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GENES STRUCTURES, EXPRESSIONS, REGULATIONS AND MECHANISMS OF EXCREATION OF PECTINOLYTIC ENZYMES

Research Project

Project/Area Number 03453135
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 応用微生物学・発酵学
Research InstitutionTOHOKU UNIVERSITY

Principal Investigator

IZAKI Kazuo  FACULTY OF AGRICULTURE, TOHOKU UNIVERSITY, PROFESSOR, 農学部, 教授 (80005587)

Co-Investigator(Kenkyū-buntansha) KAMIO Yoshiyuki  FACULTY OF AGRICULTURE, TOHOKU UNIVERSITY, ASSOCIATE PROFESSOR, 農学部, 助教授 (00109175)
Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥6,600,000 (Direct Cost: ¥6,600,000)
Fiscal Year 1992: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1991: ¥5,800,000 (Direct Cost: ¥5,800,000)
Keywordspectate lyase gene / pectate lyase / pectin lyase / pectin lyase gene / palindrome / excretion of pectate lyase / ペクチンリアーゼ遺伝子のクローン化 / ペクチンリアーゼ遺伝子構造 / SOS機能 / ペクチン酸リア-ゼ / ペクチン酸リア-ゼ遺伝子 / ペクチン酸リア-ゼ分泌 / 軟腐病菌 / ペクチン酸リア-ゼ遺伝子のクロ-ン化
Research Abstract

Genes structures of pectate lyase and pectin lyase of Erwinia carotovora Er, their expression and regulation and excretion of pectate lyases from the cells were studied in this research. Firstly, pectate lyase gene II was cloned and the complete nucleotides of pectate lyase gene II were sequenced in addition to pectate lyase genes I and III which were already determined. Complete nucleotides of pectate lyase I were also sequenced and some sequences were revised. Pectate lyase genes I.II.III were found to be closely and tandemly lined as a cluster. Secondly, the complete nucleotides of pectin lyase gene were sequenced, and found to be composed of 942 bp. From this result, pectin lyase was considered to be a protein of molecular mass of 33,700 composed of 314 amino acid residues. Regions of -35, -30, SD sequence and two parindromic regions were found in the 5'upstream of pectin lyase. However, Lex A binding site which is found in operator region of SOS regulon of E.coli. From the experiment using the deletion mutants in the 5'upstream region of pectin lyase, expression of pectin lyase genes seemed to be not controlled by repressor as in SOS reaction. Thirdly, pectate lyases I,II,III were not excreated from the cells of E.coli, while they were excreated efficiently from the cells of Erwinia carotovora Er. Further experiments are necessary to explain this difference.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Aruto Yoshida,Kazutoshi Ito,Yoshiyuki Kamio,Kazuo Izaki: "Parification and Properties of Pectate lyase III of Erwinia carotovora Er" Agric.Biol.Chem.55. 601-602 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Aruto Yoshida,Miho Izuta,Kazutoshi Ito,Yoshiyuki Kamio,K.Izaki: "Cloning and Characterization of the Pectate Lyase III hene of Erwinia carotovora Er" Agric.Biol.Chem.55. 933-940 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Hiroki Ohnishi,Tamotsu Nishida,Aruto Yoshida,Yoshiyuki Kamio,Kazuo Izaki: "Nucleotide sequence of pnl gene from Erwinia carotovora Er" Biochem.Biophys.Res.Commun.176. 321-327 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Aruto Yoshida,Yushi Matsuo,Yoshiyuki Kamio,Kazuo Izaki: "Molecular Cloning and sequence of the extracellalar pectate lyase II gene from Erwinia carotovora Er" Biosci.Biotech.Biochem.56. 1596-1600 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 二階堂 直樹,大西 博喜,神尾 好是,伊崎 和夫: "細菌のペクチンリアーゼ遺伝子,DNA合成阻害剤により誘導される新規の発現調節機構が存在か?" 化学と生物. 30. 345-347 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 伊崎 和夫: "野菜軟腐病菌のペクチン質分解酵素ータンパク質とその遺伝子ー" バイオサイエンスとインダストリー. 50. 11-17 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Aruto Yoshida, Kazutoshi Ito, Yoshiyuki Kamio and Kazuo Izaki: "Purification and Properties of Pectate Lyase III of Erwinia carotovora Er." Agric. Biol. Chem.55. 601-602 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Aruto Yoshida, Miho Izauta, Kazutoshi Yoshida, Yoshiyuki Kamio and Kazuo Izaki: "Cloning and Characterization of the Pectate Lyase III gene of Erwinia carotovora Er." Agric. Biol. Chem.55. 933-940 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Hiroki Ohnishi, Tamotsu Nishida, Aruto Yoshida, Yoshiyuki Kamio and Kazuo Izaki: "Nucleotide Sequence of PNL Gene from Erwinia carotovora Er." Biochem. Biophys. Res. Commun.176. 321-327 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Aruto Yoshida, Yushi Matsuo, Yoshiyuki Kamio and Kazuo Izaki: "Molecular Cloning and Sequence of the Extracellular Pectate Lyase II Gene from Erwinia carotovora Er." Biosci. Biotech. Biochem.56. 1596-1600 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Naoki Nikaidou, Hiroki Ohnishi, Yoshiyuki Kamio and Kazuo Izaki: "Pectin Lyase Gene of Soft-Rot Bacteria; Presence of New Regulation of Gene Expression Induced by Inhibitor of DNA Syntheis ? Kagaku to Seibutsu" 30. 345-347 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kazuo Izaki: "Pectinolytic Enzymes of Soft-Rot Bacteria-Proteins and Their Genes. Bioscience and Industry" 50. 11-17 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] A.Yoshida,Y.Matsuo Y.Kamio and K.Izaki: "Molecularcloning and sequencing of the extracellular pectate lyase II gene from Erwinia carotovora Er" Biosci. Biotech. Biochem.56. 1596-1600 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 伊崎 和夫: "野菜軟腐病菌のペクチン質分解酵素 ータンパク質とその遺伝子ー" バイオサイエンスとインダストリー. 50. 315-321 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 二階堂 直樹,大西 廣喜 神尾 好是,伊崎 和夫: "細菌のペクチンリアーゼ遺伝子 DNA合成阻害剤により誘導される新規の発現調節機構が存在か?" 化学と生物. 30. 345-347 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] A.Yoshida,K.Ito,Y.Kamio and K.Izaki: "Purification and Properties of Pectate Lyase III of Erwinia carotovora Er" Agric.Biol.Chem.,. 55. 601-602 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] A.Yoshida,M.Izuta,K.Ito,Y.Kamio and K.Izaki: "Cloning and Characterization of the Pectate lyase III gene of Erwinia carotovora Er" Agric.Biol.Chem.,. 55. 930-940 (1991)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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