| Project/Area Number |
03454048
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
園芸・造園学
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| Research Institution | KOBE UNIVERSITY |
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Principal Investigator |
HASHIMOTO Tohru Kobe Univ., Fac. Sci., Professor, 理学部, 教授 (60087616)
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| Co-Investigator(Kenkyū-buntansha) |
SHICHIJO Chizuko Kobe Univ., Fac. Sci., Research Technician, 理学部, 教務職員 (70226132)
TSURUMI Seiji Kobe Univ., Grad. Sch. Sci. & Tech., Lecturer, 自然科学研究科, 助手 (80144608)
SERA Akira Kobe Univ., Fac. Sci., Professor, 理学部, 教授 (90025271)
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| Project Period (FY) |
1991 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1992: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1991: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | Dwarf pea / GC / Growth inhibitors / HPLC / Pisum sativum / A-1-3 / Isomers / 大量抽出処理 / ガスクロマトグラフ |
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| Research Abstract |
In order to characterize novel growth inhibitors A-2 alpha and beta responsible for light growth inhibition in the dwarf pea cv. Progress No. 9 and to find an application to horticulture, their isolation was attempted from Progress pea shoots grown in the field. Although an pure isolation of A-2's was not successful because of their extreme instability, many pieces of information useful for a large-scale extraction and purification as well as for culture and storage of pea shoots were obtained as follows: irrespective of the culture seasons, culture periods, and hence of the different shapes of shoots, the main shoot as well as the shoots derived from lateral buds serve as extraction materials; freezing shoots immediately after harvesting is necessary, and storage at -20ﾟ C keeps the inhibitor content unchanged for years. Also, we succeeded in locating A-2 alpha,beta and alpha and A-3's as well as A-1-3's on reverse phase HPLC chromatograms, which serve greatly for detection and quantification of the inhibitors in isolation processes. We found novel inhibitors named A-1-3 alpha and beta, and succeeded in identifying their HPLC peaks. A-1-3 alpha and beta were found to be isomers which change from one another in solution.
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