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Analysis of nucleotide sequence of insecticidal crystal protein (ICP) gene which shows highly activity against Lepidoptera insect.

Research Project

Project/Area Number 03454058
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 蚕糸学
Research InstitutionHokkaido University

Principal Investigator

IIZUKA Toshihiko  Hokkaido Univ., Fac. of Agriculture PROFESSOR, 農学部, 教授 (50001441)

Co-Investigator(Kenkyū-buntansha) KIKUTA Harunori  Hokkaido College of Arts and Sciences ASSOCIATE PROFESSOR, 助教授
ASANO Shinichiro  Hokkaido Univ., Fac. of Agriculture ASSISTANT PROFESSOR, 農学部, 助手 (60222585)
BANDO Hisanori  Hokkaido Univ., Fac. of Agriculture ASSOCIATE PROFESSOR, 農学部, 助教授 (20189731)
Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥6,200,000 (Direct Cost: ¥6,200,000)
Fiscal Year 1992: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1991: ¥4,800,000 (Direct Cost: ¥4,800,000)
KeywordsBacillus thuringiensis / Insecticidal crystal protein / PCR method / Screening / Crystal protein / ICP gene / CryI gene / CryII gene / バチルス・チュ-リンゲンシス / スクリ-ニング / ICP遣伝子 / CryI遣伝子 / CryII遣伝子
Research Abstract

A insecticidal crystal protein (ICP) produced by Bacillus thuringiensis (BT), which shows highly activity against the Lepidopteran insect, should be studied from the following two ways:
1. Newly isolation and identification of BT. : Newly BT isolates from soil samples which were collected in the natural conserved area, were identified by routine technique of H-serotype and by newly developed technique depending on agarose gel electrophoresis of cryl genes. After oligo-primers designed by a specific domain were synthesized, these cryI genes were amplified by PCR method with template DNA from BT subspecies. The newly identification technique was very effective in order to clear a high active strain comparing with already known BT strains.
2. Nucleotide sequence analysis of ICP genes. : BT subsp. wuhanensis has been known as high active strain against the silkworm and the cabbage moth. According to a report of Iizuka et al. (1993), subsp. wuhanensis has included cryIA(b), cryIA(c) and cryID genes. In this study, the nucleotide sequence of cryIA(b) was analyzed and compared with another cryIA(b) genes from subspp. thuringiensis berliner 1715 and kurstaki HD-2. The differentiation of amino acid composition between wuhanensis and HD-2 was only one within a active domain. A regulatory mechanisms of each protein expression encoded by three genes was in progressed.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] 浅野 眞一郎 他: "DNAハイブリダイゼーション法によるBacillis thuringiensisの家蚕殺虫性活性伴定." 日本蚕糸学雑誌. 60. 475-479 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 浅野 眞一郎 他: "Cry2遺伝子プライマー合成によるBacillus thuringiensis分離株のCry2遺伝子同定." 日本蚕糸学雑誌. (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 浅野 眞一郎 他: "PCR法によるBacillus thuringiensis Cry2遺伝子同定." 日本蚕糸学雑誌. (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Asano,S. and T.Iizuka: "Identification of insecticidal activity of Bacillus thuringiensis against the silkworm by DNA hybridization method." J. Seric. Sci. Jpn.60. 475-479 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Asano,S., H.Bando, H.Kikuta and T.Iizuka: "Identification of cryII genes in Bacillus thuringiensis isolates by oligo-nucleotide DNA primers." J. Seric. Sci. Jpn.(1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Asano,S., H.Bando, and T.Iizuka: "The amplification and identification of cryII genes from Bacillus thuringiensis by PCR procedure." J. Seric. Sci. Jpn.(1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 浅野 眞一郎 他: "DNAハイブリダイゼーション法によるBacillus thuringiensisの家蚕殺虫性活性判定." 日本蚕糸学雑誌. 60. 475-479 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] 浅野 眞一郎 他: "CryII遺伝子プライマー合成によるBacillus thuringiensis分離株のCryII遺伝子同定." 日本蚕糸学雑誌. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 浅野 眞一郎 他: "PCR法によるBacillus thuringiensis CryII遺伝子同定." 日本蚕糸学雑誌. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] IIZUKA,T.et al.: "Analysis of nucleotide sequence in ICP gene from Bacillus thuringiensis subsp.wuhanensis." Proc.of the XXV Annual Meeting of Society for Invertebrate Pathology at Heidelberg. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] BANDO,H.et al.: "Structural analysis on the singleーstranded genomic DNAs of the virus newly isolated from silkworm: the DNA molecules share a common terminal sequence." Arch.Virology.KM/83. (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 浅野 眞一郎 他: "DNAハイブリダイゼ-ション法によるBacillus thuringiensisの家蚕殺虫活性判定." 日本蚕糸学雑誌. 60. 475-479 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] KIKUTA,H.et al.: "Preparation of ICP genes type specific oligonucleotide primer and application to classification of native Bacillus thuringiensis isolates." Proc.of XXV Annual Meeting of Society for Invertebrate Pathology at Heidelberg. (1992)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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