Inhibitory Effects of a Sustained Release Formulation of the Gonadotrophin-Releasing Hormone Agonist on Reproductive Function in the Dog
| Project/Area Number |
03454112
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied veterinary science
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| Research Institution | University of Osaka Prefecture |
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Principal Investigator |
MORI Junichi Univ.of Osaka Pref., Coll.of Agricul., Professor, 農学部, 教授 (90167685)
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| Co-Investigator(Kenkyū-buntansha) |
TAMADA Hiromichi Univ.of Osaka Pref., Coll.of Agricul., Pesearch Associate, 農学部, 助手 (10155252)
INABA Toshio Univ.of Osaka Pref., Coll.of Agricul., Assistant Professor, 農学部, 講師 (00137241)
SAWADA Tsutomu Univ.of Osaka Pref., Coll.of Agricul., Associate Professor, 農学部, 助教授 (60081600)
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| Project Period (FY) |
1991 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1993: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1992: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1991: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | Dog / Gonadotrophin-Releasing Hormone / Chemical Contraception / Hypothalamus / Anterior Pituitary / Luteinizing Hormone Receptor / Sex Hormones / Oestrus Induction / 性腺刺激ホルモン放出ホルモン / 黄体形成ホルモン / プロジェステロン / エストロジェン / アンドロジェン / 黄体形成ホルモン・レセプタ- / 性成熟 |
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| Research Abstract |
Inhibitory effects of a sustained release formulation of the gonadotrophin-releasing hormone agonist (GnRH-A) on reproductive function in the dog were examined, and the following results were obtained.
1. A Sensitive and specific enzyme immunoassay for the determination of GnRH using a monoclonal antibody has been developed. This method was successfully applied for analysis of hypothalamic and plasma GnRH.In male dogs, hypothalamic GnRH levels increased with age up to 4 months, then fell to a plateau from 6 months to 2 years. The time required for a 50% reduction in plasma levels following intravenous administation of synthetic GnRH to 5 adult male dogs was 2.2(]SY.+-。[)0.1 (SEM) min.
2. The development of testicular luteinizing hormone (LH) receptors in the dog was coincident with the increase in testicular growth and plasma androgens. The increase in gonadal sensitivity to LH could be reponsible for the marked increase in secretion of androgen which occurs during puberty in the male dog.
3. A single injection of sustained-release formulation of GnRH-A (1 mg peptide/kg) could inhibit pituitary-testicular function in the male dog. Four months after the treatment, a complete return to normal spermatogenesis was observed. When exposed to a 4-h continuous infusion of GnRH (10 nM), pituitary desnsitization was observed in in vitro perifusion experiments, suggesting that the long-acting potency of the drug could be the reason for the inhibition of the pituitary and testicular function.
4. During 9 months of treatment with multiple injections of sustained-release formulation of GnRH-A (1 mg peptide/kg, once every 3 months), starting within 4 months postpartum in bitches, no new oestrus was detected. On the contrary, a single injection of sustained-release formulation of GnRH-A (0.1 mg peptide/kg) at 5 months postpartum in bitches resulted in an induced heat : the bitches mated at those time and produced normal litters.
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Report
(4 results)
Research Products
(3 results)
