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Modulation of peristalsis by neurotensin and evidence for mechanism of generation, in the intestine

Research Project

Project/Area Number 03454107
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 基礎獣医学
Research InstitutionGifu University

Principal Investigator

OHASHI Hidenori  Gifu University, Faculty of Agriculture, Professor, 農学部, 教授 (40001531)

Co-Investigator(Kenkyū-buntansha) TAKEWAKI Tadashi  Gifu University, The United Graduate School of Veterinary Sciences (Doctoral Cou, 大学院・連合獣医学研究科, 教授 (00021717)
KOMORI Seiichi  Gifu University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (70195866)
Project Period (FY) 1991 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥4,900,000 (Direct Cost: ¥4,900,000)
Fiscal Year 1993: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1992: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1991: ¥3,900,000 (Direct Cost: ¥3,900,000)
KeywordsNeurotensin / Peptide / Smooth muscle / Voltage-dependent Calcium channel / GTP-protein / Signal transduction / Phospholipase C / Protein kinase C / 腸管平滑筋 / 興奮性神経伝達物質 / アパミン / 収縮作用 / 弛緩作用 / 膜電流 / カルシュウムイオン / 電位依在性カルシュウム電流 / 腸管の蠕動反射 / ニュ-ロテンシン / 脳腸ペプチド / 細胞膜電流 / カルシュムイオン
Research Abstract

Our studies were designed to determine whether endogenous neurotensin (tridecapeptide) (NT) participates in the regulation of peristalsis of the intestine.
In the rectum of the fowl, all of the major characteristics of NT suggest a possible role for NT as a mediator in the excitatory non-adrenergic and non-cholinergic (NANC) transmission : NT is present in Remak ganglia and the rectum in which cell bodies and axon terminals of NANC neurones are located, respectively, NT causes the muscle membrane to depolarize, and NT has an ability to abolish the responses of the rectal muscle to stimulation of NANC neurones as well as the peptide itself.
In guinea-pig intestine, NT, whether acts directly or indirectly, may be involved in regulation of the peristalsis in the intestine. Its predominant effect is inhibitory in the upper region of the intestine in which discharge rate of peristaltic waves is higher, but excitatory in the lower region in which discharge rate of peristaltic waves is lower.
NT owes the greater part of the contractile effect to an indirect action brought about by releasing ACh from cholinergic nerves in the longitudinal muscle of the small intestine. The contractile effects of NT on the longitudinal muscle of the large intestine, taenia caecum and circular muscle are attributable to a direct action. The relaxant effects of NT are all attributable to a direct action and blocked by apamin. Patch-clamp studies showed that NT produces an increase in activation of voltage-gated calcium cannel through a G-protein.
In beta-escin-skinned smooth muscle, NT potentiates carbachol- and caffeine-induced tension developments brought about by release of Ca2+ stores. The potentiation may result from an increase in Ca2+ sensitivity of contractile elements, in which activation of phospholipase C through a pertussis toxin-sensitive G-protein and activation of PKC by diacylglycerol seem to be operated as a signal transduction system.

Report

(4 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • 1991 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Kumiko Sugiyama: "Stimulative effect of sodium nitroprusside on peristaltic reflex in isolated guinea pig ileal segment" Japanese Journal of Pharmacology. 57. 279-289 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Seiichi Komori: "GTP-binding protein involvement in membrane currents evoked by carbachol and histamine in guinea-pig ileal muscle" Journal of Physiology. 450. 105-126 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Seiichi Komori: "Membrane potential and current responses to neurotensin in the longitudinal muscle of the rectum of the fowl" British Journal of Pharmacology. 107. 790-796 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hidenori Ohashi: "Neurotensin as a candidate for the excitatory transmitter of the non-adrenergic and non-cholinergic nerves in chicken rectum" Symposium on Autonomic Neuroeffector Mechanism:Abstract. 43 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hidenori Ohashi: "Mechanical and current responses to neurotensin in the smooth muscle of guinea-pig intestine" Journal of Autonomic Pharmacology. 14. 239-251 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Seiichi Komori: "Potentiation by neurotensin of carbachol-induced tension development in Ca2+-free solution in skinned guinea-pig ileal muscle" Japanese Journal of Pharmacology. 64. 214P (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kumiko Sugiyama et al.: "Stimulative effect of sodium nitroprusside on peristaltic reflex in isolated guinea pig ileal segment" Journal of Physiology. 57. 279-289 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Seiichi Komori et al.: "GTP-binding protein involvement in membrane currents evoked by carbachol and histamine in guinea-pig ileal muscle" Japanese Journal of Pharmacology. 450. 105-126 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Seiichi Komori et al.: "Membrane potential and current responses to neurotensin in the longitudinal muscle of the rectum of the fowl" British Journal of Pharmacology. 107. 790-796 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hidenori Ohashi et al.: "Neurotensin as a candidate for the excitatory transmitter of the non-adrenergic and non-cholinergic nerves in chicken rectum" Symposium on Autonomic Neuroeffector Mechanism : Abstract. 43. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hidenori Ohashi et al.: "Mechanical and current responses to neurotensin in the smooth muscle of guinea-pig intestine" Journal of Autonomic Pharmacology. 14. 239-251 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Seiichi Komori et al.: "Potentiation by neurotensin of carbachol-induced tension development in Ca2+-free solution in skinned guinea-pig ileal muscle" Japanese Journal of Pharmacology. 64, Suppl.214 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kumiko Sugiyama: "Stimulative effect of sodium nitroprusside on peristaltic reflex in isolated guinea pig ileal segment" Japanese Journal of Pharmacology. 57. 279-289 (1991)

    • Related Report
      1993 Annual Research Report
  • [Publications] Seiichi Komori: "GTP-binding protein involvement in membrane currents evoked by carbachol and histamine in guinea-pig ileal muscle" Journal of Physiology. 450. 105-126 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Seiichi Komori: "Membrane potential and current responses to neurotensin in the longitudinal muscle of the rectum of the fowl" British Journal of Pharmacology. 107. 790-796 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Hidenori Ohashi: "Neurotensin as a candidate for the excitatory transmitter of the non-adrenergic and non-cholinergic nerves in chicken rectum" Symposium on Autonomic Neuroeffector Mechanism:August 1993 8-11. (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Hidenori Ohashi: "Mechanical and current responses to neurotensin in the smooth muscle of guinea-pig intestine" Journal of Autonomic Pharmacology. 14. 239-251 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Seiichi Komori: "Potentiation by neurotensin of carbachol-induced tension development Ca2+-free solution in skinned guinea-pig ileal muscle" Japanese Journal of Pharmacology. 64. (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kumiko Sugusawa: "Stimulative effect of sodium nitroprusside on peristaltic reflex in isolated quineapig ileal segment" Japan.J.Pharmacol.57. 279-289 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] Seiichi Komori: "GTP-binding protein involvement in membrane currents evoked by carbachol and histamine in guinea-Pig ileal muscle" J.Physiol.450. 105-126 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Seiichi Komori: "Membrane potential and current responses to neurotensin in the longitudinal muscle of the rectum of the fowl" Brit.J.Pharmacol.107. 790-796 (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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