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Computer Control of Biodegradable Polymer Production Process by Alcaligenes eutrophus

Research Project

Project/Area Number 03453125
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 化学工学
Research InstitutionOsaka University

Principal Investigator

SUGA Ken-ichi  Osaka, University, Faculty of Engineering, Professor, 工学部, 教授 (20029250)

Co-Investigator(Kenkyū-buntansha) OMASA Takeshi  Osaka, University, Faculty of Engineering, Assistant Professor, 工学部, 助手 (00252586)
SHIMIZU Hiroshi  Osaka, University, Faculty of Engineering, Assistant Professor, 工学部, 助手 (00226250)
SHIOYA Suteaki  Osaka University, Faculty of Engineering, Professor, 工学部, 教授 (50026259)
Project Period (FY) 1991 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1993: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1992: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywordsbiodegradable polymer / PHB / P(HB-co-HV) / molecular weight distribution / mole fraction in copolymer / NMR / control of substrate concentration / P(HB-HV)共重合物 / 一次マルコフ鎖過程 / 生分解性ポリマ- / ポリヒドロキシ酪酸 / 残存生物量 / 高分子重合度 / GPC
Research Abstract

Kinetics of poly(-D(-)-3-hydroxyalkanoate) (PHA) production were studied in Alcaligenes eutrophus H16 (ATCC 17699) fed-batch culture using butyric acid and valeric acid as carbon souces. In poly(-D(-)-3-hydroxybutyric acid) (PHB) production, at the conditions of 3g/l butyric acid concentration and pH 8.0, the maximum values of specific production rate of PHB, production yield, and PHB content in the cell were obtained. Effect of cultivation condition on molecular weight distribution of PHB was studied. The average of molecular weight was decreased as the butyric acid concentration was increased. When the nitrogen source was added to the fermentation medium after adequate PHB accumulation in the cell, not only PHB production but also degradation were simultaneously occurred. The degradation rate of low molecular weight polymer was higher than that of high molecular weight polymer, and as a result, the average of molecular weight distribution shifted to the higher value. By addition of valeric acid to the butyric acid as carbon sources, copolyester of 3-hydroxybutyric acid (3HB) and 3-hydroxyvaleric acid (3HV), which is abbreviated as P(3HB-co-3HV), was produced but the specific production rate was lower than that of PHB.The mole fraction of P(3HB-co-3HV), was also investigated, using butyric acid and valeric acid as carbon sources. The mole fraction can be controlled within a range of compositions (0-40 mole% 3HV) by the mole ratio of butyric acid and valeric acid in the feeding mediums.

Report

(4 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • 1991 Annual Research Report
  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] H.Shimizu,S.Sonoo,S.Shioya,K.Suga: "Production of poly-3-hydroxy butyric acid(PHB) by Alcaligenes eutrophus H16 in a fed-batch culture" Proc.of Asia-Pacific Biochemical Engineering Conference 1992,Yokohama. 195-197 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H.Shimizu,S.Tamura,S.Shioya,K.Suga: "Kinetic study of poly-D(-)-3-hydroxy butyric acid(PHB) production and its molecular weight control in a fed-batch culture of Alcaligenes eutrophus." Journal of Fermentation and Bioengineering. 76. 465-469 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H.Shimizu,S.Tamura,Y.Ishihara,S.Shioya,K.Suga: "Control of molecular weight and molefradion in poly(-D(-)-3-hydroxyalkanoate(PHA) production by Alcaligenes eutrophus" Proc.of 3rd International Scientific Workshop on Biodegradable Plastics and Polymers,Osaka. (予定).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hiroshi SHIMIZU, Shinji SONOO, Suteaki SHIOYA and Ken-ichi SUGA: "Production of poly-3-hydroxy butyric acid (PHB) by Alcaligenes eutrophus H16 in a fedbatch culture" Proceeding of Asia-Pacific Biochemical Engineering Conference 1992, Yokohama. 195-197 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hiroshi SHIMIZU, Shinji TAMURA, Suteaki SHIOYA and Ken-ichi SUGA: "Kinetic study of poly-D(-)-3-hydroxybutyric acid (PHB) production and its molecular weight distribution control in a fed-batch culture of Alcaligenes eutrophus" Journal of Fermentation and Bioengineering. 76. 465-469 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hiroshi SHIMIZU, Shinji TAMURA, Yoshiyuki ISHIHARA, Suteaki SHIOYA and Ken-ichi SUGA: "Control of Molecular Weight Distribution and Mole Fraction in Poly(-D(-)-3-hydroxy-alkanoate)(PHA) Production by Alcaligenes eutrophus" Proceeding of 3rd International Scientific Workshop on Biodegradable Plastics and Polymers, Osaka.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H.Shimizu,S.Sonoo,S.Shioya,K.Suga: "Production of poly-3-hydroxy butyric acid (PHB) by Alcaligenes eutrophus H16 in a fed-batch culture" Proc.of Asia-Pacific Biochemical Engineering Conference 1992,Yokohama. 195-197 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] H.Shimizu,S.Tamura,Y.Ishihara,S.Shioya,K.Suga: "Control of molecular weight and mole fraction in poly (-P(-)-3-hydroxy alkanoate)(PHA) production by Alcaligenes eutrophus" Proceeding of 3rd International Scientific Workshop on Biodeg radable Plastics and Polymers,osaka. 予定. (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] H.Shimizu,S.Tamura,S.Shioya,K.Suga: "Kinetic study of poly-D(-)-3-hydroxy butyric acid(PHB) production and its molecular weight distribution control" Journal of Fermentation and Bioengineering. 76. 465-469 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] H.Shimizu,S.Sonoo,S.Shioya,K.Suga: "Production of poly-3-hydroxy butyric acid(PHB) by Alcaligenes eutrophus H16 in a fed-batch culture." Proc.of Asia-Pacific Biochemical Engineering Conference 1992,Yokohama. 195-197 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] H.Shimizu,S.Sonoo,S.Shioya,K.Suga: "Production of Polyー3ーhydroxybutyric Acid(PHB) by Alcaligenes leutrophus H16 in a Fedーbatch Culture" Proceeding of AsiaーPacific Biochemical Engineering Conference 1992,Yokohama. (1992)

    • Related Report
      1991 Annual Research Report

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Published: 1992-04-01   Modified: 2021-08-23  

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