| Project/Area Number |
03404012
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (A)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
基礎獣医学
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
OGAWA Tomoya Univ.Tokyo, Fac.Agri., Professor, 農学部, 教授 (30087572)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
HAYASHI Yoshihiro Univ.Tokyo, Fac.Agri., Professor, 農学部, 教授 (90092303)
NAKAHARA Yuko Inst.Phys.&Chem.Res., Senior Res.Scientis Lab.Synth.Cell.Chem.,, 細胞制御化学研究室, 主任研究員
TAKAHASHI Michio Univ.Tokyo, Fac.Agri., Professor, 農学部, 教授 (30011943)
SHIOTA Kunio Univ.Tokyo, Fac.Agri., AssociateProf., 農学部, 助教授 (80196352)
能田 健 東京大学, 農学部, 助手 (60218287)
伊藤 喜久治 東京大学, 農学部, 助教授 (50100045)
|
|---|
| Project Period (FY) |
1991 – 1994
|
| Project Status |
Completed (Fiscal Year 1994)
|
| Budget Amount *help |
¥29,100,000 (Direct Cost: ¥29,100,000)
Fiscal Year 1994: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1993: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1992: ¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 1991: ¥20,400,000 (Direct Cost: ¥20,400,000)
|
|---|
| Keywords | Oligosaccharide / Glycoconjugate / Carbohydrate Epitope / Proteoglycan / Glycoprotein / Glycolipids / Placental Lactogen / Equine Chorionic Gonadotropin / GPIアンカー / スフィンゴ糖脂質 / PL-Im / 絨毛性性腺刺激ホルモン / ガングリオシド / Rcho-1 / N-結合型糖鎖 / クロボ系糖脂質 / 胎盤性ラクトジエン / PMSG / 下垂体膜タンパク質 / Rcho-1細胞 / hCG / トリパノソーマ・ブルセイ / グロボ系糖脂質 / rPL-1 / ピリジルアミン / Nー結合型糖鎖 / トリパノソ-マ・ブルセイ / GPIアンカ- / rPLー1 |
|---|
| Research Abstract |
Oligosaccharides in biological systems are usually conjugated to lipid or proteins. It is as glycoconjugates that are found in the cytoplasm, on cell membranes and in extra-cellular fluids and matrices. The ability of accurately sequence the oligosaccharide units of glycoconjugates has revealed a remarkable complexity and diversity of these molecules. However, despite some observations suggesting important biological roles for these sugar chains, a single common theory has not emerged to explain this diversity. In this research project, we have studied the biological roles of glycoconjugates by various approach including organic synthesis of oligosaccharides (carbohydrate epitopes of proteoglycans, glycosyl phosphatidylinositol, carbohydrate epitopes of glycoprotein, glycolipids), cDNA cloning (rat placental lactogen, equine chorionic gonadotropin) and production of recombinant glycoproteins, analysis of glycolipids (placental gangliosides) which linked to biological phenomena and morphological approach using lectin- or immuno-staining (mouse placental trophoblast-cells, goat testicular cells).
|
