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Studies on the behavior of free radicals as a neurotoxin in the central nervous system

Research Project

Project/Area Number 03454292
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Psychiatric science
Research InstitutionFukushima Medical College

Principal Investigator

TSUCHIHASHI Nobuaki  Fukushima Medical College Associate Professor, 医学部, 助教授 (20005474)

Co-Investigator(Kenkyū-buntansha) ONO Mitsuhiro  Yamagata University, Department of Engineering, Professor, 工学部, 教授 (60007010)
MORI Norio  Fukushima Medical College Associate Professor, 医学部, 講師 (00174376)
Project Period (FY) 1991 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥5,700,000 (Direct Cost: ¥5,700,000)
Fiscal Year 1993: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1992: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1991: ¥3,200,000 (Direct Cost: ¥3,200,000)
KeywordsIn vivo ESR / Nitroxide / Aging / Idebenon / Vitamin E / Active oxgens / Antioxidant / Micloelectrode / 電子スピン共鳴 / スーパーオキシドディスムターゼ / 酵素電極 / ス-パオキシドディスムタ-ゼ
Research Abstract

Free radicals have toxic effects to the central nervous system. To assess this effects, we studied the behavior of free radicals in the living biological system using novel methods.
(1)in vivo ESR study
Metabolism of a stable nitroxide radical, TEMPOL, in living rats was observed using an L-band ESR system. TEMPOL was repeatedly injected at a 5-min interval via tail vein. The metabolism was analyzed in terms of the half-lives of TEMPOL elimination from the rat. In control animals, repeated injection of TEMPOL caused gradual prolongation of the half-lives. On the ontrary for rats receiving oral idebenone (a therapeutic agent for cerebro-vascular diseases) or vitamin E for 2-4 weeks, the prolongation was not found.
In addition, analysis of the half-lives of TEMPOL in rats ranging from 4 to 16 weeks-old suggests a lower ability to eliminate radicals in older rats.
(2)developments of microelectrode for bioradicals
Nitric oxide(NO) is a important bioreguratory molecules. And it is assumed that NO also have toxic effects in the central nervous systems. The technology, however, is not available for direct analysis of the release and/or distributions of NO.We developed a microelectrode based on polymeric metalloporphyrin and carbon fibers. Using this electrode we have detected NO occuring in the rat brain by administratrion of NMDA.

Report

(4 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • 1991 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] 土橋宣昭: "臨床医のための医用画像診断-ESR-" 臨床画像. 7. 70-80 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 土橋宣昭: "ESR-CTの基礎と臨床応用" 新医療. 19. 39-42 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 松本清治: "生体外から投与したニトロキシドラジカルの代謝に与えるイデベノンの影響についてiL-バンドESR法による研究" 神経化学. 32. 540-541 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 森則夫: "精神分裂病の慢性化と活性酸素仮説" 臨床精神医学. 22. 615-624 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 中川公一: "かん流肝におけるニトロキシドラジカルの還元作用" 磁気共鳴と医学. (印刷中). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M.Ono: "A Surface-coil type resonator for in vivo ESR measurement" J.Magn.Reson.,Ser.B. (印刷中). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 土橋宣昭(分担): "バイオ電磁工学とその応用" フジテクノシステム, 15 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 土橋宣昭(分担): "バイオサイエンスESR" (株)広川書店(印刷中), (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Matsumoto, S.: "L-band ESR imaging of the dsitribution of the nitroxide radical aministered into a mouse or a rat." Jikikyoumei to Igaku. 2. 57-62 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Ogata, T.: "Developments of in vivo detection and imaging of free radicals by L-band electron spin resonance" Radiological Science. 34. 2-6 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Ohno, K.: "Advances in electron paramagnetic resonance imaging" Trends Phys.Chem.2. 79-97 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Matsumoto, S.: "Effects of idebenone on the metabolic fate of intravenously given nitroxide radical in rats ; a study by means of L-band ESR technique" Bull.Jpn.Neurochem.Soc.32. 540-541 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Mori, N.: "Oxygen radical hypothesis for pathobiological process of scchizophrenia" Rinshou Seishin Igaku. 22. 615-624 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Nakagawa, K.: "Rapid free radical reduction in perfused rat liver" Jikikyoumei to Igaku. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Ono, M.: "A surface-coil type resonator for in vivo ESR measurements" J.Magn.Reson.Ser.B. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Tsuchihashi, N.: Measurements of biological system by ESR (ed.Omori, T.). Bioelectromagnetics snd its applications, Fuji-Tekunoshisutemu, 465-480 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Tsuchihashi, N.: Frontier of ESR measurements--medical imaging (ed.Sakurai, H.). Bioscience ESR, Hirokawa-Shoten, (in press),

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 松本清治: "生体外から投与したニトロキシドラジカルの代謝に与えるイデベノンの影響について;L-バンドESR法による研究" 神経化学. 32. 540-541 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 森 則夫: "精神分裂病の慢性化と活性酸素仮説" 臨床精神医学. 22. 615-624 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 中川公一: "かん流肝におけるニトロキシドラジカルの還元作用" 磁気共鳴と医学. (印刷中). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] M.Ono: "A Surface-coil type resonator for in vivo ESR measurements" J.Magn.Reson.Ser.B. (印刷中). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 土橋宣昭: "バイオサイエンスESR" (株)広川書店,東京(印刷中), (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 土橋 宣昭.松本 清治: "ESR-CTの基礎と臨床応用" 新医療. 19. 39-42 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 土橋 宣昭(分担): "バイオ電磁工学とその応用" フジ・テクノシステム, 833 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 土橋 宣昭,松本 清治: "ESRーCTの原理と臨床応用" 新医療. 19. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 土橋 宣昭,石田 信一: "臨床医のための医用画像診断:ESR" 臨床画像. 7. 70-80 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 土橋 宣昭(分担執筆): "バイオサイエンスESR" 廣川書店, (1992)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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