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Biochemical studies on dwarf-8 (D_8) seedlings of Zea mays L.

Research Project

Project/Area Number 03454006
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 植物生理学
Research InstitutionUniversity of Tsukuba

Principal Investigator

SAKAI Shingo  University of Tsukuba, Institute of Biological Sciences, Associate Professor, 生物科学系, 助教授 (60033388)

Project Period (FY) 1991 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1993: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1992: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1991: ¥2,600,000 (Direct Cost: ¥2,600,000)
KeywordsZea mays L. / dwarf-8 / gibberellin / gibberellin-binding proteins / 矮性株(D_8)
Research Abstract

Gibberellin-binding proteins (GBPs) were extracted from dark-grown maize seedlings of dwarf-8 (D_8) and its normal allele. The proteins were partially purified by affinity chormatography on GA_3-linked EAH-Sepharose 4B.On the basis of Scatchard plot analysis, GBPs from normal seedlings contained a high affinity component of Kd for GA_4 of o.3 uM.While the high affinity component was not detected in GBPs from D_8 seedlings but they contained a low affinity component of Kd of 13uM.The binding of H-GA_4 to GBPs from normal seedlings was inhibited by GA_1 and GA_3, but that from D_8 seedlings was weakly inhibited by GA_1 and GA_3. GA_<13>, an inactive GA, had no effect on the binding. When GBPs were separated by native-PAGE, it was clarified that the GBPs from D_8 seedlings lacked one component (GBP-3) which existed in the GBPs from normal seedlings. The GBP-3 was purified from normal seedlings. The molecular weight was estimated to be about 280,000 by gel filtration on Sephacryl S-300. It consisted of one subunit with molecular weight of 70,000. The dissociation constant for GA_4 was 0.5 uM.From these results, we considered that GBP-3 might be a candidate of a gibberellin receptor lacked in D_8 seedlings of Zea mays. To clarify the working hypothesis, we are studying cDNA clining of GBP-3 using an antibody made against GBP-3.

Report

(4 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • 1991 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] M.Nakajima: "Partial purification of a soluble gibberellin-binding protein from mung bean hypocotyls." Plant Cell Physiol.34. 289-296 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] S.Sakai: "Isolation of gibberellin-binding proteins by affinity chromatography." Biosci.Biotech.Biochem.58(in press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] S.Sakai: "Properties of gibberellin-binding proteins from normal and dwarf-8(a dominant gibberellin non-responding dwarf mutant)seedlings of Zea mays L." Biosci.Biotech.Biochem.58(in press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M.Nakajima: "Partial purification of a soluble gibberellin-binding protein from mung bean hypocotyls." Plant Cell Physiol.34. 289-296 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] S.Sakai: "Isolation of gibberellin-binding proteins by affinity chromatography." Biosci.Biotech.Biochem.58, (in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] S.Sakai: "Properties of gibberellin-binding proteins from normal and dwarf-8 (a dominant gibberllin non-responding dwarf mutant) seedlings of Zea mays L." Biosci.Biotech.Biochem.58, (in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M.Nakajima: "Partial purification of a soluble gibberellin-binding protein from mung bean hypocotyls." Plant Cell Physiol.34. 289-296 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] S.Sakai: "Isolation of gibberellin-binding proteins by affinity chromatography." Biosci.Biotech.Biochem.58(in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] S.Sakai: "Properties of gibberellin-binding proteins from normal and dwarf-8(a dominant qibberellin non-responding dwarf mutant)seedlings of Zea mays L." Biosci.Biotech.Biochem.58(in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] S.Sakai: "Reguratory functions of soluble suxin-binding proteins." International Review of Cytology. 135. 239-267 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] S.Sakai and N.Kamei: "Purification of a soluble cytokinin-binding protein from etiolated mung bean seedlings." Biosci.Biotech.Biochem.56. 504-507 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] S.Sakai,M.Kikuchi and N.Nakajima: "Interaction between ABP-1 and RNA polymerase II." Biosci.Biotech.Biochem.56. 1225-1229 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] A.Oomiya,M.Kikuchi S.Sakai and T.Hayashi: "Purification and properties of an auxin-binding protein from shoot apex of peach tree." Plant Cell Physiol.

    • Related Report
      1992 Annual Research Report
  • [Publications] M.Nakajima,S.Sakai,K.Kanazawa,I.Yamaguchi,N.Takahashi,N.Murofushi: "Partial purification of a soluble gibberellin-binding protein from mung bean hypocotyls." Plant Cell Physiol.

    • Related Report
      1992 Annual Research Report
  • [Publications] Sakai Shingo: "Regulatory functions of soluble auxinーbinding proteins." International Review of Cytology. 135. 239-267 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Sakai Shingo: "Purification of a soluble cytokininーbinding protein from etiolated mung bean seedlings." Biosci.Biotech.Biochem.56. 504-507 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Sakai Shingo: "Interaction between auxinーbinding proteinーI and RNA polymerase II"

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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