| Project/Area Number |
03304024
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| Research Category |
Grant-in-Aid for Co-operative Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
基礎獣医学
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| Research Institution | THE UNIVERSITY OF TOKYO |
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Principal Investigator |
TAKAHASHI Michio THE UNIVERSITY OF TOKYO FACULTY OF AGRICULTURE. PROFESSOR, 農学部, 教授 (30011943)
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| Co-Investigator(Kenkyū-buntansha) |
TATSUYAMA Shigemi THE UNIVERSITY OF TOKYO FACULTY OF AGRICULTURE, ASSISTANT PROFESSOR, 農学部, 助手 (80219526)
MORI Yuji THE UNIVERSITY OF TOKYO FACULTY OF AGRICULTURE, ASSOCIATE PROFESSOR, 農学部, 助教授 (40157871)
NISHIHARA Masugi THE UNIVERSITY OF TOKYO FACULTY OF AGRICULTURE, ASSOCIATE PROFESSOR, 農学部, 助教授 (90145673)
KURAKAMI Noboru MIYAZAKI UNIVERSITY FACULTY OF AGRICULTURE, ASSISTANT PROFESSOR, 農学部, 助手 (80150192)
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| Project Period (FY) |
1991 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥17,800,000 (Direct Cost: ¥17,800,000)
Fiscal Year 1992: ¥5,400,000 (Direct Cost: ¥5,400,000)
Fiscal Year 1991: ¥12,400,000 (Direct Cost: ¥12,400,000)
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| Keywords | HYPOTHALAMUS / VENTROMEDIAL NUCLEUS / FEEDING BEHAVIOR / SEX BEHAVIOR / GONADOTROPIN / EXCITATORY AMINO ACIDS / SYMPATHETIC NERVOUS SYSTEM / TUMOR NECROSIS FACTOR / 多ニュ-ロン発射活動 / 脳室液 / アミノ酸 / 腫瘍壊死因子‐α |
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| Research Abstract |
In this study, we examined neuronal machanisms in the hypothalamus controlling feeding behavior and reproductive function by means of multiunit activity recording technique, water-absorbent polymer injection into the brain, and neuronal cell culture. We found that endogenous excitatory amino acids acting on the Kainate-type receptor in the ventromedial nucleus of the hypothalamus (VMH) facilitated both running activity and the sympathetic nervous system, and that the release of such amino acids from the nerve terminal was presynaptically inhibited by GRBA. The VMH may issue commands to supply energy substrates during the hyper-running and thus integrate both exercise and energy metabolism at the same time. We also found that the electrical activity of the hypothalamic gonadotropin releasing hormone (GnRH) Pulse generator was facilitated by norepinephrine and inhibited by opioid peptides. Further, GnRH Pulse generator activity was found to be suppressed by tumor necrosis factor, a cytokine released from macrophages, via a prostaglandin mediated mechanism. It was suggested that preovulatory gonadotropin surge was induced by a neuronal mechanism that is intrinsically different from the GnRH pulse generator. We established a dissociated rat suprachiasmatic nucleus cell culture which provides a useful model for the analysis of a mammalian circadian system at the cellular level. We identified the 64-KDa protein which may be a part of the circadian clock mechanism. Through these studies, we will be able to resolve problems concerning metabolism and reproduction in the field of veterinary and medical sciences.
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