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Studies of biophysical approaches in cell biology

Research Project

Project/Area Number 03304055
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field 生物物性学
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

OHNISHI Shun-ichi  Kyoto University. Fac. of Science , Professor, 理学部, 教授 (00025272)

Co-Investigator(Kenkyū-buntansha) TAKEICHI Masatoshi  Kyoto Univ. Fac. of Science, Professor., 理学部, 教授 (00025454)
MABUCHI Issei  Tokyo Univ. Dept. of Pure and Applied Science, Professor, 教養学部, 教授 (40012520)
HOTANI Hirokazu  Teikyo Univ. School of Science and Engineering. Professor, 理工学部, 教授 (80025444)
KAMIYA Ritsu  Tokyo Univ. Fac. of Science. Associated Professor, 理学部, 教授 (10124314)
KUSUMI Akihiro  Tokyo Univ. Dept. of Pure and Applied Science, Associated Professor., 教養学部, 助教授 (50169992)
Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1992: ¥3,000,000 (Direct Cost: ¥3,000,000)
Keywordscell biology / biophysical approach / cell shape / nanometer optics / cell adhesion / intracellular vesicular transport / neurobiology / cadherin / 生物物理的アプロ-チ / ナノメ-タ-計測 / ヘミデスモソ-ム
Research Abstract

We had a symposium entitled "Studies of biophysical approaches in cell biology" with the same title as the present Sogo-Kenkyu (A). It was held November 5, 1992 in the annual meeting of the Biophysical Society of Japan. The organizer was Shun-ichi Ohnishi and Akihiro Kusumi. The speakers were Akihiro Kusumi on Introduction: Dynamic structure of cell membranes studied by nanosecond optical microscopy, Kazuhiko Kinoshita on Direct visualization and operation of cellular and molecular processes by a new optical microscopic technique, Hiroshi Miyamoto on STM and AFM observation of biomembranes and cytoskeletons, Masatoshi Takeichi on the Cellular adhesion and morphogenesis by the cytoplasmic factors, Mitsuhiro Yanagida on Signal transduction in chromosome, Masami Mishina on Molecular neurobiology, and Nobutaka Hirokawa on Molecular cell biology of neurons and cytoskeletons.
The early half talks were on mainly biophysical studies and the latter half was on cellular and molecular biological studies. We expect that the biophysical approaches in cell biology will grow sooner and better.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] 鍵和田 聡: "In vitrofusion of rabbit liver Golgi membranes with liposomes" J.Biol.Chem.268. 1430-1435 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 種田 貴徳: "Fluorescence lifetime imaging microscopy(filmscopy)Methodology development and application to studies of endosome fusion in single cells" Biophys.J.64. (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] S.Tanaka: "Three-headed outer armdynein from Chlamy domonas that can functionally combine with outer-arm-missing axonemes." J.Biochem.111. 758-762 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] H.Miyata: "Morphologioal changes in liposomes caused by polymerization of encapsulated actin and spontaneous formation of action bundles" Proc.Natl.Acad.Sce.USA. 89. 11547-11551 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] H.Toduji: "Calyculin A induces contrahtile ring.like apparatus formation and condens actin of chromosomes in unfertilized sea urchin eggs." Proc.Natl.Acad.Sci.USA. 89. 10613-10617 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] S.Hirano: "Identification of a neural α-catenin as a key regular of cadnerin function and Multicellular organization" Cell. 70. 293-301 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 馬淵 一誠: "Advances in Comparafive and Enviromental Physiology" Springer-Verlag Berlin Heidelberg., 33 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] S. Kagiwada: "Invitro fusion of rabbit liver Golgi membranes with liposomes" J. Biol. Chem.268. 1430-1435 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T. Oida: "Fluorescence lifetime imaging microscopy (filmscopy). Methodology development and application to studies of endosome fusion in single cells" Biophys. J.64. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] S. Tanaka: "Three-headed outer armdynein from chlamydomonas that can functionally combine with outer-arm-missing axonemes." J. Biochem. 111. 758-762 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] H. Miyata: "Morphological changes in liposomes caused by polymerization of en capsulated actin and spontaneous formation of actin bundles." Proc. Natl. Acad. Sci. USA. 89. 11547-11551 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] H. Tosuji: "Calyculin A induces contractile ring- like apparatus formation and condens actin of chromosomesin" Proc. Natl. Acad. Sci. USA. 89. 10613-10617 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] S. Hirano: "Identification of a neural alpha-atenin as a key regular of cadherin function and Multicellular organization" Cell. 70. 293-301 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 鍵和田 聡: "In vitro fusin of rabbit liver Golgi membranes with liposomes." J.Biol.Chem.268. 1430-1435 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 種田 貴徳: "Fluorescence lifetime imaging microscopy(filmscopy)Methodology development and application to studies of endosome fusion in single cells." Biophy.J.64. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] S.Tanaka: "THree-headed outer armdynein from chlanydomonas that can functionally combine with outer-arm-missing axonemes." J.Biochem.111. 758-762 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] H.Miyata: "Morphological changes in liposomes caused by polymerization of encapsulated actin and spontaneous for mation of actin bundles." Proc.Natl.Acad.Sci.USA. 89. 11547-11551 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] H.Tosuji: "Calyculin A induces contractile ring-lika apparatus formation and condens actin of chromosomes in unfertilized sea urchin eggs." Proc.Natal.Acad.Sci.USA. 89. 10613-10617 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] S.Hirano: "Identification of a neural α-catenin as a key regular of cadhelin function and Multicellular organization." Cell.70. 293-301 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 馬淵 一誠: "Advances in Comparative and Envirowmental Physiology" Springer-Verlag Berlin Heidelberg, 33 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 村田 昌之: "pHーdependent membrane fusion and vesiculation of phospholipid large unilamellar vesicles induced by amphiphilic anionic and cationic peptide" Biochemistry. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 村田 昌之: "Membrane fusion induced by mutual interaction of the two chargereversed amphiphilic peptides at neutral pH'" J.Biol.Chem.266. 14353-14358 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Sekimura,T.: "The morphnogenesis of liposomes viewed from the aspect of binding energy" J.Theor.Biol.149. 1350-1355 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Hotani,H.: "Morphological changes of liposomes caoused by polymerization of encapsulated actin and spontateneous formation of actin bundles" Proc.Natl.Acad.Sci.U.S.A.(1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 楠見 明弘: "Development of a streakーcameraーbased time resolved microscope fluorimeter and its application to studies of membrane fusion in single cells" Biochemistry. 30. 6517-6527 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 楠見 明弘: "Effect of alkyl chain unstauration and cholesterol intercalation on oxygen transport in membranes: a pulse ESR spin labelling study" Biochemistry. 30. 8578-8590 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Asano,K.: "Membrane Interaction of HIV." New York:WileyーLiss,Inc., 8 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 宝谷 紘一: "暗視野顕微鏡。限界を越える生物顕微鏡ー見えないものを見る" 学会出版センタ-, 15 (1991)

    • Related Report
      1991 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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