| Project/Area Number |
03404003
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| Research Category |
Grant-in-Aid for General Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
植物生理学
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| Research Institution | Nagoya University |
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Principal Investigator |
SUGIURA Masahiro Nagoya U.Ctr Gene Res.Prof., 遺伝子実験施設, 教授 (80027044)
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| Co-Investigator(Kenkyū-buntansha) |
HIROSE Tetsuro Nagoya U.Ctr Gene Res.DC, 特別研究員
MATSUBAYASHI Toru Nagoya U.Ctr Gene Res.PD, 特別研究員 (50222310)
WAKASUGI Tatsuya Nagoya U.Ctr Gene Res.Res Assoc, 遺伝子実験施設, 助手 (10212317)
SUGITA Mamoru Nagoya U.Ctr Gene Res.Assoc Prof, 遺伝子実験施設, 助教授 (70154474)
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| Project Period (FY) |
1991 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥30,500,000 (Direct Cost: ¥30,500,000)
Fiscal Year 1994: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1993: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1992: ¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1991: ¥22,600,000 (Direct Cost: ¥22,600,000)
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| Keywords | tobacco / plastid / chloroplast / RNA-binding protein / RNA-binding domain / gene / intron / RNA結合蛋白 / 前駆体RNA / in vitro 翻訳系 / 光合成 / RNAエディティング / プロモーター / オルタナティブ・スプライシング / TATAボックス / 伸長因子EF-Tu / リボソーム蛋白質L28 / 転写後調節 / エキソン |
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| Research Abstract |
Five RNA-binding proteins (RNPs) have been isolated from tobacco chloroplasts, and their cDNAs and genes were analyzed. All five RNPs contain each a transit-peptide at the N-terminus, an acidic domain, and two RNA-binding domains towards the C-terminus. Their genes consist of four exons and three introns, the position of which is identical among the five genes.
All five RNPs, though showing strong affinity to poly (G) and poly (U), bind to all plastid RNAs examined except mature tRNAs, suggesting little RNA sequence specificity. After removing all RNPs by their antibodies from chloroplast lysates, plastid RNA processing and translation activities increased. This result suggests that chloroplast RNPs function to protect and store RNAs, or to partition plastid transcription and translation systems.
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