| Project/Area Number |
03304025
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| Research Category |
Grant-in-Aid for Co-operative Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied veterinary science
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| Research Institution | Obihiro University of Agriculture and Veterinary Medicine |
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Principal Investigator |
SHINAGAWA Morikazu Obihiro Univ., Vet Med., Prof., 畜産学部, 教授 (00001537)
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| Co-Investigator(Kenkyū-buntansha) |
HIRAI Katsuya Gifu Univ.Agricult., Prof., 農学部, 教授 (30021702)
HONDA Eiichi Tokyo Univ.of Agricult. and Tech., Agricult., Ass.Prof., 農学部, 助教授 (20109507)
MIKAMI Takeshi Tokyo Univ.Agricult., Prof., 農学部, 教授 (20091506)
ONUMA Misao Hokkaido Univ.Vet.Med., Prof., 獣医学部, 教授 (70109510)
HORIUCHI Motohiro Obihiro Univ., Vet Med., Instructor., 畜産学部, 助手 (30219216)
石黒 直隆 帯広畜産大学, 畜産学部, 助教授 (00109521)
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| Project Period (FY) |
1991 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥13,600,000 (Direct Cost: ¥13,600,000)
Fiscal Year 1993: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1992: ¥4,700,000 (Direct Cost: ¥4,700,000)
Fiscal Year 1991: ¥6,900,000 (Direct Cost: ¥6,900,000)
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| Keywords | SCRAPIE / SURVEY / PrP^<Sc> / RFLP TYPES / スクレイピー / 海綿状脳症 / PrP遺伝子型 / 伝達性海綿状脳症 / サ-ベイランス |
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| Research Abstract |
1. Restriction fragment length polymorphism (RFLP) on the sheep-PrP gene : To investigate the RFLP types on the PrP-gene, chromosomal DNAs from sheep tissues collected from various districts in Japan were analyzed using restriction endonucleases EcoRI and HindIII and the coding region of sheep PrP as a probe. Sheep were classified into 6 types. Types I to III corresponded to the types reported in England. Nearly half of the scrapie sheep belonged to type I and the frequency of type II in scrapie sheep was low. Distribution of the types in different parts of Japan showed some variations.
2. Survey of scrapie infected sheep in Japan : To survey the incidence of scrapie infection in Japan, detection of PrP^<Sc> in a total of 197 sheep brains, spleens and lymph nodes obtained from various districts in Japan was carried out. PrP^<Sc> was detected in 16 sheep collected in Hokkaido and in 2 obtained from other districts. However, these two sheep had also been produced in Hokkaido. These findings suggest that contamination with scrapie in Japan is restricted to sheep produced in Hokkaido.
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