| Project/Area Number |
03454160
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Pathological medical chemistry
|
|---|
| Research Institution | Osaka University, Institute for Protein Research |
|---|
Principal Investigator |
HATANAKA Hiroshi Inst.Prot.Res./Prot.Biosyn./Prof., たんぱく質研究所, 教授 (60208519)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
IKEUCHI Toshihiko Inst.Prot.Res./Prot.Biosyn./Instr., 蛋白質研究所, 助手 (20093362)
|
|---|
| Project Period (FY) |
1991 – 1992
|
| Project Status |
Completed (Fiscal Year 1992)
|
| Budget Amount *help |
¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1992: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1991: ¥4,600,000 (Direct Cost: ¥4,600,000)
|
|---|
| Keywords | Nerve Growth Factor / Neurotrophic Factor / Brain-derived Neurotrophic Factor / Cell Culture / Differentiation / Neuronal Cell Survival / コリン作動性 |
|---|
| Research Abstract |
The development, survival and degeneration of neurons are regulated by numerous genetic and environmental factors. Since mature neurons are known not to undergo mitosis, it might be expected that factors such as nerve growth factor (NGF). NGF has previously been shown to play am important role in the neuronal differentiation of immature basal forebrain cholinergic neurons. We found now that NGF appears to support survival in culture of basal forebrain cholinergic neurons from postnatal rats. We now established a technique for culturing primary neuronal cells from upto 8-week-old rats. NGF induces differentiation of the neurons from neonatal as well as fetal ones. NGF does not induce the differentiation, but rather promotes the survival of these neurons from 2-week-old rats, in manner that is independent of plating cell density. Brain-derived neurotrophic factor (BDNF) gave the almost same effect as NGF.These neurons from postnatal 8-week-old rats, however, are less responsive to NGF. These results suggest that the physiological roles of NGF and BDNF change during the development of the rat brain.
|
