| Project/Area Number |
03452306
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
生物物性学
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| Research Institution | INSTITUTE OF SPACE AND ASTRONAUTICAL SCIENCE |
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Principal Investigator |
SHIMIZU Mikio INSTITUTION OF SPACE AND ASTRONAUTICAL SCIENCE,PROFESSOR, 教授 (90017179)
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| Co-Investigator(Kenkyū-buntansha) |
HIMENO Hyouta IBID,RESEARCH ASSOCIATE, 助手 (80208785)
HASEGAWA Tsunemi IBID,ASSOCIATE PROFESSOR, 助教授 (60095023)
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| Project Period (FY) |
1991 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥6,100,000 (Direct Cost: ¥6,100,000)
Fiscal Year 1993: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1992: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1991: ¥3,700,000 (Direct Cost: ¥3,700,000)
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| Keywords | RNA / amino acid / translation / tRNA identity / tRNA / アミノアシルtRNA合成酸素 / 遺伝暗号 / アミノアシレーション / アミノアシレ-ション / アミノアシルtRNA合成酵素 |
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| Research Abstract |
To discuss the origin of genetic code, the possibility of a specific interaction between RNA and amino acid was studied. Identity determinants of 20 kinds of tRNAs in E.coli are at first investigated, constructing various tRNA mutant transcripts by using the T7 RNA polymerase. Anticodon bases and discriminator nucleotide are found to be the important determinant in most of tRNAs. Consequently, a small RAN composed of anticodon arm and acceptor stem was constructed and its aminoacylation activity was studied enzymatically. Only minihelix or microhelix in some cases works well. Next time, more active amino acids should be used for this type of investigations.
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