• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Effects of Nerve Growth Factor and its Family Proteins on the Survival of Cultured from Adult Rat Brains.

Research Project

Project/Area Number 03454160
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Pathological medical chemistry
Research InstitutionOsaka University, Institute for Protein Research

Principal Investigator

HATANAKA Hiroshi  Inst.Prot.Res./Prot.Biosyn./Prof., たんぱく質研究所, 教授 (60208519)

Co-Investigator(Kenkyū-buntansha) IKEUCHI Toshihiko  Inst.Prot.Res./Prot.Biosyn./Instr., 蛋白質研究所, 助手 (20093362)
Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1992: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1991: ¥4,600,000 (Direct Cost: ¥4,600,000)
KeywordsNerve Growth Factor / Neurotrophic Factor / Brain-derived Neurotrophic Factor / Cell Culture / Differentiation / Neuronal Cell Survival / コリン作動性
Research Abstract

The development, survival and degeneration of neurons are regulated by numerous genetic and environmental factors. Since mature neurons are known not to undergo mitosis, it might be expected that factors such as nerve growth factor (NGF). NGF has previously been shown to play am important role in the neuronal differentiation of immature basal forebrain cholinergic neurons. We found now that NGF appears to support survival in culture of basal forebrain cholinergic neurons from postnatal rats. We now established a technique for culturing primary neuronal cells from upto 8-week-old rats. NGF induces differentiation of the neurons from neonatal as well as fetal ones. NGF does not induce the differentiation, but rather promotes the survival of these neurons from 2-week-old rats, in manner that is independent of plating cell density. Brain-derived neurotrophic factor (BDNF) gave the almost same effect as NGF.These neurons from postnatal 8-week-old rats, however, are less responsive to NGF. These results suggest that the physiological roles of NGF and BDNF change during the development of the rat brain.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] Y.Kushima,T.Hama and H.Hatanaka,: "Interleukin-6 as a neurotrophic factor for promoting the survival of cultured catecholaminergic neurons in chemically defined medium from postnatal rat midbrains." Neutosci,Res.,. 13. 267-280 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Y.Enokido,Y.Akaneya,M.Niiobe,K.Mikoshiba and H.Hatanaka,: "Basic fibroblast growth factor rescues CNS neurons from cell death caused by high oxygen atmosphere in culture." Brain Res.,. 599. 261-271 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Y.Kushima and H.Hatanaka,: "Interleukin-6 and leukemia inhibitory factor promote the survival of acetylcholinesterase-positive neurons in culture from embryonic nat spinal cord." Neurosci,Lett.,. 143. 110-114 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.Nonomura and H.Hatanaka,: "Neurotrophic effect of beain-derived neurotrophic factor on basal forebrain cholinergic neurons in culture from postnatal rats." Neurosci,Res.,. 14. 226-233 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Y.Kushima,C.Nishio,T.Nonomura and H.Hatanaka,: "Effects of nerve growth factor and basic fibroblast growth factor on survival of cultured septal cholinergic neurons from adult rats." Brain Res.,. 598. 264-270 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] M.Kojima,N.Takahashi,T.Ikeuchi and H.Hatanaka,: "Nerve growth factor(NGF)-mediated up-regulation of lowaffinity NGF receptor gene expression in cultured basal forebrain cholinergic neurons from postnatal 3-day-old rats." Mol.Brain Res.,. 16. 267-273 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 畠中 寛: "「神課成長因子ものがたり」" 羊土社、東京, 134 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.Hama, Y.Kushima, M.Miyamoto, M.Kubota, N.Takei and H.Hatanaka: "Interleukin-6 improves the survival of mesencephalic catecholaminergic and septal cholinergic neurons from postnatal, two-week-old rats in cultures." Neurosci.40. 445-452 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Y.Kushima, T.Hama and H.Hatanaka: "Interleukin-6 as a neurotrophic factor for promoting the survival of cultured catecholaminergic neurons in chemically defined medium from postnatal rat midbrains." Neurosci. Res.13. 267-280 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Y.Enokido, Y.Akaneya, M.Niinobe, K.Mikoshiba and H.Hatanaka: "Basic fibroblast growth factor rescues CNS neurons from cell death caused by high oxygen atmosphere in culture." Brain Res.599. 261-271 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Y.Kushima and H.Hatanaka: "Interleukin-6 and leukemia inhibitory factor promote the survival of acetylcholinesterase-positive neurons in culture from embryonic rat spinal cord." Neurosci. Lett.143. 110-114 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.Nonomura and H.Hatanaka: "Neurotrophic effect of brain-derived neurotrophic factor on basal forebrain cholinergic neurons in culture from postnatal rats." Neurosci. Res.14. 226-233 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Y.Kushima, C.Nishio, T.Nonomura and H.Hatanaka: "Effects of nerve growth factor and basic fibroblast growth factor on survival of cultured septal cholinergic neurons from adult rats." Brain Res.598. 264-270 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] M.Kojima, N.Takahashi, T.Ikeuchi and H.Hatanaka: "Nerve growth factor(NGF)-mediated up-regulation of low-affinity NGF receptor gene expression in cultured basal forebrain cholinergic neurons from postnatal 3-day-old rats." Mol.Brain Res.16. 267-273 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Y.Kushima and H.Hatanaka: "Culture of neuronal cells from postnatal rat brain: Application to the study of neurotrophic factors." Prog. Neuro-Psycho pharmacol. & Biol. Psychiat.16. 617-633 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Y.Enokido and H.Hatanaka: In Neurotrophic Factors, Y.Tsukada and E.Shooter eds by, Jap.Sci.Soc.Press, Tokyo. Neurotrophic factors rescue neuronal cell death caused by oxygen toxicity in culture., 97-108 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Y.Kushima,T.Hama and H.Hatanaka,: "Interleukin-6 as a neurotrophic factor for promoting the survival of cultured catecholaminergic neurons in chemically defined medium from postnatal rat midbrains." Neurosci.Res.,. 13. 267-280 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Y.Enokido,Y.Akanoya,M.Niinobe,K.Mikoshiba and H.Hatanaka,: "Basic fibroblast growth factor rescues CNS neurons from cell death caused by high oxygen atmosphere in culture." Brain Res.,. 599. 261-271 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Y.Kushima and H.Hatanaka,: "Interleukin-6 and leukemia inhibitory factor promote the survival of acetylcholinesterase-positive neurons in culture from embryonic rat spinal cord." Neurosci.Lett.,. 143. 110-114 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] T.Nonomura and H.Hatanaka,: "Neurotrophic effect of brain-derived neurotrophic factor on basal forebrain cholinergic neurons in culture from postnatal rats." Neurosci.Res.,. 14. 226-233 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Y.Kushima,C.Nishio,T.Nonomura and H.Hatanaka,: "Effects of nerve growth factor and basic fibroblast growth factor on survival of culcured septal cholinergic neurons from adult rats." Brain Res.,. 598. 264-270 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] M.Kojima,N.Takahasi,T.Ikeuchi and H.Hatanaka,: "Nerve growth factor(NGF)-mediated up-regulation of low-affinity NGF receptor gene expression in cultured basal forebrain cholinergic neurons from postnatal 3 day old rats." Mol.Brain Res.,. 16. 267-273 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 畠中 寛: "「神経成長因子ものがたり」" 羊土社、東京, 134 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] T.Hama: "Interleukinー6 improves the survival of mesencephalic catecholaminergic and septal cholinergic neurons from postnatal,twoーweekーold rats in cultures." Neuroscience,. 40,. 455-452. (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] T.Motoike: "Expression and localization of amg p25A,a ras p21ーlike small GTPーbinding protein,in cultured rat hippocampal cells." Neursci.Lett.,. 134,. 100-112. (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Y.Kushima: "Culture of neuronal cells from postnatal rat brain:Application to the study of neurotrophic factors." Prog.NeuroーPsychopharmacol.& Biol.Psychiat.,. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 池内 俊彦: "神経成長因子(NGF)作用の分子機構" 蛋白質核酸酵素、. 36,. 1211-1219. (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 畠中 寛、: "神経細胞死と神経栄養因子ー神経細胞死概論としてー" 代謝、. 28、. 891-899. (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 榎戸 靖: "酸素毒性と神経細胞死ー培養中枢ニュ-ロンとPC12h細胞を用いたモデル系での解析ー" 代謝、. 28,. 923-931. (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 畠中 寛: "神経成長因子ものがたり" 羊土社、東京, (1992)

    • Related Report
      1991 Annual Research Report

URL: 

Published: 1991-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi