| Project/Area Number |
03454087
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Fisheries chemistry
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| Research Institution | University of Tokyo |
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Principal Investigator |
YAMAGUCHI Kataumi University of Tokyo Faculty of Agriculture Professor, 農学部, 教授 (50011896)
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| Co-Investigator(Kenkyū-buntansha) |
MURAKAMI Masahiro University of Tokyo Faculty of Agriculture Associate Professor, 農学部, 助教授 (70134517)
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| Project Period (FY) |
1991 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1992: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1991: ¥4,600,000 (Direct Cost: ¥4,600,000)
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| Keywords | Microalgae / Microcystis aeruginosa / Enzyme inhibitor / Protease inhibitor / Trypsin inhibitor / Peptide / Erastase inhibitor / Micropeptin / 藍藻 / 酵素阻害 / トリプシン / エラスタ-ゼ / パパイン / キモトリプシン |
|---|
| Research Abstract |
In the screening of serine protease inhibitors in microalgae, the blue green alga Microcystis aeruginosa (NIES-100), which produced the angiotensin converting enzyme inhibitive peptide, micropeptin showed potent inhibition against trypsin. Micropeptins A(1) and B(2) isolated from the cultured alga. The gross structures of micropeptins A and B were elucidated on the basis of the chemical degradation and extensive NMR analyses including ^1H-^1H COSY, HMQC, HOHAHA, HMQC-HOHAHA and ROESY. Micropeptins contained Ahp like related cyclic depsipeptide dolastatin 13 isolated from Dolabella auricularia. They inhibited trypsin and plasmin at very low concentration.
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