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Studies on the Suppression of Allergenic Proteins in Rice Seeds by Genetic Engineering

Research Project

Project/Area Number 03454068
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 製造化学・食品
Research InstitutionNagoya University

Principal Investigator

NAKAMURA Ryo  Nagoya University, School of Agriculture, Professor, 農学部, 教授 (70023398)

Co-Investigator(Kenkyū-buntansha) ADACHI Takahiro  Nagoya Univ., School of Agriculture, Assistant Professor, 農学部, 助手 (50222625)
MATSUDA Tsukasa  Nagoya Univ., School of Agriculture, Associate Professor, 農学部, 助教授 (20144131)
Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥5,200,000)
Fiscal Year 1992: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1991: ¥4,200,000 (Direct Cost: ¥4,200,000)
KeywordsRice Allergenic Protein / Anti-Sense RNA / Allergenic Protein Promoter / Transgenic Plant / Rice Seeds / Genetic Engineering Study / シスエレメント / 転写調節領域 / ゲノムDNA
Research Abstract

A genomic clone containing two genes of rice allergenic proteins (RA) was isolated from a rice genomic DNA library. For each RA gene putative TATA and CAAT boxes existed about 50 and 150bp upstream of the transcription initiation site, respectively. Southern blotting analysis also showed that that the allergenic proteins were encoded by a multigene family containing at least four members. To suppress expression of the RA gene during rice seed ripening by anti-sense RNA, anti-sense RA genes were constructed with some plant gene promoters including the RA promoter, and introduced into rice protoplast by electroporation. Transformed cells were selected and grown to maturity. Rice seeds harvested from some of such transgenic plants contained reduced amount of RA, suggesting that the anti-sense RNA method was effective to suppress the RA gene expression in ripening rice seeds.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] T.Matsuda,el al.: "Immunochemical Studies on Rice Allergenic Proteins." Agric.Biol.Chem. 55. 509-513 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] A.Urisu,et al.: "Rice Protein 16kD-A Major Allergen in Rice Grain Extract." Jpn.J.Allergol.40. 1370-1376 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] A.Urisu,et al.: "16-Kilodalton Rice Protein Is One of the Major Allergens in Rice Grain Extract and Responsible for Cross-Allergenicity between Cereal Grains in the Poaceae Family." Int.Arch.Allergy Appl.Immunol.96. 244-252 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] H.Izumi,el al.: "Nucleotide Sequece of cDNA Clone Encoding A Major Allergenic Protein in Rice Seeds.Homology of the Deduced Amino Acid Sequece with Members of α-Amylase/Trypsin Inhibitor Family." FEBS Lett.302. 213-216 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.Adachi,et al.: "Gene structure and expression of rice seed allergenic proteins belonging to the α-amylase/trypsin inhibitor family." Plant Mol.Biol.21. 239-248 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 中村 良: "穀物のアレルゲン物質とその軽減化" 農業および園芸. 67. 205-209 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T. Matsuda, et al.: "Immunochemical Studies on Rice Allergenic Proteins." Agric. Biol. Chem.55. 509-513 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] A. Urisu, et al.: "Rice Protein 16KD - A Major Allergen in Rice Grain Extract." Jpn. J. Allergol.40. 1370-1376 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] A. Urisu, et al.: "16-Kilodalton Rice Protein Is One of the Major Allergens in Rice Grain Extract and Responsible for Cross-Allergenicity between Cereal Grains in the Poaceae Family." Int. Arch. Allergy Appl. Immunol.96. 244-252 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] H. Izumi, et al.: "Nucleotide Sequence of a cDNA Clone Encoding A Major Allergenic Protein in Rice Seeds, Homology of the Deduced Amino Acid Sequence with Members of alpha-Amylase/Trypsin Inhibitor Family." FEBS Lett.302. 213-216 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T. Adachi, et al.: "Gene Structure and Expression of Rice Seed Allergenic Proteins Belonging to the alpha-Amylase/Trypsin Inhibitor Family." Plant Mol. Biol.21. 239-248 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.Adachi,et al.: "Gene structure and expression of rice seed allergenic proteins belonging to the α-alylase/trypsin inhibitor family." Plant Mol.Biol. 21. 239-248 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 松田 幹: "Immunochemical Studies on Rice Allergenic Proteins." Agric.Biol.Chem.55. 509-513 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 中村 良: "穀物のアレルゲン物質とその軽減化" 農業および園芸. 67. 205-209 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 和泉 秀彦: "Nucleotide sequence of a cDNA clone encoding a major a major allergenic protein in rice seedsーHomology of the deduced amino acid sequence with members of αーamylase/trypsin inhioitor family."

    • Related Report
      1991 Annual Research Report
  • [Publications] 中村 良: "食品分析学(アレルゲン成分の解析)" 文永堂出版, 203-218 (1991)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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