| Project/Area Number |
03454251
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Nagoya University |
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Principal Investigator |
ASAI Kanichi (1992-1993) Nagoya University, School of Medicine Assistant Prof., 医学部, 講師 (70151008)
葛谷 文男 (1991) 名古屋大学, 医学部, 教授 (80023761)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIMINE Noboru Nagoya University, School of Medicine Associate Prof., 医学部, 助教授 (40022842)
NAITO Michitaka Nagoya University, School of Medicine Research Associate, 医学部, 助手 (10198012)
浅井 幹一 名古屋大学, 医学部, 講師 (70151008)
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| Project Period (FY) |
1991 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥5,800,000 (Direct Cost: ¥5,800,000)
Fiscal Year 1993: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1992: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1991: ¥2,900,000 (Direct Cost: ¥2,900,000)
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| Keywords | atherogenesis / endothelial cell / oxidatively modified lipoprotein / lipid hydroperoxide / copper ion / cytotoxity / endothelium-dependent relaxation / lysolecithin / 血管内皮 / 細胞障害性 / マクロファージ |
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| Research Abstract |
We studied the mechanism of the toxicity of oxidatively-modified LDL (oxLDL) modified by the presence of copper or iron against cultured bovine aortic endothelial cells. The cytotoxicity of the substances produced by the oxidation of LDL are present in oxLDL particles, but not in the apueous phase. The binding of copper ions to the oxLDL particle is required to induce toxicity in endothelial cells. The toxicity of oxLDL depends on the levels of LPO and not on the TBARS content, the extent of the negative charge, or the protein adduct of aldehydes, suggesting that lipid hydroperoxides associated with the oxLDL particle constitute the toxic moiety of oxLDL.And the lipid hydroperoxides may generate hydroxyl and alkoxyl radicals in the presence of iron, probably supplied by intracellular iron stores in endothelial cells and exhibit toxicity to the cells. And the impairment of endothelium-dependent relaxation of swine coronary artery by oxLDL is also related to both LPO and copper ion associated with the lipoptotein particle.
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