Isolation and analysis of clock mutants in Neurospora crassa
| Project/Area Number |
03454010
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
植物生理学
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| Research Institution | OKAYAMA UNIVERSITY |
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Principal Investigator |
NAKASHIMA Hideaki Okayama Univ., Faculty of Sci., Professor, 理学部, 教授 (70142007)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAHORI Kiyoshi Okayama Univ., Faculty of Sci., Assistant, 理学部, 助手 (90231472)
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| Project Period (FY) |
1991 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥5,600,000 (Direct Cost: ¥5,600,000)
Fiscal Year 1992: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1991: ¥4,100,000 (Direct Cost: ¥4,100,000)
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| Keywords | CIRCADIAN / NEUROSPORA / CALCIUM / BIOLOGICAL CLOCK / サーカディアン / サ-カディアンリズム / カルシウム |
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| Research Abstract |
Several different strategies have been used for isolating new clock mutants in Neurospora crassa. First one was isolation of calcium metabolism mutants which showed different dependency on external concentration of calcium ions to their mycelial growth. Finally, we succeeded to isolate 6 mutants. One mutant showed different period length of conidiation rhythm and other one showed low sensitivity to light which results phase shifting. These data suggest that clock mechanism of Neurospora must be regulated or affected by calcium metabolism. Second strategy was isolation of temperature dependent clock mutants. We isolated several hundreds of temperature sensitive mutants and some mutants showed almost completely different phenotypic expression in circadian conidiation rhythm but at now we are examining these quite interesting temperature sensitive clock mutants.
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Report
(3 results)
Research Products
(18 results)
