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Molecular mechanisms for the regulation of sexual development in response to the external signals in fission yeast

Research Project

Project/Area Number 03404072
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field 分子遺伝学・分子生理学
Research InstitutionThe University of Tokyo

Principal Investigator

YAMAMOTO Masayuki  The University of Tokyo Graduate School of Science Professor, 大学院・理学系研究科, 教授 (40114706)

Co-Investigator(Kenkyū-buntansha) WATANABE Yoshinori  The University of Tokyo Graduate School of Science Assistant, 大学院・理学系研究科, 助手 (20212326)
IINO Yuichi  The University of Tokyo Graduate School of Science Lecturer, 大学院・理学系研究科, 講師 (40192471)
Project Period (FY) 1991 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥26,900,000 (Direct Cost: ¥26,900,000)
Fiscal Year 1994: ¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1993: ¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1992: ¥8,300,000 (Direct Cost: ¥8,300,000)
Fiscal Year 1991: ¥12,000,000 (Direct Cost: ¥12,000,000)
Keywordsfission yeast / cAMP / A-kinase / meiosis / RNA / RNA-binding protein / 有性生殖 / 接合 / 接合フェロモン / フェロモン受容体 / 情報伝達 / 栄養源 / 性的分化 / アデニル酸シクラーゼ / Gタンパク質 / 接合フェロモン受容体 / 転写因子 / HMG / DNA結合タンパク質
Research Abstract

The fission yeast cells initiate either a new round of the cell cycle or sexual development depending on the nutritional conditions. When haploid cells mate, they communicate by secreting and accepting the mating pheromones.
Elucidation of the molecular mechanisms underlying these signal transduction systems and sexual differentiation will add much to the field of cell biology.
This project was performed to this purpose. The major results obtained are summarized as follows. 1. We cloned the fission yeast gene for the catalytic subunit of protein kinase A.Analysis using this clone confirmed that cAMP serves as a second messenger that mediates the signal from the nutritional conditions. 2. We showed that the mei2 gene product, which is a key factor essential for the initiation of meiosis in fission yeast, is an RNA-binding protein. Mei2 protein turned out to be required not only prior to premeiotic DNA synthesis but also prior to meiotic division I.A novel RNA species (meiRNA) that cooperates with Mei2 to promote meiotic division I has been identified and characterized. 3. We cloned a number of genes from a plant Arabidopsis thaliana that can transcomplement fission yeast mutants defective in mating and/or meiosis. Whether these genes are involved in control of sexual development in the plant is currently under study. Screening for animal genes that transcomplement fission yeast mutants is also in progress.

Report

(5 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • 1992 Annual Research Report
  • 1991 Annual Research Report
  • Research Products

    (33 results)

All Other

All Publications (33 results)

  • [Publications] Watanabe,Y.: "S.pombe mei2^+ encodes an RNA-binding protein essential for premeiotic DNA synthesis and meiosis I,which cooperates with a novel RNA species meiRNA." Cell. 78. 487-498 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Nocero,M: "Glucose repression of fbp1 transcription in Schizosaccharomyces pombe is partiallyregulated by adenylate cyclase activation by a G protein α subunit encoded by gpa2(git8)" Genetics. 138. 39-45 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hughes,D.: "Transcriptional regulation of a Res nucleotide-exchange factor gene by extracellular signals in fission yeast." J.Cell Science. 107. 3635-3642 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kuromori,T.: "Cloning of cDNAs from Arabidopsis thaliana that encode putative protein phosphatase 2C and a human Dr1-like protein by transformation of a fission yeast mutant." Nucleic Acids Research. 22. 5296-5301 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Davey,J.: "Isolation and characterization of krp,a dibasic endopeptidase required for cell viability in the fission yeast Schizosaccharomyces pombe." EMBOJ.13. 5910-5921 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kunitomo,H.: "Schizosaccharomyces pombe pac2^+ controls the onset of sexual development via a pathway irdependent of the cAMP cascade." Current Genetics. (印刷中). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Watanabe, Y., and Yamamoto, M.: "S.pombe mei2^+ encodes an RNA-binding protein essential for premeiotic DNA synthesis and meiosis I,which cooperates with a novel RNA species meiRNA." Cell. 78. 487-498 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Nocero, M., Isshiki, T., Yamamoto, M., and Hoffman, C.S.: "Glucose repression of fbp1 transcription in Schizosaccharomyces pombe is partially regulated by adenylate cyclase activation by a G protein alpha subunit encoded by gpa2 (git8)." Genetics. 138. 39-45 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hughes, D.A., Yabana, N., and Yamamoto, M.: "Transcriptional regulation of a Ras nucleotide-exchange factor gene by extracellular signals in fission yeast." J.Cell Science. 107. 3635-3642 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kuromori, T., and Yamamoto, M.: "Cloning of cDNAs from Arabidopsis thaliana that encode putative protein phosphatase 2C and a human Dr1-like protein by transformation of a fission yeast mutant." Nucleic Acids Research. 22. 5296-5301 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Davey, J., Davis, K., Imai, Y., Yamamoto, M., and Matthews, G.: "Isolation and characterization of krp, a dibasic endopeptidase required for cell viability in the fission yeast Schizosaccharomyces pombe." EMBO J.13. 5910-5921 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kunitomo, H., Sugimoto, A., Wilkinson, C.R.M., and Yamamoto, M.: "Schizosaccharomyces pombe pac2^+ controls the onset of sexual development via a pathway independent of the cAMP cascade." Current Genetics. (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Watanabe,Y.: "S.pombe mei2^+ encodes an RNA-binding protein essential for premeiotic DNA synthesis and meiosis I,which cooperates with a novel RNA species meiRNA." Cell. 78. 487-498 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Nocero,M: "Glucose repression of fbp1 transcription in Schizosaccharomyces pombe is partially regulated by adenylate cyclase activation by a G protein α subunit encoded by gpa2(git8)" Genetics. 138. 39-45 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Hughes,D.: "Transcriptional regulation of a Ras nucleotide-exchange factor gene by extracellular signals in fission yeast." J.Cell Science. 107. 3635-3642 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kuromori,T.: "Cloning of cDNAs from Arabidopsis thaliana that encode putative protein phosphatase 2C and a human Dr1-like protein by transformation of a fission yeast mutant." Nucleic Acids Research. 22. 5296-5301 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Davey,J.: "Isolation and characterization of krp,a dibasic endopeptidase required for cell viability in the fission yeast Schizosaccharomyces pombe." EMBO J.13. 5910-5921 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kunitomo,H.: "Schizosaccharomyces pombe pac2^+ controls the onset of sexual development via a pathway independent of the cAMP cascade." Current Genetics,. (印刷中). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kayoko Tanaka: "Schizosaccharomyces pombe map3+ encodes the putative M-factor receptor." Molecular and Cellular Biology. 13. 80-88 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Satashi Tabata: "Evidence of meiosis specific regulation of gene expression in lily microsporocytes." Plant Science. 89. 31-41 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yukiko Gotoh: "Schizosaccharomyces pombe Spkl is a tyrosine-phosphorylated protein functionally related to Xenopus MAP kinase." Molecular and Cellular Biology. 13. 6427-6434 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Tatsuya Maeda: "Cloning of the pkal gene encoding the catalytic subunit of the cAMP-dependent protein kinase in Schiz.pombe." Journal of Biological Chemistry. (印刷中). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yoshiyuki Imai: "The fission yeast mating pheromone P-factor" Genes & Development. (印刷中). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yoshiyuki imai: "S.pombe sxa1^+ and sxa2^+ encode putative proteases involved in the mating response." Molecular and Cellular Biology. 12. 1827-1834 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Nobuyoshi Mochizuki: "Reduction in the intracellular cAMP level triggers initiation of sexual development in fission yeast." Mlecular and General Genetics. 233. 17-24 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Takako Isshiki: "Characterization of a fission yeast gene. gpa2. that encodes a Gα subunit involved in the monitoring of nutrition." Genes & Deveiopment. 6. 2455-2462 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Kayoko Tanaka: "Schizosaccharomyces pombe map3^+ encodes the putative M-factor receptor." Molecular and Cellular Biology. 13. 80-88 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Yuichi IINO: "S.pombe pacl^+,whose overxpression inhibits sexual development,encodes a ribonuclease IIIーlike RNase." EMBO Journal. 10. 221-226 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Yoshiyuki IMAI: "Identification of a GtPaseーactivating homolog in Schizosaccharomyces pombe." Mol.Cell.Biol.11. 3088-3094 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Tomoko OBARA: "Isolation and characterization of a gene encoding a Gーprotein α subunit from Schizosaccharomyces pombe:Involvement in mating and sporulation pathways." Proc.Natl.Acad.Sci.USA.88. 5877-5881 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Sanae MIYAKE: "Mapping of four ras superfamily genes by physical and genetic means in Schizosaccharomyces pombe." Curr.Genet.20. 277-281 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Asako SUGIMOTO: "Schizosaccharomyces pombe stell^+ encodes a transcription factor with an HMG motif that is a critical regulator of sexual develpoment." Genes & Development. 5. 1990-1999 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Yoshiyuki IMAI: "S.pobe sxal^+ and sxa2^+ encode putative proteases involved in the mating response." Mol.Cell.Biol.12. (1992)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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