| Project/Area Number |
03304054
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| Research Category |
Grant-in-Aid for Co-operative Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
分子遺伝学・分子生理学
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| Research Institution | Gunma University School of Medicine |
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Principal Investigator |
KOHAMA Kazuhiro Gunma University School of Medicine, Department of Pharmacology, Professor, 医学部, 教授 (30101116)
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| Co-Investigator(Kenkyū-buntansha) |
TAKANO-OHMURO Hiromi Univ.of Tokyo Research associate, 医学部, 助手 (00124470)
ISHIKAWA Ryoki Gunma University Research associate, 医学部, 助手 (20212863)
KURODA Hideyo Toyama Univ.Professor, 理学部, 教授 (50064845)
TANAKA Toshio Mie Univ.Professor, 医学部, 教授 (00135443)
NAGAI Reiko Osaka Univ.Professor, 教養部, 教授 (90028192)
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| Project Period (FY) |
1991 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥25,300,000 (Direct Cost: ¥25,300,000)
Fiscal Year 1993: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1992: ¥10,400,000 (Direct Cost: ¥10,400,000)
Fiscal Year 1991: ¥11,900,000 (Direct Cost: ¥11,900,000)
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| Keywords | Motor protein / Actin / Myosin / Calcium / Regulation / Motility Assay / NMR / Centrifuge Microscope / モーティリティー・アッセイ / アイチン / アクトミオシン / ミオシン軽鎖キナーゼ / カルポニン / カルデスモン / 平滑筋 / シャジク藻 / 原形質流動 / チュブリン / ダイニン / ATP |
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| Research Abstract |
Cell Motility is an activity that is obserbed in the living cells including lower eukayotes, plant cells and animal cells, and is supported by motor proteins. Actomyosin system is a typical motor protein. However, the mode of the interaction of myosin with actin in vitro is so simple that the mode can not explain various phenotypes of cell motility such as cell division, cytoplasmic streaming and contraction.
This co-operative research intended (1) to obtain new motor protein, (2) to find a new mechanism which regulates the interaction of myosin with actin, and (3) to adopt recent techniques for the analysis of cell motility. After the 3 years co-operation among the investigators listed above, following results were obtained. (1) myosin-like proteins were obtained from a few species of plant cells. (2) Novel regulatory role of myosin light chain kinase was elucidated. (3) We applied i)in vitro motility assay to the analysis of the regulation of actomyosin system, ii)protein requence technique to the analysis of acrosomal reaction, iii)NMR technique to motor function of myosin, and iv)centrifuge microscope to motor function of actin.
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