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STUDY ON ACTION MECHANISM OF BRASSINOSTEROIDS

Research Project

Project/Area Number 03305009
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field 広領域
Research InstitutionTEIKYO UNIVERSITY

Principal Investigator

YOKOTA Takao  TEIKYO UNIV, DEPT OF BIOSCI, PROFESSOR, 理工学部, 教授 (40011986)

Co-Investigator(Kenkyū-buntansha) TAKEUCHI Yasutomo  UTSUNOMIYA UNIV, WEED SCI RES CENTER, PROFESSOR, 雑草科学センター, 教授 (90008003)
TAKATSUTO Suguru  JOETSU UNIV OF EDUC, DEPT OF CHEM EDUC, ASSOCIATE PROFESSOR, 学校教育学部, 助教授 (30179460)
ABE Hiroshi  TOKYO UNIV OF AGRIC AND TECH, DEPT OF APPLIED BIOL SCI, PROFESSOR, 応用生物科学科, 教授 (10015084)
SAKURAI Akira  INST OF PHYS CHEM RES (RIKEN), CHIEF RESEARCHER, 主任研究員 (10087562)
KATSUMI Masayuki  ICU, DEPT OF EDUCATION, PROFESSOR, 教養学部, 教授 (10052242)
丸茂 晋吾  名古屋大学, 農学部, 教授 (30023394)
Project Period (FY) 1991 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥16,100,000 (Direct Cost: ¥16,100,000)
Fiscal Year 1993: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1992: ¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1991: ¥8,600,000 (Direct Cost: ¥8,600,000)
KeywordsBRASSINOSTEROID / BIOSYNTHESIS / GROWTH / MICROTUBLE / BLUE LIGHT / ANTIBRASSINOSTEROID / CONJUGATE / MEMBRANE STABILITY / brassinosteroid / 伸長生長 / 植物ホルモン / 耐低温ストレス作用 / 木部分化 / 摘果効果 / アンタゴニスト
Research Abstract

1)A biosynthetic pathway of brassinolide (teasterone -> typhasterol -> cstasterone -> brassinolide) is found in both cell cultures and intact plant tissue. 3-Dehydrotesterone was found as an intermediate between teasterone and typhasterol
2)In pollen of Lolium perenne, brassinosteroid is distributed in amyloplast as examined by immunocytochemistry.
3)A myristate ester of teasterone was identified from lily pollen.
4)Biological activity of brassinosteroids in rice leaf was increased by irradiation of blue light which causes unrolling of the rice leaf. However, this is not due to increased level of endogenous brassinosteroids.
An antibrassinolide termed KM-01 was isolated from a fungus Drechslera avenae and the chemical structure was determined.
Slow-releasing synthetic brassinolides were synthesized and a candidate was seltected for practical agricultural use.
7)Conditioning effects of brassinolide on seed germination of Striga and Orobanche were analyzed.
8)In contrast to auxin, growth effect of brassinosteroids is related with microtuble behavior and with inner layr of stem tissue.
9)Cold tolerance effects of brassinosteroids are based on their stabilizing effects of plant membrane.

Report

(4 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • 1991 Annual Research Report
  • Research Products

    (43 results)

All Other

All Publications (43 results)

  • [Publications] T.Iwasaki: "Brassinosteroids act as regulators of tracheary element differentiation in isolated Zinnia mesophyll cells" Plant & Cell Physiology. 32. 1007-1014 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H.Suzuki: "Metabolism of castasterone and brassinolide in mung bean explant" Phytochemistry. 33. 1361-1367 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H.Suzuki: "Biosynthesis of brassinosteroids in Catharanthus roseus.Part I Biosynthesis of brassinolide from castasterone in cultured cells of Catharanthus roseus" J.Plant Growth Regul.12. 101-106 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H.Abe: "Effect of Introducing a lacfone group into typhasterol and teasterone to promote rice eamira inclination" Biosci.Biotech.Biochem.56. 1312-1313 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] R.Tominaga: "Brassinolide-induced elongation of inner tissues of squash(Cucurbit a maxima Duch.)hypocotyl segments" Plant & Cell Physiology. (投稿中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] S.-K.Kim: "Brassinolide-Inhibitor KM-01,its isolation and structure elucidation from a fungus Drechslera avenae" Tetrahedron Letters. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H.G.Cutler: "Brassinosteroids.Chemistry,Bioactivity & Applications" アメリカ化学会, 358 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T Yokota: "Transport and metabolism of brassinosteroids in rice" Progress in Plant Growth Regulation (ed. CM Karssen et al), Kluwer Academic Publishers. 298-305 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H Suzuki: "Metabolism of castasterone and brassinolide in mung bean explant" Phytochemistry. 33. 1361-1367 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H Suzuki: "Biosynthesis of brassinolide from castasterone in cultured cells of Catharanthus roseus" J Plant Growth Regul. 12. 101-106 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H Suzuki: "Biosynthesis of brassinolide from teasterone via typhasterol and castasterone in cultured cells of Catharanthus roseus" J Plant Growth Regul. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H Abe: "Rice lamina inclination, endogenous levels in plant tissues and accumulation during pollen development of brassinosteroids" Brassinosteroids. Chemistry, Bioactivity & Applications (ed. HG Cutler, T Yokota, G Adam). 200-207 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] S Asakawa: "Teasterone 3-myristate : A new type of brassinosteroid derivative in Lilium longiflorum anthers." Biosci Biotech Biochem. 58. 219-220 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H Abe: "Effect of blue light on endogenous brassinosteroid activity and leaf unrolling in rice seedlings" Plant Physiol. (submitted).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] S-K Kim: "A brassinolide-inhibitor KM-01, Its isolation and structure elucidation from a fungaus Drechslera avenae." Tetrahed Lett.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Y Kamuro: "Practical uses of brassinolide derivatives : Promoting effects on feritilization and seed setting" Proceedings of 19th Annual Meeting of Plant Growth Regulator Society of America (San Fransisco, July 17-10). 275-277 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Y Takeuchi: "Effects of brassinolide on conditioning and germination of witchweed (Striga asiatica) seeds." Brassinosteroids. Chemistry, Bioactivity & Applications (ed. HG Cutler, T Yokota, G Adam). 298-305 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Y Takeuchi: "Effects of brassinosteroids on conditioning and germination of clover broomrape (Orobanche minor)seeds." J Plant Growth Regul. (to be submitted).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] S Kuraishi: "Effect of brassinolide on levels of indoleacetic acid and abscisic acid in squash hypocotyls" Brassinosteroids. Chemistry, Bioactivity & Applications (ed. HG Cutler, T Yokota, G Adam). 312-319 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] R Tominaga: "Brassinolide-induced elongation of inner tissues of squash (Cucurbita maxima duch.) hypocotyl segments." Plant Cell Physiol. (submitted).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T Iwasaki: "Brassinosteroids act as regulators of tracheary-elements differentiation in isolated Zinnia mesophyll cells." Plant Cell Physiol. 32. 1007-1014 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M Katsumi: "Brassinolide protects plant tissues from chilling injury" (to be submitted).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M Seki: "Effect of brassinolide on cortical microtuble arrangements under chilling stress." Abstracts of papers, XI International Botanical Congress (Yokohama, Aug 28-Sep 3). 457 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H、Suzuki: "Metabolism of Castasterone and brassinolide in mung bean explaut" Phytochemistry. 33. 1361-1367 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] H、Suzuki: "Biosyn thes is of brassinosteroids in Cathurauthus roseus、Purt I Biosynthesis of brassinolide from castasferone" J、Plant Growth Regnlation. 12. 101-106 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] S、Asakawa: "Teasterone 3-myristate:A New type of brassinosteroid deriuatioe in Lilium Longiflorum nuthers" Biosci Biotech Biochem. 58. 219-220 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] P、E、Taylor: "Detection of brassino steroids in pollen of Lilium Derennel by immunocutocheonistoy" Planta. 189. 91-100 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] S-K Kim: "A brassinolide-inhibitor KM-01,Its iso lation and Structureelucidation from a fungus Drechslera avenae" Tetrahedron Letters. (印刷中).

    • Related Report
      1993 Annual Research Report
  • [Publications] H Suzuki: "Biosynthesis of brassino lide from teasteronevia typhasterol anf castasterone in cultured colls of C.roseus" J.Plant Growth Reguluation. (印刷中).

    • Related Report
      1993 Annual Research Report
  • [Publications] P.E.Taylor: "Detetion of brassinosteroids in pollen of lolium perenne L by mmunocy to Chemisry" Planta.

    • Related Report
      1992 Annual Research Report
  • [Publications] H.Suzuki: "Metabolism of costasterone and brassinolide in nuug bean explant" Phytochemistry.

    • Related Report
      1992 Annual Research Report
  • [Publications] H.Abe: "Effect of Introducing a lactone group into typhasterol and Teasterone to promote rice lamina inclination" Biosci.Biotech.Biochem.56. 1312-1313 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 竹内 安智: "ブラシノステロイドの生理作用と利用に関する研究" 植物の化学調節. 27. 1-10 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Y.kamuro: "Practical uses of brassinolide deriratives:Promoting effects on fertilization and seedsetting" Proceedings of 19th Annual meeting of Plant Groath Regulator Society of America. (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] M.Bohl: "Molecular Structure and Biological Activity of Steroids" CRC press, 471 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] C.M.Karssen: "Progress in plant growth substances" Kluwer Academic Publishers, 963 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] S.Takatsuto: "An lmproved Preparation of Steroidal 2ーEne Compounds from the corresponding 3βーTosylates" Biosci.Biotech.Biochem. 56. 163-164 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] T.Iwasaki: "Brassinosteroids act a regnlators of tracheary element diffentiation in isolated Zinnia mesophyll cells" Plant Cell Physiol.32. 1007-1014 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] T.Ando: " _<13>C NMR assignment of brassinosteroids by tiooーdimentional technizues" Magn.Reson.Chem.

    • Related Report
      1991 Annual Research Report
  • [Publications] H.Abe: "Promofive effect of introduction of lactone group in to typhasterol and teusterone on rice lamina imlimation" Biosu,Biofech.Biochem.

    • Related Report
      1991 Annual Research Report
  • [Publications] 竹内 安智: "ブラシノステロイドの生理作用と利用に関する研究" 植物の化学調節. 27. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] H.Suzuki: "Conversion of custasterone to brassinolide im cultured cells and seedlings of Catharanthus roseus" Plant Cell Physiol.

    • Related Report
      1991 Annual Research Report
  • [Publications] H.G.Cutler: "Brassinosteroids,Chemistry,Bioactivity and Applications" American ChemicalSociety, 358 (1991)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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