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Developmental Research on Preservation of Liver Graft by Improving the Hepatic Microcirculation

Research Project

Project/Area Number 03454231
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Gastroenterology
Research InstitutionJuntendo University School of Medicine

Principal Investigator

SATO Nobuhiro  Deaprtment of GE ,Juntendo Univ. Sch. Med. Prof., 医学部, 教授 (90028358)

Co-Investigator(Kenkyū-buntansha) TAKEI Yoshiyuki  1st Dept of Med, Osaka Univ Med Sch ,Physician, 医学部・附属病院, 医員
MIYAZAI Akihisa  Dept of GE Juntendo Univ Sch of Med Assistant, 医学部, 助手 (20200149)
YOKOI Yukio  Dept of GE Juntendo Univ Sch of Med Lecturer, 医学部, 講師 (20150633)
WATANABE Sumio  Dept of GE Juntendo Univ Sch of Med, Lecturer, 医学部, 講師 (20138225)
KITAMI Noriyuki  Dept of GE Juntendo Univ Sch of Med ,Assoc Prof, 医学部, 助教授 (90053256)
北村 庸雄  順天堂大学, 医学部, 助手 (20231285)
Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥6,600,000 (Direct Cost: ¥6,600,000)
Fiscal Year 1992: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1991: ¥5,600,000 (Direct Cost: ¥5,600,000)
KeywordsLiver Transplantation / Microcirculation / Preservation of Liver Graft / Cytoskeleton / Kupffer cells / Oxygenation / Ischeima-referfusion Injury / Ito cells / エンドセリン / 酸素飽和度 / レーザー顕微鏡 / 微小循環障害 / 白血球粘着 / グラフト障害 / 臓器反射スペクトル法
Research Abstract

In this project, we investigated (1)the role of hepatic microcirculation on the re-perfusion injury and (2)the preservation condition for the graft in liver transplantation.
The mechanisms to control sinusoidal blood flow were investigated using cultured Ito cells and Kupffer cells which exist in sinusoid. Immunohistochemical staining revealed that contractile proteins such as actin, myosin and desmin were richly distributed in Ito cells. Addition of vasoactive substance, endothelin-1, caused the contraction of Ito cells and this contractile activity was thought to contribute on sinusoidal contraction and control of blood flow. Kupffer cells showed active phagocytic activity with the actomyosin system and Kupffer cells with ingested foreign substances might block the sinusoidal blood flow. The predictive factors for the re-perfusion injury was investigated and revealed that the oxygenation of the surface of the liver was a best available for prediction of the prognosis of liver transplantation.
Although, the UW solution is standard for the liver preservation, we developed a new solution called "Carolina Rinse solution" for the better preservation. The clinical trial to assess the benefit of this solution for the liver transplantation has begun in Mayo Clinic in USA.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Goto M,Sato N et al: "Hepatic tissue oxygenation as a perdictive indiealor of ischauia-reperfnir liver injury" Hepatolgy. 15. 432-437 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Oshita M,Sato N et al: "Ethanol-induod iasoconstuctin cousr focal bepatocellatr injury in the isolated perfusid not liver" Hepatolgy. 16. 1009-1013 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Hijioka T,Sato N et al: "Ethauol-indised disterbana of hepatic miuocirenlation and hepatic bypoxia" Biochem.pharmowl.41. 1551-1557 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Ono K,Soto N et al: "Fiber optic erfiutana speitupiutonetry upton for in vivo tisue diagwos" Spplied Opris. 30. 98-105 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Hirose M,Sato N et al: "pertussis-toxin -induced redistibution of certical octomyosin and intibiton of phayorytous in rat kupllecell" F.gastrocut.Hepatol.(1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 竹井 謙之,佐藤 信雄他: "肝類調壁細胞機能保存による臓器保存法の開発" 病態生理. 12. 146-149 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Ono K et al: "Fiber optic reflectance spectro-photometry system for in vivo tissue diagnosis" Applied Optics. 30. 98-105 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Sato N et al: "Endoscopic spectrophotometry" Photomedicine & Photobiology. 13. 11-13 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Hijioka T et al: "Ethanol-induced disturbance of hepatic microcirculation and hepatic hypoxia" Biochem Pharmacol. 40. 1551-1557 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Oshita M et al: "Ethanol-induced focal cell necrosis via microcirculatory disturbance in the perfused rat liver" Alcohol & Alcoholism. (Suppl 1). 317-320 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Goto M et al: "Hepatic tissue oxygenation as a predictive Indicator of ischemia-reperfusion liver injury" Hepatology. 15(3). 432-437 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Hirose M et al: "Pertussis toxin-induced distribution of cortical actomyosin and inhibition of phagocytosis in rat Kupffer cells" J Gastroenterol Hepatol.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Oshita M et al: "Ethanol-induced vasoconstriction causes focal hepatocellular injury in the isolated perfused rat liver" Hepatology. 16(4). 1007-1013 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Takei Y et al: "Leucocyte adhesion and cell death following orthotopic liver transplantation in the rat" Transplantation. 51. 959-965 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Gao W et al: "Carolina-rinse solution - a new strategy to increase survival time after orthotopic liver transplantation in the rat" Transplnatation. 52. 417-424 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Takei Y et al: "A developmental research on preservation of liver graft by improving hepatic microcirculation (in Japanese)" Pathophysiology. 12. 146-149 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Goto M. Sato N et al: "Hepatri tissue oxygenation as a predictive indicator of ischemic-reperfusia liver injury" Hepatology. 15. 432-437 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Oshita M. Sato N et al: "Ethanol-induced vasownstriction cause, focal hepato-cellular injury in the isolated rat liver." Hepatology. 16. 1007-1013 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Hirose M, Sato N el al: "Pertussis toxin-induced redistribution of cortical actomyosin and inhibition of phagocytosis in rat Kupffer cells" J.Gastroenter.Hepatol.(1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 竹井謙之,佐藤信紘 他: "肝類洞壁細胞機能保存による臓巻保存法の開発" 病態生理. 12. 146-149 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Hijioka T,Sato N,Matsumura T,et al: "Ethanolーindused disturbance of hepatic micrccirculation and hepatic hypoxia." Biochemical Pharmacology. 40. 1551-1557 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Watanabe S,Miyazaki A,Sato N,et al: "Myosin in hepatocytes is essential for bile canalicular constraction." Liver. 11. 185-189 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Takei Y,Marzi I,Gores GJ,et al: "Leukocyte adhesion and cell death following orthotopic liver transplantation in the rat." Transplantation. 51. 959-965 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Goto M,Kawano S,Yoshihara H,et al: "Hepatic tissue oxygenation as a predictive indicator for ischemiareperfusion liver injury." Hepatology. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Takei Y,Gao W,Hijioka T,et al: "Rinsing Liver grafts with warm Ringer's solution increase survival by improving hepatic microcirculation." Transplantation. 52. 225-230 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Takei Y,Goto M,Yoshihara H,et al: "Microcirculation annual 1991" Tsuchiya M,et al eds.(日本医学館), 220 (1991)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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