| Project/Area Number |
03453114
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
高分子合成
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| Research Institution | Tohoku University |
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Principal Investigator |
KOBAYASHI Shiro Tohoku Univ.,Dept. of Molecular Chem. and Engin., Professor, 工学部, 教授 (10026198)
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| Co-Investigator(Kenkyū-buntansha) |
UYAMA Hiroshi Tohoku Univ.,Dept. of Molecular Chem. and Engin., Research Associate, 工学部, 助手 (70203594)
SHODA Shin-ichiro Tohoku Univ.,Dept. of Molecular Chem. and Engin., Associate Professor, 工学部, 助教授 (10143364)
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| Project Period (FY) |
1991 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥6,200,000 (Direct Cost: ¥6,200,000)
Fiscal Year 1992: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1991: ¥3,700,000 (Direct Cost: ¥3,700,000)
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| Keywords | Enzymatic Polymerization / Polysaccharide / Oligosaccharide / Cellulase / Cellooligosaccharide / alpha-D-Maltosyl Fluoride / Pullulanase / プルラナーゼ / セルロ-ス / セルラ-ゼ / フッ化βーDーセロビオシル / マルトオリゴ糖 / アミラ-ゼ / フッ化αーDーマルトシル |
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| Research Abstract |
The in vitro synthesis of cellulose via a nonbiosynthetic path has been achieved for the first time by condensation of beta-D-cellobiosyl fluoride as substrate for cellulase in a mixed solvent of acetonitrile/acetate buffer (pH 5,5:1)(enzymatic polymerization). Under reaction condition of a higher substrate concentration or higher acetonitrile concentration, cellooligosaccharides were produced predominantly. The present method of using a glycosyl fluoride as substrate for a hydrolytic enzyme in an organic/water mixed solvent has successfully been applied to synthesis of maltooligosaccharides and unnatural oligosaccharides.
A new methodology for regio and stereoselective synthesis of cellooligosaccharide derivatives has been developed by utilizing the following two enzymatic reactions : A cellulase-catalyzed stereoselective lactosylation of an alkyl cellobioside giving rise to a new oligosaccharide having a D-galactose unit at the nonreducing end and a beta-galactosidase-catalyzed regioselective cleavage of the terminal D-galactose unit of the product.
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