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Molecular-Pathological investigation of human thyroid cancer with a special focus on poorly differentiated carcinoma

Research Project

Project/Area Number 03454166
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Human pathology
Research InstitutionUniversity of Tokyo

Principal Investigator

SAKAMOTO Atsuhiko  Tokyo Univ.Fac.of Med.Dept.of Pathol.Associate Professor, 医学部(医), 助教授 (50215654)

Co-Investigator(Kenkyū-buntansha) ODA H.  Tokyo Univ.Fac.of Med.Dept.of Pathol.Instructor, 医学部, 助手 (40214142)
佐藤 明  東京大学, 医学部, 助手
MORI M.  Tokyo Univ.Fac.of Med.Dept.of Pathol.Instructor, 医学部, 助手 (90210137)
SUGIMURA H.  Hamamatsu Univ.School of Medicine, Dept.of Pathol.Associate Professor, 医学部, 助教授 (00196742)
DOBASHI Y.  Osaka Univ.Research Institute for amicrobial Diseases, Oncogene Research, Resear, 微生物病研究所, 研究員 (90231456)
SATO A.  Tokyo Univ.Fac.of Med.Dept.of Pathol.Instructor
Project Period (FY) 1991 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 1993: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1992: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1991: ¥3,300,000 (Direct Cost: ¥3,300,000)
KeywordsThyroid cancer / Poorly differentiated carcinoma / Immunohistochemistry / p53 oncogene / 染色体
Research Abstract

The Spectrum of phenotypic as well as genotypic variations in human thyroid carcinoma were investigated with respect to histopathological classification. In particular, the entity of "poorly differentiated carcinoma (PDC)" which we have previously proposed was tried to be established from biological viewpoint. In the immunohistochemical analysis, for 110 cases of thyroid carcinoma, we reconfirmed the consensus that thyroglobulin and keratin are more frequently expressed in well differentiated carcinoma(WDC) rather than undifferentiated carcinoma(UDC). To the contrary, PCNA, c-erb-B-2, p53 protein were found out to be more frequently overexpressed in UDC.In addition, PDC revealed the intermediate values in terms of the frequency of protein expression listed above.
In chromosomal analysis which was performed in 8 cases of papillary carcinoma, no specific mutation was identified.
For molecular pathological analysis, point mutation of p53 gene between exon 5 to 8 was investigated in each his … More tological type by utilizing SSCP and direct sequencing for the cases which were determined to be positive for p53 protein on immunohistochemistry. In 7 cases of WDC, no mutation was identified either in SSCP or in sequencing. In 6 cases of PDC, 2 cases revealed mutations and both were turned out to be one base substitution. In 6 cases of UDC, 4 cases revealed alteration in SSCP, and sequencing showed one-base substitution in 3 cases and two-base insertion in one case.
In summary, we have got involved the project targetting the phenotypic and genotypic characterization of thyroid carcinoma in line with histopathological classification, in significant number of the cases. There are definite differences between WDC and UDC in phonotype as well as genotype.In that sense, PDC exists as an intermediate entity of those two categories. Here, we demonstrated the distict entity of PDC during the process of dedifferentiation from WDC to UDC which is, clinicopathologically, the widely known phenomenon in human thyroid carcinoma. Less

Report

(4 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • 1991 Annual Research Report
  • Research Products

    (31 results)

All Other

All Publications (31 results)

  • [Publications] 坂本穆彦、森正也、他: "甲状腺乳頭癌の組織学的細分類とリンパ節転移" 癌の臨床. 37. 101-105 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 森正也、坂本穆彦、他: "甲状腺乳頭癌follicular variantの臨床病理学的検討" 癌の臨床. 38. 971-975 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Dobashi,Y.,Sakamoto,A.,et al.: "Overexpression of p53 as a possible prognostic factor in human thyroid carcinoma" American Journal of Surgical Pathology. 17. 375-381 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Yamashita,H.,Sakamoto,A.,et al.: "DNA ploidy and stromal bone formation as prognostic indicators of thyroid papillary carcinoma" Acta Pathologica Japonica. 43. 22-27 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Oyama,T.,Sakamoto,A.,et al.: "Encapsulated papillary carcinoma of the thyroid gland" Acta Pathologica Japonica. 43. 516-521 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Dobashi,Y.,Sakamoto,A.,et al.: "Stepwise participation of p53 gene mutation during dedifferentiation of human thyroid carcinomas" Diagnostic Molecular Pathology. 3(in press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 坂本穆彦: "腫瘍鑑別診断アトラス・甲状腺" 文光堂, (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 坂本穆彦(分担執筆): "最新内科学体系、内分泌疾患、甲状腺疾患" 中山書店, (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Sakamoto, A., Mori, M., et al.: "Relation of histological subclassification and lymph node metastasis of thyroid papillary carcinoma" Japanese Journal of Cancer Clinic. 37. 101-105 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Mori, M., Sakamoto, A., et al.: "A Clinicopathological study of papillary carcinoma, follicular variant of the thyroid" Japanese Journal of Cancer Clinic. 38. 971-975 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Dobashi, Y., Sakamoto.A., Sugimura.H.et al.: "Overexpression of p53 as a possible prognostic factor in human thyroid carcinoma" American Journal of Surgical Pathology. 17. 375-381 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Yamashita, H., Sakamoto, A.et al.: "DNA ploidy and stromall bone formation as prognostic indicators of thyroid papillary carcinoma" Acta Pathologica Japonica. 43. 22-27 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Oyama, T., Sakamoto, A., et al.: "Encapsulated papillary carcinoma of the thyroid gland" Acta Pathologica Japonica. 43. 516-521 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Dobashi, Y., Sakamoto, A., Sugimura, H., et al.: "Stepwise participation of p53 gene mutation during dedifferentiation of human thyroid carcinomas" Diagnostic Molecular Pathology. 3(in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Dobashi,Y.,Sakamoto,A.et al.: "Overexpression of p53 as a possible prognostic factor in human thyroid carcinoma" American Journal of Surgical Pathology. 17. 375-381 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yamashita,H.,Sakamoto,A.et al.: "DNA ploidy and stromal bone formation as prognostic indicators of thyroid papillary carcinoma" Acta Pathologica Japonica. 43. 22-27 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Oyama,T.,Sakamoto,A.et al.: "Encapsulated papillary carcinoma of the thyroid gland" Acta Pathologica Japonica. 43. 516-521 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Dobashi,Y.,Sakamoto,A.et al.: "Stepwise participation of p53 gene mutation during dedifferentiation of human thyroid carciomas" Diagnostic Molecular Pathology. 3(in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 坂本穆彦: "甲状腺穿刺吸引細胞診の現状と問題点" 日本臨床細胞学会雑誌. 32. 1079-1085 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 坂本穆彦: "甲状腺腫瘍の病理診断" 内分泌の進歩. 11. 93-101 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yamashita,H.,Sakamoto,A.et al.: "DNA Ploidy and stromal bone formation as Prognostic indicators of thyroid papillary carcinoma in aged Patients" Acta Pathologica Japonica. 43. 22-27 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 森 正也、坂本 穆彦、他: "甲状腺乳頭癌follicular variantの臨床病理学的検討" 癌の臨床. 38. 971-975 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 森 正也: "病理組織診断における電子顕微鏡の有用性" 病理と臨床. 10. 154-155 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Dobashi,Y.,Sakamoto,A.,et al.: "p53 as a possibie prognostic marker in human thyroid carcinoma" Am.J.Surg.Pathol.(1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 坂本 穆彦,森 正也,馬場 紀行,高橋 かほる,山崎 善弥: "甲状腺乳頭癌の組織学的細分類とリンパ節転移" 癌の臨床. 37. 101-105 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 坂本 穆彦: "甲状腺癌の病理診断" 病理と臨床. 9. 485-486 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 土橋 洋,柴田 龍弘,川淵 紅代: "パラフィン切片からのDNA抽出" 検査と技術. 20. 57-62 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Dobashi Y,Stern D.F.: "Membraneーanchored forms of EGF stimulate focus formation and intercellular communication" Oncogene. 6. 1151-1159 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 森 正也,坂本 穆彦: "甲状腺乳頭癌濾胞亜型の免疫組織化学的検討" 癌の臨床. 38. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Mernyei M,Sakamoto A: "An immunohistochemical evaluation of histological grading of thyroid cancer" Virchows Archives A.

    • Related Report
      1991 Annual Research Report
  • [Publications] 坂本 穆彦: "腫瘍鑑別診断アトラス・甲状腺" 文光堂, 174 (1991)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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