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Primary brain lymphoma : analysis of gene alteration

Research Project

Project/Area Number 03404044
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field Cerebral neurosurgery
Research InstitutionNiigata University

Principal Investigator

KUMANISHI Toshiro  Niigata University, Brain Res Inst, Professor, 脳研究所, 教授 (40018601)

Co-Investigator(Kenkyū-buntansha) USUI Hiroshi  Niigata University, Brain Res Inst, Assistant Researcher, 脳研究所, 助手 (20192510)
WASHIYAMA Kazuo  Niigata University, Brain Res Inst, Associate Professor, 脳研究所, 助教授 (00183715)
ABE Satoshi  Niigata University, Brain Res Inst, Assistant Researcher, 脳研究所, 助手 (90202663)
桑野 良三  新潟大学, 遺伝子実験施設, 助教授 (20111734)
Project Period (FY) 1991 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥29,600,000 (Direct Cost: ¥29,600,000)
Fiscal Year 1994: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1993: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1992: ¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1991: ¥17,600,000 (Direct Cost: ¥17,600,000)
KeywordsMalignant Lymphoma / Brain Tumor / Non-Hodgkin Lymphoma / Immunoglobulin / Cene Rearrangement / Tumor Suppressor Gene / p53遺伝子
Research Abstract

The objective of this study was to analyze gene alterations of the brain lymphomas and, based on the results which might be specific for a given individual tumor, to investigate the original cells of this tumor. For this sake, cloning and nucleotide analysis of the rearranged regions of the immunoglobulin genes were performed, first. In this case, however, since we used tumor tissue samples, it was required to compare various cloning techniques and to establish and use the most suitable technique to avoid a possibility that we might clone DNA from reactive lymphocytes which coexist frequently in neoplastic tissues. After the comparison of various cloning techniques, we found that a combination of successive procedures consisting of electrophoretic fractionation of restriction enzyme-digested genomic DNA,PCR-amplification, cloning and comparison of clones among fractions was most simple, rapid and reliable. Actually, we have determined nucleotide sequences of rearranged regions of immun … More oglibulin genes in a number of brain lymphoma cases by this approach (manuscript in preparation). The nucleotide sequences obtained were useful to eaxamine whether or not the neoplastic cells were derived from the same orgin and also to search whether or not the same cells were present, if not proliferating, in generalized lymphatic apparatus. In this study, we also examined tumor suppressor genes in brain lymphomas and detected p53 mutations in 60-80%, 15 and p16 gene mutations in 60-80% of cases, but p21 gene mutation in none of cases examined. These mutation were compared with those in various brain tumors other than brain lymphomas. In addition, we have cloned human gene of 2', 3'-cyclic nucleotide 3'-phosphodiesterase which was expressed in oligodendrocytes and also in lymphocytes and determined the gene structure and its chromosomal localization. Attempt to establish brain lymphoma cell lines was also performed and obtained 2 cell lines and immunoglobulin genes of which were examined by the same method described. Less

Report

(5 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • 1992 Annual Research Report
  • 1991 Annual Research Report
  • Research Products

    (59 results)

All Other

All Publications (59 results)

  • [Publications] 阿部 聰: "Intravascular malignant lymphomatosis-いわゆる neoplastic angioendotheliosis-." 神経内科. 34. 154-159 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Monoh,K.: "Structure,expression and chromosomal localization of the gene encoding human 2',3'-cyclic nucleotide 3'-phosphodiesterase." Gene. 129. 297-301 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Zhang,S.: "P53 gene mutations in pontine gliomas of juvenile onset." Biochem.Biophys.Res.Commun.196. 851-857 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 藤田登志也: "頭蓋内B細胞悪性リンパ腫細胞株:YMF." Human Cell. 6. 314-315 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Koga,H.: "Analysis of P53 gene mutations in low and high grade astrocytomas by PCR-SSCP and immunohistochemistry." Acta Neuropathol.87. 225-232 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Motoyama,T.: "Functional and morphological effects of tumour necrosis factor α in an interleukin 6-producing pulmonary large cell carcinoma with sarcomatoid features." Virchows Arch. 424. 361-366 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawasaki,T.: "mRNA and protein expression of p53 mutations in human bladder cancer cell lines." Cancer Lett.82. 113-121 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Koga,H.: "Primary malignant lymphoma of the brain:demonstration of the p53 gene mutations by PCR-SSCP analysis and immunohistochemistry." Brain Tumor Pathol.11. 151-155 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hayakawa,T.: "Primary central nervous system lymphoma in Japan-A retrospective,co-operative study by CNS-Lymphoma Study Group in Japan-." J.Neuro-Oncol.19. 197-215 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Zhang,SJ.: "Analysis of p53 gene mutations in pontine gliomas of juvenile onset." Brain Pathol.4. 425- (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Koga,H.: "p53 gene mutations in primary malignant lymphoma of the brain." Brain Pathol.4. 447- (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Feng,X.: "Intracranial germ cell tumors:Detection of p53 gene mutations by single-strand conformation polymorphism (SSCP) analysis." Jap.J.Cancer Res.86(in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Koga,H.: "Analysis p53 gene mutations in human glioma cell lines." Brain Tumor Pathol.(in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kobayashi,T.: "Molecular cloning of cDNAs for Immunoglobulin variable regions of a monoclonal anti-idiotypic antibody specific for sigma receptors." J.Neuroimmunol.(in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tsumanuma,I.: "The analysis of p53 tumor suppressor gene in pineal parenchymal tumors." Brain Tumor Pathol.(in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Fujita,T.: "In vitro transformation of primary human B cell lymphoma cell of the brain into monocyte/macrophage." Cancer. (in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 熊西敏郎: "主題"分子生物学の脳腫瘍病理への貢献":脳原発悪性リンパ腫;P16(MTS)がん抑制遺伝子変異の検出。" 第13回日本脳腫瘍病理研究会(熊本). 発表予定. (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 阿部 聰: "シンポジウム「Intravascular malignant lymphomatosis の病態」:IMLにおける腫瘍細胞の解析." 第36回日本神経学会総会(名古屋). 発表予定. (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 鷲山和雄: "ヒトがん細胞株とその特性:脳腫瘍" 中外医学社(東京), 472 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] CNS Lymphoma Study Group: "中枢神経系悪性リンパ腫" 篠原出版(東京), 217 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Abe, S.: "Intravascular malignant lymphomatosis-So-called neoplastic angioendotheliosis-. (in Japanese)" Neurological Medicine. 34. 154-159 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Washiyama, K.: "Human cancer cell lines and their characteristics : brain tumor. (in Japanese)" Suzuki, T., Sekiguchi, M., Nozawa, S.(eds.), Chugai-Igaku (Tokyo). (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Monoh, K.: "Structure, expression and chromosomal localization of the gene encoding human 2', 3'-cyclic nucleotide 3'-phosphodiesterase." Gene. 129. 297-301 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Zhang, S.: "p53 gene mutations in pontine gliomas of juvenile onset." Biochem Biophys Res Commun. 196 (2). 851-857 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Fujita, T.: "Intracranial B cell malignant lymphoma cell line : YMF.(in Japanese)" Human Cell. 6 (4). 314-315 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Koga, H.: "Analysis of p53 gene mutations in low and high grade astrocytomas by PCR-SSCP and immunohistochemistry." Acta Neuropathol. 87 (3). 225-232 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Motoyama, T.: "Functional and morphological effects of tumour necrosis factor a in an interleukin 6-producing pulmonary large cell carcinoma with sarcomatoid features." Virchows Arch. 424. 361-366 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] CNS Lymphoma Study Group: "CNS Lymphoma. (in Japanese)" Takakura, K., Yamaura, A.(eds.), Shinohara (Tokyo). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawasaki, T.: "mRNA and protein expression of p53 mutations in human bladder cancer cell lines." Cancer Lett. 82. 113-121 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Koga, H.: "Primary malignant lymphoma of the brain : demonstration of the p53 gene mutations by PCR-SSCP analysis and immunohistochemistry." Brain Tumor Pathol. 11 (2). 151-155 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hayakawa, T.: "Primary central nervous system lymphoma in Japan. -A retrospective, co-operative study by CNS-Lymphoma Study Group in Japan-." J Neuro-Oncol. 19. 197-215 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Zhang, SJ.: "Analysis of p53 gene mutations in pontine gliomas of juvenile onset." Brain Pathol. 4. 425 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Koga, H.: "p53 gene mutations in primary malignant lymphoma of the brain." Brain Pathol. 4. 447 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Feng, X.: "Intracranial germ cell tumors : Detection of p53 gene mutations by single-stand conformation polymorphism (SSCP) analysis." Jap J Cancer Res.(in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Koga, H.: "Analysis of p53 gene mutations and their mRNA and protein expressions in human glioma cell lines." Brain Tumor Pathol. (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kobayashi, T.: "Molecular cloning of cDNAs for immunoglobulin variable regions of a monoclonal anti-idiotypic antibody specific for sigma receptors." J Neuroimmunol. (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tsumanuma, I.: "The analysis of p53 tumor suppressor gene in pineal parenchymal tumors." Brain Tumor Pathol. (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Fujita, T.: "In vitro transformation of primary human B cell lymphoma cell of the brain into monocyte/macrophage." Cancer. (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kumanishi, T.: "Primary malignant lymphoma of the brain ; Mutation of p16 (MTS-1) tumor suppressor gene. Symposium of brain tumor pathology. (in Japanese)" 13th Japanese Meeting of Brain Tumor Pathology (Kumamoto). Abstract : 42. (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Abe, S.: "Molecular genetic analysis of IML cells.Symposium "Pathology of intravascular malignant lymphomatosis". (in Japanese)" The 36th Annual Meeting of the Japanese Society of Neurology (Nagoya). Abtract. (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Koga,H.: "Primary malignant lymphoma of the brain:demonstration of the p53 gene mutations by PCR-SSCP analysis and immunohistochemistry." Brain Tumor Pathol.11. 151-155 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 藤田登志也: "頭蓋内B細胞悪性リンパ腫細胞株:YMF" Human Cell. 6. 314-315 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Hayakawa,T.: "Primary central nervous system lymphoma in Japan-A retrospective,co-operative study by CNS-Lymphoma Study Group in Japan." J.Neuro-Oncol.19. 197-215 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kaga,H.: "p53 gene mutations in primary malignant lymphoma of the Brain." Brain Tumor Pathol.4. 407 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 熊西敏郎: "脳腫瘍におけるp53癌抑制遺伝子変異" 第53回日本癌学会抄録,ワークショップ. 32 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 熊西敏郎: "脳原発悪性リンパ腫:p16(MTS-1)がん抑制遺伝子変異の検出" 第13回日本脳腫瘍病理研究会抄録. 42 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] CNS Lymphoma Study Group: "中枢神経系悪性リンパ腫" 高倉公朋監修,山浦 晶編集.篠原出版(東京), 217 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 藤田登志也: "ヌードマウス継代移植可能な頭蓋内B細胞悪性リンパ腫培養細胞株の樹立." Human Cell. 6(3)(Suppl). 145 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 早川徹: "本邦中枢神経系悪性リンパ腫疫学調査-多施設協同研究結果報告-" 脳腫瘍病理. 10(Suppl). 25 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 古閑比佐志: "脳原発悪性リンパ腫におけるp53癌抑制遺伝子の変異." 脳腫瘍病理. 10(Suppl). 27 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Koga,H.: "Primary malignant lymphoma of the brain:demonstration of the p53 genemutations by PCR-SSCP analysis and immunohistochemistry." Brain Tumor Pathol.(印刷中).

    • Related Report
      1993 Annual Research Report
  • [Publications] 藤田登志也: "頭蓋内B細胞悪性リンパ腫細胞株:YMF." Human Cell. 6(4). 314-315 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Hayakawa,T.: "Primary central nervous system lymphoma in Japan-A retrospective,co-operative study by CNS-Lymphoma Study Group in Japan-." J.Neuro-Oncol.(印刷中).

    • Related Report
      1993 Annual Research Report
  • [Publications] 長嶋 和郎: "CNS-Lymphomaの病理" 脳腫瘍病理,発表予定. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 古閑 比佐志: "グリオーマ及び脳原発悪性リンパ腫におけるp53遺伝子店突然変異の解析" 第一回日本脳腫瘍カンファレンス抄録. 55 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 古閑 比佐志: "脳原発悪性リンパ腫におけるp53がん抑制遺伝子の関与" 脳腫瘍病理,発表予定. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 桃生 勝巳: "ヒト2',3'-cyclic nucleotide3'-phosphodiesterase遺伝子の構造と染色体局在" 神経化学. 31. 130-131 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Monoh,K.: "Structure and chromosomal localization of human 2',3'-cyclic nucleotide 3'-phosphodiesterase gene" Gene,. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Fujuta,Toshiya: "In Vitro Transformation of Primary Human B Cell Lymphoma Cell of the Brain into Monocyte/Macrophage" Cancer.

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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