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Role of motor proteins in cell motility

Research Project

Project/Area Number 03304054
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field 分子遺伝学・分子生理学
Research InstitutionGunma University School of Medicine

Principal Investigator

KOHAMA Kazuhiro  Gunma University School of Medicine, Department of Pharmacology, Professor, 医学部, 教授 (30101116)

Co-Investigator(Kenkyū-buntansha) TAKANO-OHMURO Hiromi  Univ.of Tokyo Research associate, 医学部, 助手 (00124470)
ISHIKAWA Ryoki  Gunma University Research associate, 医学部, 助手 (20212863)
KURODA Hideyo  Toyama Univ.Professor, 理学部, 教授 (50064845)
TANAKA Toshio  Mie Univ.Professor, 医学部, 教授 (00135443)
NAGAI Reiko  Osaka Univ.Professor, 教養部, 教授 (90028192)
Project Period (FY) 1991 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥25,300,000 (Direct Cost: ¥25,300,000)
Fiscal Year 1993: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1992: ¥10,400,000 (Direct Cost: ¥10,400,000)
Fiscal Year 1991: ¥11,900,000 (Direct Cost: ¥11,900,000)
KeywordsMotor protein / Actin / Myosin / Calcium / Regulation / Motility Assay / NMR / Centrifuge Microscope / モーティリティー・アッセイ / アイチン / アクトミオシン / ミオシン軽鎖キナーゼ / カルポニン / カルデスモン / 平滑筋 / シャジク藻 / 原形質流動 / チュブリン / ダイニン / ATP
Research Abstract

Cell Motility is an activity that is obserbed in the living cells including lower eukayotes, plant cells and animal cells, and is supported by motor proteins. Actomyosin system is a typical motor protein. However, the mode of the interaction of myosin with actin in vitro is so simple that the mode can not explain various phenotypes of cell motility such as cell division, cytoplasmic streaming and contraction.
This co-operative research intended (1) to obtain new motor protein, (2) to find a new mechanism which regulates the interaction of myosin with actin, and (3) to adopt recent techniques for the analysis of cell motility. After the 3 years co-operation among the investigators listed above, following results were obtained. (1) myosin-like proteins were obtained from a few species of plant cells. (2) Novel regulatory role of myosin light chain kinase was elucidated. (3) We applied i)in vitro motility assay to the analysis of the regulation of actomyosin system, ii)protein requence technique to the analysis of acrosomal reaction, iii)NMR technique to motor function of myosin, and iv)centrifuge microscope to motor function of actin.

Report

(4 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • 1991 Annual Research Report
  • Research Products

    (58 results)

All Other

All Publications (58 results)

  • [Publications] Masuda,T.,et al.: "Confocal laser microscopy of dystrophin localization in guinea pig skeletal muscle fibers." J.Cell Biol.119. 543-548 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Ishikawa,R.,et al.: "Stimulation of the interaction between actin and myosin by Physarum caldesmon-like protein and smooth muscle caldesmon." J.Biol.Chem.266. 21784-21790 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kamitsubo,E.,et al.: "Immobilization of endoplasm flowing contiguous to the actin cables upon electrical stimulus in Nitella internodes." Protoplasma. 168. 82-86 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kobayashi,H.,et al.: "Isolation of cDNA for 155kDa protein exhibiting myosin light chain kinase activity." J.Biochem.112. 786-791 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kohno,T.,et al.: "Partial purfication of myosin from lily pollen tubes by monitoring with in vitro motility assay." Protplasma. 170. 77-85 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Takagi,T.,et al.: "Structure of 29 kDa protein from ascidian (Halocynthia roretzi) body wall muscle." J.Biochem.113. 321-326 (199)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kohama,K.,et al.: "A novel regulatory effect of myosin light chain kinase from smooth muscle on the ATP-dependent interaction between actin and myosin." Biochem.Biophys.Res.Commun.184. 1204-1211 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Nakamura,F.,et al.: "Identification of the regulatory site in smooth muscle calponin that is phosphorylated by protein kinase C." J.Biol.Chem.268. 6194-6201 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Tanokura,M.and Ebashi,S.: "Complexes of myosin subfragment 1 with pyrophosphate and with adenosine diphosphate as studied by phosphorus-1 nuclear magnetic resonance." J.Biochem.113. 19-21 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Masuda,Y.,et al.: "Protease-sensitive anchoring of microfilament bundles provides tracks for cytoplasmic streaming in Vallisneria." Protoplasma. 162. 151-159 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Furuhashi,K.,et al.: "Phoshporylation by actin kinase of pointed end-domain on the actin molecule." J.Biol.Chem.297. 9326-9330 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Higashi-Fujime,S.: "Reconstitution of active movement in vitro based on the actin-myosin interaction." Int.Rev.Cytl.125. 95-138 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kazuhiro Kohama: "Calcium Inhibition" Japan Sci.Soc.Press,and CRC Press., 205 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kazuhiro Kohama,Ryoki Ishikawa,and Tsuyoshi Okagaki: "Calcium Inhibition of Physarum Actomyosin System:Myosin-linked and Actin-linked Natures.In Calcium Inhibition p.p.91-107." Japan Sci.Soc.Press,and CRC Press.K.Kohama,ed., 16 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Masuda, T., et al.: "Confocal laser microscopy of dystrophin localization in quinea pig skeletal muscle fibers." J.Cell Biol.119. 543-548 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Ishikawa, R., et al.: "Stimulation of the interaction between actin and myosin by Physarum caldesmon-like protein and smooth muscle." J.Biol.Chem.266. 21784-21790 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kamitsubo, E., et al.: "Immobilization of endoplasm flowing contiguous to the actin cables upon electrical stimulus in Nitella internodes." Protoplasma. 168. 82-86 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kobayashi, H., et al.: "Isolation of cDNA for 155 kDa protein exhibiting myosin light chain kinase activity." J.Biochem.112. 786-791 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kohno, T., et al.: "Partial purfication of myosin from lily pollen tubes by monitoring with in vitro motility assay." Protplasma. 170. 77-85 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Takagi, T., et al.: "Structure of 29 kDa protein from ascidian(Halocynthia roretzi) body wall muscle." J.Biochem.113. 321-326 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kohama, K., et al.: "A novel regulatory effect of myosin light chain kinase from smooth muscle on the ATP-dependent interaction between actin and myosin." Biochem.Biophys.Res.Commun.184. 1204-1211 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Nakamura, F., et al.: "Identification of the regulatory site in smooth muscle calponin that is phosphorylated by protein kinase C." J.Biol.Chem.268. 6194-6201 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Tanakura, M.and Ebashi, S.: "Complexes of myosin subfragment 1 with pyrophosphate and with adenosine diphosphate as studied by phosphorus 1 nuclear magnetic resonance." J.Biochem.113. 19-21 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Masuda, Y., et al.: "Protease-sensitive anchoring of microfilament bundles provides tracks for cytoplasmic streaming in Vallisneria." Photoplasma. 162. 151-159 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Furuhashi, K., et al.: "Phosphorylation by actin kinase of pointed end-domain on the actin molecule." J.Biol.Chem.297. 9326-9330 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Higashi-Fujime, S.: "Reconstitution of active movement in vitro based on the actin-myosin interaction." Int.Rev.Cytl.125. 95-138 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Yonemura, S., et al.: "Mass isolation of cleavage furrows from dividing sea urchin eggs." J.Cell Sci.100. 73-84 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Igarashi, M., et al.: "Actin-binding proteins in the growth cone particles(GCP) from fetal rat brain : a 44 kDa actin-binding protein is enriched in the fetal GCP fraction." Develop.Brain Res.67. 197-203 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Tosuji, H., et al.: "Calyculin A induces contractile ring-like apparatus formation and condensation of chromosomes in unfertilized sea urchin eggs" Proc.Nat.Acad.Sci.USA. 89. 10613-10617 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kohama, K., et al.: "Calcium-binding proteins that are involved in the calcium inhibition on the actomyoshin system of a lower eukaryote, Physarum polycephalum." Biomed.Res.14 Suppl. 2. 57-62 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Masuda,T.,et al.: "Confocal laser microscopy of dystrophin localization in guinea pig skeletal muscle fibers." J.Cell Biol.119. 543-548 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Ishikawa,R.,et al.: "Stimulation of the interaction between actin and myosin by Physarum caldesmon-like protein and smooth muscle caldesmon." J.Biol.Chem.266. 21784-21790 (1991)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kamitsubo,E.,et al.: "Immobilization of endoplasm flowing contiguous to the actin cables upon electrical stimulus in Nitella internodes." Protoplasma. 168. 82-86 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kobayashi,H.,et al.: "Isolation of cDNA for 155kDa protein exhibiting myosin light chain kinase activity." J.Biochem.112. 786-791 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kohno,T.,et al.: "Partial purfication of myosin from lily pollen tubes by monitoring with in vitro motility assay." Protplasma. 170. 77-85 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Takagi,T.,et al.: "Structure of 29 kDa protein from ascidian(Halocynthia roretzi)body wall muscle." J.Biochem.113. 321-326 (199)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kohama,K.,et al.: "A novel regulatory effect of myosin light chain kinase from smooth muscle on the ATP-dependent interaction between actin and myosin." Biochem.Biophys.Res.Commun.184. 1204-1211 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Nakamura,F.,et al.: "Identification of the regulatory site in smooth muscle calponin that is phosphorylated by protein kinase C." J.Biol.Chem.268. 6194-6201 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Tanokura,M.and Ebashi,S.: "Complexes of myosin subfragment 1 with pyrophosphate and with adenosine diphosphate as studied by phosphorus- 1 nuclear magnetic resonance." J.Biochem.113. 19-21 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Masuda,Y.,et al.: "Protease-sensitive anchoring of microfilament bundles provides tracks for cytoplasmic streaming in Vallisneria." Protoplasma. 162. 151-159 (1991)

    • Related Report
      1993 Annual Research Report
  • [Publications] Furuhashi,K.,et al.: "Phoshporylation by actin kinase of pointed end-domain on the actin molecule." J.Biol.Chem.297. 9326-9330 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Higashi-Fujime,S.: "Reconstitution of active movement in vitro based on the actin-myosin interaction." Int.Rev.Cytl.125. 95-138 (1991)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kazuhiro Kohama: "Calcium Inhibition" Japan Sci.Soc.Press,and CRC Press., 205 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kazuhiro Kohama,Ryoki Ishikawa,and Tsuyoshi Okagaki: "Calcium Inhibition of Physarum Actomyosin System:Myosin-linked and Actin-linked Natures.In Calcium Inhibition p.p.91-107." Japan Sci.Soc.Press,and CRC Press.K.Kohama,ed., 16 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Ishikawa,R.,Kagami,C.,Hayashi,C.and Kohama,K.: "The binding of nonmuscle caldesmon from brain to microtubules.Refulation by Ca^<2+>-caldesmon and cdc 2 kinase." FEBS Lett.299. 54-56 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Kohama,K.,Okagaki,T.,Hayakawa,K.,Lin,Y.,Ishikawa,R.,Shimmen,T.and Inoue,A.: "A novel regulatory effect of myosin light chain kinase from smooth muscle on the ATP-dependent interaction between actin and myosin." Biochem. BioPys. Res. Commun.184. 1204-1221 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Lin,Y.,Ishikawa,R.and Kohama,K.: "A novel regulatory protein that affects the function of caldesmon and myoshin light chain kimase." Biochem. Biophys. Res. Commun.184. 1212-1218 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Ishikawa,R.,Okagaki,T.and Kohama,K.: "Regualtion by Ca^<2+>-calmodulin of the actin-bundling activity of Physarum 210-KDa protein." J. Muscle Res. Cell motil.13. 321-328 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Kohno,T.,Ishikawa,R.,Nagata,T.,Kohama,K.and Shimmen,T.: "Partial Purification of myosin from lily pollen tubes by monitoring with in vitro motility assay." Protoplasma. 170. 77-85 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Ishikawa,R.,Kagami,O.,Hayashi,C.and Kohama,K.: "Characterization of smooth muscle caldesmon as a microtubule-associated protein." cell motil. Cytoskel.23. 244-251 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Kazuhiro Kohama: "Calcium Inhibition" Japan Sci.Soc.Press,and CRC Press., 205 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Kazuhiro Kohama,Ryoki Ishikawa,and Tsuyoshi Okagki: "Calcim Inhibition of Physarum Actomyosin System:Myosin-linked and Actin-linked Natures.In Calcium Inhibition P.P.91-107" Japan Sci.Soc.Press,and CRC Press.K.Kohama,ed., 16 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Kohno,T.,Okagaki,T.,Kohama,K.and Shimmen,T.: "Pollen tube extract supports the movement of actin filaments in vitro." Protoplasma. 161. 75-77 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kohama,K.,Lin,Y.,TakanoーOhmuro,H.and Ishikawa,R.: "A myosinーlike protein from smooth muscle." Journal Cell Science. Suppl.14. 59-61 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Okagaki,T.,HigashiーFujime,S.,Ishikawa,R.,TakanoーOhmuro,H.and Kohama,K.: "In Vitro movement of actin filaments on gizzard smooth muscle myosin:Requirement of phosphorylation of myosin light chain and effects of tropomyosin and caldesmon." Journal Biochemistry. 109. 858-866 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Okagaki,T.,Ishikawa,R.and Kohama,K.: "Purification of a novel Caーbinding protein that inhibits myosin light chain kinase activity in lower eukaryote,Physarum polycephalum." Biochemical Biophysical Research Communication. 176. 564-570 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kohama,K.,Okagaki,T.,TakanoーOhmuro,H.and Ishikawa,R.: "Characterization of calciumーbinding light chain of Ca^<2+> receptive subunit of Physarum myosin." Journal Biochemistry. 110. 566-570 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kohama,K.,Kohno,T.,Okagaki,T.and Shimmen,T.: "Role of actin in the myosinーlinked Ca^<2+>ーregulation of ATPーdependent interaction between actin and myosin of lower eukaryote,Physarum polycephalum." Journal Biochemistry. 110. 508-513 (1991)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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