• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Electropharmacological analysis of the signal transduction mechanisms of the delayd rectifier potassium channel of heart cells.

Research Project

Project/Area Number 03454141
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field General pharmacology
Research InstitutionAkita University

Principal Investigator

IIJIMA Toshihiko  Akita University, Pharmacology, Professor, 医学部, 教授 (30004724)

Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥5,900,000 (Direct Cost: ¥5,900,000)
Fiscal Year 1992: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1991: ¥5,200,000 (Direct Cost: ¥5,200,000)
KeywordsVentricular cells / Patch-clamp method / Adenylate cyclase / Forskolin derivative / Delayd rectifier K^+ current / L-type Ca^<2+> current / Aortic endothelial cell / intracellular Ca^<2+> / 単離心室筋細胞 / 細胞内情報伝達機構 / L型カルシウムイオンチャンネル / 遅延整流カリウムイオンチャンネル / フォルスコリン / 血管内皮細胞 / モルモット心室筋細胞 / 遅延整流カリウムイオンチャンネル電流 / アデニル酸シクラ-ゼ / ベ-タアドレナリン受容体
Research Abstract

This study was designed to investigate the differential modulation of the L-type Ca^<2+> (I_<Ca>) and the delayd rectifier K^+ current (I_K) by direct activation of adenylate cyclase in guinea pig ventricular cells. Isoproterenol equally and simultaneously increased both I_<Ca> and I_K in a same threshold concentration, but a water soluble forskolin derivative, NKH477, increased I_K at lower threshold concentrations than that for increasing I_<Ca>. Selective stimulation of beta-1 adrenoceptors, on the other hand, elicited an increase in I_<Ca> more sensitively than I_K. These results suggest that a simple signal transduction pathway from beta adrenoceptors to I_<Ca> and I_K channels mediated through adenylate cyclase and cyclic AMP-dependent protein kinase is not sufficient to explain these differences. It is, therefore, concluded that I_<Ca> and I_K channels could be differentially regulated during beta adrenoceptor stimulation.
Mechanisms responsible for the sustained elevation in intracellular free Ca^<2+> concentration ([Ca^<2+>]_i) in response to histamine and ATP were also studied in cultured human aortic endothelial cells loaded with the fluorescent Ca^<2+> indicator fura-2. Results indicate that the sustained elevation of [Ca^<2+>]_i is resulted from the Cl^--sensitive entry of extracellular Ca^<2+> in human aortic endothelial cells.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (7 results)

All Other

All Publications (7 results)

  • [Publications] Harada,K.: "Differential modulation be adenylate cyclase of Ca^<2+> and delayed K^+ current in ventricular myocytes." American Journal of Physioloqy(Heart and Circulatory Physioloqy). 266. in press (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Hosoki,E.: "Chloride-sensitive Ca^<2+> entry by histamine and ATP in human aortic endothelial cells." European Journal of Pharmacoloqy(Molecular pharmacoloqy Section). 266. 213-218 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] K.Harada & T.Iijima: "Differential modulation by adenylate cyclase of Ca^<2+> and delayd K^+ current in ventricular myocytes." Am.J.Physiol. in press. (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] E.Hosoki & T.Iijima: "Chloride-sensitive Ca^<2+> entry by histamine and ATP in human aortic endothelial cells." Eur.J.Pharmacol. (Mol.Pharmacol. Sec.). 266. 213-218 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] K.Harada and T.Iijima: "Differential modulation by adenylate cyclase of Ca2^+ and delayed K^+ current in ventricular myocytes."

    • Related Report
      1992 Annual Research Report
  • [Publications] T.Iijima,M.Hosono and N.Taira: "Electropharmacological analysis of the forskolin binding site on adenylate cyclase in single ventricular cells of the guinea-pig heart."

    • Related Report
      1992 Annual Research Report
  • [Publications] E.Hosoki and T.Iijima: "Chloride-sensitive calcium entry by histamine and ATP in human aortic endothelial cells."

    • Related Report
      1992 Annual Research Report

URL: 

Published: 1991-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi