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GONADOTROPIN RELEASING MECHANISM VIA RECEPTORS OF PITUITARY CELLS IN POULTRY

Research Project

Project/Area Number 03454097
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 畜産学(含草地学)
Research InstitutionGIFU UNIVERSITY

Principal Investigator

TANAKA Katuhide  GIFU UNIVERSITY, FACULTY OF AGRICULTURE, Professor, 農学部, 教授 (20021678)

Co-Investigator(Kenkyū-buntansha) KAWASHIMA Mitsuo  GIFU UNIVERSITY, FACULTY OF AGRICULTURE, ASSOCIATE Professor, 農学部, 助教授 (10177686)
KAMIYOSHI Michiharu  GIFU UNIVERSITY, FACULTY OF AGRICULTURE, Professor, 農学部, 教授 (00021709)
Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 1992: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1991: ¥4,300,000 (Direct Cost: ¥4,300,000)
KeywordsGonadotropin-releasing hormone receptor / Luteinizing hormone / Pituitary cell culture / Progesterone receptor / Estradiol-17beta / Protein synthesis inhibitor / 5alpha-dihydrotestosterone / Chicken / 下垂体前葉 / 性腺刺激ホルモン / 性腺刺激ホルモン放出ホルモン / プロジェステロン / エストラジオール / ホルモン受容体 / エストラジオ-ル / 蛋白合成阻害剤
Research Abstract

(1) Gonadotropin-releasing hormone receptors having a high affinity and low capacity have been found in the hen pituitary cells. The equilibrium dissociation constant of the receptor and the maximum number of binding sites per milligram of membrane protein were greater in laying hens than in nonlaying hens. A single i.m. injection of progesterone, estradiol-17beta, or 5alpha-dihydrotestosterone into nonlaying hens caused an increase in Kd and Bmax values. During an ovulatory cycle of the laying hen, Kd and Bmax values decreased from 16 to 14 h and 8 to 6 h before ovulation. A single i.v. injection of chicken LHRH-1 caused a decrease in the Kd and Bmax values within 2 min after the injection. It is suggested that the hen pituitary gonadotropin-releasing hormone receptor bindings are affected by ovarian steroid hormones and that their changes during the ovulatory cycle may relate to the release of a gonadotropin from the pituitary. (2) The amount of progesterone receptor in the cytosolic fraction of the pituitary cells of the hen was found to increase when incubated with estradiol-17beta for 1-4 h, but not when incubated with the presence of cycloheximide, actinomycin-D, or alpha-amanitin. It is suggested that the cytosolic progesterone receptor binding of the hen pituitary cells was enhanced by the action of estrogen through a protein synthesizing pathway. (3) The cellular content of Luteinizing hormone was found to increase when the pituitary cells were preincubated with estradiol-17beta for 2 h and then incubated with progesterone for 4 h. The increase in the cellular LH in response to progesterone was dose dependent. Protein synthesis inhibitors (cycloheximide, actinomycin-D, and alpha-amanitin) blocked the response of the cells to progesterone. It is suggested that LH production in estrogen-primed pituitary cells of the hen was stimulated by progesterone through a protein-synthesizing pathway.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Kawashima,M.,Ukai,A.,Kamiyoshi,M.,and Tanaka,K.: "Enhancement of progesterone receptor bindings by estradiol in pituitary cells of the hen" POULTRY SCIENCE. 71. 352-356 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kawashima,M.,Takeo,H.,Kamiyoshi,M.and Tanaka,K.: "Luteinizing hormone-releasing hormone receptor bindings of the hen pituitary" POULTRY SCIENCE. 71. 1079-1086 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kawashima,M.,Ukai,A.,Kamiyoshi,M.,and Tanaka,K.: "Effect of progesterone in vitro on luteinizing hormone production in hen pituitary cells pretreated with estrogen" POULTRY SCIENCE. 71. 1215-1220 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kawashima,M.,Kamiyoshi,M.and Tanaka,K.: "Estrogen receptor binding in the hen hypothalamus and pituitary during the ovulatory cycle" POULTRY SCIENCE.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kawashima,M.,Kamiyoshi,M.,and Tanaka,K.: "Estrogen reduces sensitivity of chicken pituitary cells to" JAPANESE POULTRY SCIENCE.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kawashima,M., Ukai,A., Kamiyoshi,M., and Tanaka,K.: "Enhancement of progesterone receptor bindings by estradiol in pituitary cells of the hen" Poultry Science. 71. 352-356 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kawashima,M., Takeo,H., Kamiyoshi,M., and Tanaka,K.: "Luteinizing hormone-releasing hormone receptor bindings of the hen pituitary" Poultry Science. 71. 1079-1086 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kawashima,M., Ukai,A., Kamiyoshi,M., and Tanaka,K.: "Effect of progesterone in vitro on Luteinizing hormone production in hen pituitary cells pretreated with estrogen" Poultry Science. 71. 1215-1220 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kawashima,M., M,Kamiyoshi, and Tanaka,K.: "Estrogen receptor binding in the hen hypothalamus and pituitary during the ovulatory cycle" Poultry Science.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kawashima,M., Kamiyoshi,M., and Tanaka,K.: "Estrogen reduces sensitivity of chicken pituitary cells to GnRH" Japanese Poultry Science.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Mitsuo Kawashima,A.Ukai,M.Kamiyoshi,K.Tanaka: "Enhancement of progesterone receptor bindings by estradiol in pituitary cells of the hen" Poultry Science. 71. 352-356 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] M.Kawashima,H.Takeo,M.Kamiyoshi,K.Tanaka: "Lueinizing hormone-releasing hormone receptor bindings of the hen pituitary" Poultry Science. 71. 1079-1086 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] M.Kawashima,A.Ukai,M.Kamiyoshi,K.Tanaka: "Effect of progesterone in vitro on luteinizing hormone production in hen pituitary cells pretreated with estrogen" Poultry Science. 71. 1215-1220 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] M.Kawashima,M.Kamiyoshi,K.Tanaka: "Estrogen receptor binding in the hen hypothalamus and pituitary during the ovulatory cycle" Poultry Science.

    • Related Report
      1992 Annual Research Report
  • [Publications] M.Kawashima,M.Kamiyoshi,K.Tanaka: "Estrogen reduces sensitivity of chicken pituitary cells to GnRH" Japanese Poultry Science.

    • Related Report
      1992 Annual Research Report
  • [Publications] Mitsuo Kawashima,A.Ukai,Michiharu Kamiyoshi,and Katuhide Tanaka: "Enhancement of progesterone receptor bindings by estradiol in pituitary cells of the hen" Poultry Science. 71. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Mitsuo Kawashima,H.Takeo,Michiharu Kamiyoshi,and Katuhide Tanaka: "Luteinizing hormoneーreleasing hormone receptor bindings of the hen pituitary:Difference between laying and nonlaying hens,effects of ovarian steroid hormones in vivo,and changes during an ovulatory cycle" Poultry Scinece. 71. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Hitsuo Kawashima,A.Ukai,Michiharu Kamiyoshi,and Katuhide Tanaka: "Effect of progesterone in vitro on luteinizing hormone production in hen pituitary cells pretreated with estrogen" Poultry Science. 71. (1992)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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