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A morphological and biochemical study of argyrophilic inclusions in multiple system atrophy

Research Project

Project/Area Number 03454237
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Neurology
Research InstitutionUniversity of Tsukuba

Principal Investigator

MIZUSAWA Hidehiro  Tsukuba Univ., Neurology, Assoc.Prof., 臨床医学系, 助教授 (30144091)

Co-Investigator(Kenkyū-buntansha) YOSHIZAWA Toshihiro  Tsukuba Univ., Neurology, Assist.Prof., 臨床医学系, 講師 (50212311)
Project Period (FY) 1991 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥6,200,000 (Direct Cost: ¥6,200,000)
Fiscal Year 1993: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1992: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1991: ¥2,500,000 (Direct Cost: ¥2,500,000)
KeywordsMultiple system atrophy / argyrophilic inclusion / oligodendroglia / ubiquitin / alphaB-cystallin / ferric iron / Cu / Zu SOD / oxidative stress / αBクリスタル / 3価鉄 / ZnSOD
Research Abstract

Clinicopathological study of 6 brains of multiple system atrophy (MSA) revealed the precise distribution of glial cytoplasmic inclusions (GCIs) and a characteristic, laminar astrogliosis in the primary motor cortex, where a decrease in number of small to medium-sized neurons was found for the first time by a morphometric analysis.
GCIs were clearly immunostained for not only ubiquitin but also ubiquitin C-terminal hydrolase and 20S-/ 26S-proteasomes. The finding indictes that the main process of formation and degradation of GCIs is via ATP-dependent, non-lysosomal pathway.
Since oligodendroglia are involved in iron metabolism and iron is related to free radical formation, we investigated iron, ferritin and superoxide dismutase (SOD). In the MSA motor cortex, ferric iron was detected in microglia and macrophages with immunocytochemical localization of ferritin and Cu/Zu SOD within GCIs. These facts suggest first that GCIs might be related to neural death via oxidative stress.
In biochemical analysis, GCIs were partially purified from the MAS brain by sieving with mesh and discontinuous sucrose density gradient centrifugation. GCIs were mainly collected in the 45% to 50% sucrose interface which contained 32KDa and 40KDa proteins. These proteins were recognized with both anti-ubiquitin and anti-alpha B-cystallin antibodies, and not found in the identical fraction from the control brain. This is the first biochemical demonstration of alpha B-cystallin which might be ubiquitinated, as a component of GCI.

Report

(4 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • 1991 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] Akihide Mochizuki,Hidehiro Mizusawa,et al: "Argentophilic intracytoplasmic inclusions in multiple system atrophy" J.Neurology. 239. 311-316 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Tsuneo Fujita,et al: "The cerebral cortical pathology of sporadic olivopontocerebellar atrophy" J.Neurological Science. 116(in press). 41-46 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 水澤英洋: "多系統萎縮症におけるグリア細胞質内封入体" 神経研究の進歩. 37. 656-664 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 水澤英洋: "Lewy小体様ヒアリン封入体" 脳と神経. 44. 515-523 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Tameko Kihira,Hidehiro Mizusawa,et al: "Lewy body-like inclusions in Onnf's nucleus from two cases of sporadic amyotrophic lateral sclerosis" J.Neurological Science. 115. 41-46 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hidehiro Mizusawa,et al: "Hyaline and skein-like inclusions in amyotrophic lateral sclerosis" Neuropathology. (in press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hidehiro Mizusawa,et al: "Progressive supranuclear palsy presenting with pure akinesia.Adv.Neurol.Vol.60,pp618-621" Raven Press, 773 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hidehiro Mizusawa,et al: "Skein-like and hyaline inclusions in anterior horn cells in motor neuron disease." Smith-Gordon(in press), (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Mochizuki A, Mizusawa H, Ohkoshi N, Yoshizawa K, Komatsuzaki Y, Inoue K, Kanazawa I: "Argentophilic intracytoplasmic inclusions in multiple system atrophy." J Neurol. 239. 311-316 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Fujita T, Doi M, Ogata T, Kanazawa I, Mizusawa H: "Cerebral cortical pathology of sporadic olivopontocerebellar atrophy." J Neurol Sci. 116. 41-46 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Mizusawa H: "Glial cytoplasmic inclusions in multiple system atrophy." Adv Neurol Res. 37. 656-664 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Mizusawa H: "Pure akinesia and progressive supranuclear palsy." Brain Nerve. 45. 113-118 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Mizusawa H, et al: "Skein-like inclusions in the anterior horn cells in motor neuron disease." J Neurol Sci. 105. 14-21 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Mizusawa H: "Lewy body-like hyaline inclusion." Brain Nerve. 44. 515-523 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kihira T, Mizusawa H, et al: "Lewy body-like inclusions in Onuf's nucleus from two cases of sporadic amyotrophic lateral sclerosis." J Neurol Sci. 115. 51-57 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Mizusawa H: "Hyaline and skein-like inclusions in amytrophic lateral sclerosis." Neuropathology. (in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Mizusawa H, et al: "Progressive supranuclear palsy presenting with pure akinesia. In Narabayshi H, Nagatsu T, Yanagisawa N, Mizuno Y eds, Parkinson's disease. From Basic Research to Treatment (Adv Neurol Vol 60)." Raven Press. 618-621 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Mizusawa H, et al: "Skein-like and hyaline inclusions in anterior horn cells in motor neuron disease. In Rose FC ed, Amyotrophic lateral sclerosis." Smith-Gordon. (in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Tameko Kihira,Hidehiro Mizusawa et al: "Lewy body-like inclusions in Onnf's uncleus from two cases of sporadic amyotrophic lateral sclrosis" J.Neurological Science. 115. 51-57 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Tsuneo Fujita et al: "The cerebral cortical pathology of sporadic olivopontocerebellar atrophy" J.Neurological Science. 116(in press). 41-46 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 水澤英洋: "多系統萎縮症におけるグリア細胞質内封入体" 神経研究の進歩. 37. 656-664 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Hidehiro Mizusawa et al: "Hyaline and skein like inclusions in amyotrophic lateral sclerosis" Neuropathology. (in press).

    • Related Report
      1993 Annual Research Report
  • [Publications] Hidehiro Mizusawa et al: "Progrrssive supranuclear palsy presenting with pure akinesia.Adv.Neurol Vol.60" Raven Press, 773(618-621) (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Hidehiro Mizusawa et al: "Skein-like and hyaline inclusions in anterior horn cells in motor neuron disease." Smith-Gordon(in press), (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Akihide Mochizuki Hidehiro Mizusawa et al: "Argentophilic intracytoplasmic inclusions in multiple system atrophy" J.Neurology. 239. 311-316 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Tsuneo Fujita et al: "The cerebral cortical pathology of sporadic olivopontocerebellar atrophy" J.Neurological Science.

    • Related Report
      1992 Annual Research Report
  • [Publications] Akihide Mochizuki;Hidehiro Mizusawa;et al: "Argentophilic intracytoplasmic inclusions in multiple system atrophy" J.Neurology.

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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