleoli in diploid and triploid common carp" Nippon Suisan Gakkaishi. 58. 2307-2309 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] C.A.Strussmann: "Larval growth and point-of-no-return in fish." Oceanis. 18.1. 27-39 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Feng Zhang: "Fertility of triploid backcross progeny" Japan. J. Ichthyol.39. 229-233 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Feng Zhang: "Chromosome synapsis and recombination during meiotic division in gynogenetic triploid ginbuna." Japan. J. Ichthyol.39. 151-155 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] K. Mori: "Properties of a new virus belonging to nodaviridae found in larval striped jack with nervous neerosis." Virology. 187. 368-371 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] K. Mushiake: "Detection of striped jack nervous necrosis virus by enzyme-linked immunosorbent assay." Fish Pathol.27(4). 191-195 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] M. Arimoto: "Detection of antibodies against striped jack nervous necrosis virusfrom brood stocks of striped jack." Nippon Suisan Gakkaishi. 58. 2351-2356 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] K. Muroga: "Bacterial and viral diseases of marine fish During seed-production" NOAA techn.Rcp.111. 57-61 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] K. Muroga: "Hatchery diseases of marine fish in Japan." Disease in Asian Aquacul.I. 215-222 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] H. Sugita: "Application of ozone disinfection to remove Enterococeus seerioliceda. Pasteurell apsicicida, and Vibrio anguillarum from sea water." Appl.Environ.Microbiol.58. 4072-4075 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] G.Yoshizaki: "Induction of carp a-globin gene into rainbow trout." Nippon suisan Gakkaishi. 57. 819-824 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] 邱 嘉仁: "オオクチバスの成熟と催熟" 水産増殖. 39. 343-351 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] 村井 衛: "シマアジ仔稚魚の鰭と鱗の発育" 水産増殖. 39. 201-210 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] 川辺 勝俊: "シマアジ卵発生に及ぼす水温の影響" 水産増殖. 39. 211-216 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] C.Odan: "Estimation of effective condition for induction of triploidy in goldfish,Carassius auratus." J.Tokyo Univ.Fish.,. 78. 127-135 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] 亜光: "ニジマス同質および異質倍数体の養殖特性について" 水産増殖. 40. 121-129 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 川辺 勝俊: "シマアジ卵の最適ふ化塩分および水温" 水産増殖. 40. 261-268 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 村井 衛: "人工採苗シマアジ仔稚魚の相対成長" 水産増殖. 40. 253-259 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] G.Yoshizaki: "Introductoin and expression of CAT gene in rainbow trout" Nippon Suisan Gakkaishi. 58. 1659-1665 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] J.Kurita: "メダカ完全異質3倍体の作成" 日本水産学会誌. 58. 2311-2314 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] C.Odan: "Variation in the maximum number of nucleoli in diploid and triploid common carp" Nippon Suisan Gakkaishi. 58. 2307-2309 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] C.A.Strussmann: "Larval growth and point-of-no-return in fish." Oceanis. 18. 1,27-39 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] Feng Zhang: "Fertility of triploid backcross progeny" Japan.J.Ichthyol.,. 39. 229-233 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] Feng Zhang: "Chromosome synapsis and recombination during meiotic division in gynogenetic triploid ginbuna." Japan.J.Ichthyol.,. 39. 151-155 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] K.Mori: "Properties of a new virus belonging to nodaviridae found in larval striped jack with nervous necrosis." Virology. 187. 368-371 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] K.Mushiake: "Detection of striped jack nervous necrosis virus by enzyme-linked immunosorbent assay." Fish Pathol.,. 27(4). 191-195 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] M.Arimoto: "Detection of antibodies against Striped jack nervous necrosis virusfrom brood stocks of striped jack." Nippon Suisan Gakkaishi. 58. 2351-2356 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] K.Muroga: "Bacterial and viral diseases of marine fish during seed-production" NOAA techn.Rep.,. 111. 57-61 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] K.Muroga: "Hatchery desiases of marine fish in Japan." Disease in Asian Aquacul.I. 215-222 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] H.Sugita: "Application of ozone disinfection to remove Enterococcus seeriolicida.Pasteurell apsicicida,and Vibrio anguillarum from sea water." Appl.Environ.Microbiol.,. 58. 4072-4075 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 杉田 治男: "海水中の残留オキシダントの簡易測定法" 水産増殖. 40. 45-49 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 青木 宙: "海洋微生物の遺伝子工学" Cell Science. 8. 1-12 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Mori.K,T.Nakai,K.Muroga,M.Arimoto: "Proberties of a new virus belonging to Noda viridae found in lorval striped jack with nervous necrosis." Virology. 187.

    • Related Report
      1991 Annual Research Report
  • [Publications] Mori.K,T.Nakai,M.Matsunaga,K.Muroga: "A viral disease in hatcheryーreared larvae and juvenile of red spotled grouper." Fish Pathology. 26. 209-210 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] G.Yoshizaki,T.Oshiro,F.Takashima: "Introduction of carp α‐globrin into rainbow trout" 日本水産学会誌. 57. 819-824 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 隆島 史夫: "水産生物化学(分担)" 東京大学出版会, 236 (1991)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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