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Pathogenesis of encephalomyocarditis virus-induced bi-phasic paralysis

Research Project

Project/Area Number 03454105
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 基礎獣医学
Research InstitutionThe University of Tokyo

Principal Investigator

DOI Kumio  Univ. of Tokyo, Agriculture, Professor, 農学部, 教授 (70155612)

Co-Investigator(Kenkyū-buntansha) TSUBONE Hirokazu  Univ. of Tokyo, Agriculture, Assoc. Professor, 農学部, 助教授 (30142095)
SHIOTA Kumio  Univ. of Tokyo, Agriculture, Assoc. Professor, 農学部, 助教授 (80196352)
TAKAHASHI Eiji  Univ. of Tokyo, Agriculture, Professor, 農学部, 教授 (50183439)
GOTO Naoaki  Univ. of Tokyo, Agriculture, Professor, 農学部, 教授 (70011989)
Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥5,500,000 (Direct Cost: ¥5,500,000)
Fiscal Year 1992: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1991: ¥3,500,000 (Direct Cost: ¥3,500,000)
Keywordsbi-phasic paralysis / encephalomyocarditis virus / mouse / helper / inducer T cell / macrophage / water-maze task performance / DNA probe / 脳心筋ウイルス / 後肢麻痺 / 中枢神経病変
Research Abstract

(1) Establishment of a model of bi-phasic paralysis We established a model of bi-phasic paralysis by inoculating 10^1PUF/head of EMC-D into 8-week-old male DBA/2 mice.
(2) Sequence and pathogenesis of bi-phasic paralysis Transient hind limb paralysis developed by 10 days post inoculation (DPI) and it disappeared by 28 DPI. Thereafter recurrence of paralysis occured in about 30% of the recovered mice by 56 DPI. Virological, pathological and immunohistopathological examinations of the spinal cord revealed that direct viralattack to neurons and oligodendrocytes might play an important role in the development of initial paralysis and helper/inducer T cells and macrophages in the development of recurrent paralysis.
(3) Water-maze task performance The influence of EMC-D on water-maze task performance was examined in DBA/2 and BALB/c mice. The disturbance of water-maze task performance observed in these mice might be partially due to the dysfunction of memory for working process or cognition of orientation other than underlying decreased ability in locomotion.
(4) DNA probes for virus RNA of EMC-D DNA probes for virus RNA of WMC-D were made and usefulness of these DNA probes was confirmed.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Takeda M.et al.: "Lesions in central nervous system of DBA/2 mice infected with The D variant of encephalomyocarditis virus(EMC-D)" Journal of Veterinary Medical Science. 53. 1013-1017 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Takeda M.et al.: "Bi-phasic desease of central nervous system induced in DBA/2 mice by The D variant of encephalomyocarditis virus(EMC-D)" International Journal of Experimental Pathology. 74. (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Yayou K.ea al.: "The disturbance of water-maze task performance in mice with EMC-D virus infection" Journal of Veterinary Medical Science. 55. (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Doi C.et al.: "Tronsmissibility of The D variant of eucephalomyocarditis vinus(EMC-D) in mice" Laboratory Animals. 29. (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Yamaguchi K.ea al.: "Protection of mice from a lethal coronavirus infection in the central nervous system by adoptive transfer of virus-specific T cell clones." Journal of Neuroimmunology. 32. 1-9 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Katayama T.et al.: "Paracrine effect of Folliculostellate cells on The growth factor-like action of activin A in anterior coll cuture." Endoclinology Japonica. 39. 289-297 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Takeda M., Hirasawa K. & Doi K: "Lesions in the central nervous system of DBA/2 mice infected with the D variant of encephalomyocarditis virus (EMC-D)." J.Vet.Med.Sci.53. 1013-1017 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Takeda M., Itagaki S., Doi K: "Bi-phasic disease of central nervous system induced in DBA/2 mice by the D variant of encephalomyocarditis virus (EMC-D)." Int.J.Exp.Pathol.74.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Yayou K., Takeda M., Tsubone H., Sugano S. & Doi K: "The disturbance of water-maze task performance in mice with EMC-D virus infection." J.Vet.Med.Sci.55.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Doi C., Takeda M., Hirasawa K. & Doi K: "Transmissibility of the D variant of encephalomyocarditis virus (EMC-D) in mice." Lab.Anim.27.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Yamaguchi K., Goto N., Kyuwa S., Hayami M. & Toyoda Y: "Protection of mice form a lethal coronavirus infection in the central nervous system by adoptive transfer of virus-specific T cell clones." J.Neuroimmunol.32. 1-9 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Katayama T., Shiota K., Sugino H. & Takahashi M: "Paracrine effect of Folliculo-stellate cells on the growth factor-like action of activin A in anterior cell cultures." Endocrinol. Japon.39. 289-297 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Takeda M,et al.: "Bi-phasic disease of central nervous system induced in DBA/2 mice by the Dvariant of encephalomyocavrlitis virus(EMC-D)" International Journal of Experimental Patholgy. 74. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Yayou K.et al.: "The disturbance of water-maze tosk porformonce in mice with EMC-D viruo infection" The Journal of Vcterinary Medical Scionce. 55. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Doi C.et al.: "Transmissibility of the Dvoriont of oncephalo-myocorditis virus(EMC-D)in mice" Laboratory Animals. 29. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Sugawara,Y.et al.: "Acute infection of encephalomyocarditis virus in Syrian hamsters." Journal of Veterinary Medical Science. 53. 463-468 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Takada,M.et al.: "Lesions in the central nervous system of DBA/2 mice infected with the Dvariant of encephalomyocanditis virus." Journal of Veterinary Medical Science. 53. 1013-1017 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Takeda,M.et al.: "Ultrastructural changes in the central nervous system of mice infected with enccphalomyocarditis virus" Journal of Veterinary Medical Science.

    • Related Report
      1991 Annual Research Report
  • [Publications] Taked,H.et al: "Characterization of mononuclear cells infiltrated in the central nervous lesions" Journal of Veterinary Medical Science.

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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