| Project/Area Number |
03304051
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| Research Category |
Grant-in-Aid for Co-operative Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
物質生物化学
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| Research Institution | The Tokyo Metropolitan Institute of Medical Science |
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Principal Investigator |
YAHARA Ichiro Tokyo Met.Inst.Med.Sci., Vice-Dir., 副所長 (60109957)
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| Co-Investigator(Kenkyū-buntansha) |
FUKASAWA Toshio Keio Univ., Fac.Med., Prof., 医学部, 教授 (90029934)
KAGAWA Yasuo Jichi Med.Sch., Fac.Med., Prof., 医学部, 教授 (30048962)
NAGATA Kazuhiro Kyoto Univ., Inst.Chest Dis., Prof., 胸部疾患研究所, 教授 (50127114)
AIBA Hiroji Nagoya Univ., Fac.Sci., Prof., 理学部, 教授 (20025662)
ISHIHAMA Akira Nat.Inst.Genet., Mol.Genet., Prof., 分子遺伝, 教授 (80019869)
大塚 健三 愛知県がんセンター研究所, 放射線部, 研究員 (40150213)
田島 陽太郎 秋田大学, 医学部, 教授 (30049796)
伊藤 維昭 京都大学, ウィルス研究所, 教授 (90027334)
中村 義一 東京大学, 医科学研究所, 助教授 (40114590)
由良 隆 京都大学, ウィルス研究所, 教授 (20027311)
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| Project Period (FY) |
1991 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥22,300,000 (Direct Cost: ¥22,300,000)
Fiscal Year 1993: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1992: ¥9,000,000 (Direct Cost: ¥9,000,000)
Fiscal Year 1991: ¥10,300,000 (Direct Cost: ¥10,300,000)
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| Keywords | stress response / stress proteins / transcriptional control / molecular chaperone / domain structure / temperature-sensitive mutants / environmental adaptation / cell defense mechanism / ストレス蛋白質 / 翻訳調節 / 熱ショック応答 / 熱ショック蛋白質 / 蛋白質のフォールディング / 蛋白質の分泌 / 転写制御 / 突然変異 / タンパク質間相互作用 / タンパク質キナ-ゼ / 分泌 |
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| Research Abstract |
The synthesis of heat shock proteins in Escherichia coli was shown to be regulated by increasing amounts of the transcription factor sigma^<32>. Functions of E.coli stress proteins involved in DNA replication and secretion have been analyzed using temperature-sensitive mutants. Evidence has been presented that E.coli adapts environmental atresses by modifying both transcriptional and translational machinaries. We also found that heat shock induces relaxation of super-coiled plasmid DNA in E.coli. Stress proteins of thermophilic bacterium were investigated. Stress response of the budding yeast, Saccharomyces cereviseae, was analyzed using various mutants. Domain atructure, stress-induced alteration in biophysical properties, and tissuespecific expression of HSP90 were investigated. Human HSP40 was identified by cDNA cloning to be a homologue of E.coli DnaJ.Function and expression of the collagen-specific chaperone, HSP47, were extensively analyzed.
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