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Electromicroscopic and histochemical analysis in metabolism of the fat emulsion synthesized with new phospholip

Research Project

Project/Area Number 03454308
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field General surgery
Research InstitutionWakayama Medical College

Principal Investigator

TANIGUCHI Katsutoshi  Wakayama Medical College, Associate Proffessor, 消化器外科, 助教授 (90106568)

Co-Investigator(Kenkyū-buntansha) MANIWA Yoshio  Wakayama Medical College, Associate Proffessor, 消化器外科, 講師 (50229598)
NAGAI Yugo  Wakayama Medical College, Associate Proffessor, 消化器外科, 講師 (00172495)
Project Period (FY) 1991 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥5,600,000 (Direct Cost: ¥5,600,000)
Fiscal Year 1993: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1992: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1991: ¥4,500,000 (Direct Cost: ¥4,500,000)
Keywordsphospholipids / fat emulsion / HPLC / phosphatidylglycerol / phosphatidylglucose
Research Abstract

We produced a stable fat emulsion with a amall particle diameter by new liquid crystal emulsification method using phosphatidylglycerol, phosphatidylpolyethyleneglycol, and phosphatidyglucose, which are anionic surfactants, as emulsifiers. Electrom microscopy of the metabolism of the new fat emulsion in the liver by fat particle fixation method showed rapid uptake of fat particles and metabolism in liver cells compared with the conventional emulsion. In addition, the phospholipids and neutral fat in this emulsion were separately labeled with trltium and their pharmscokinetics was evaluated. Not only the phospholipids but also neutral fat was rapidly incorporated into the liver and metabolized. The phospholipids as emulsifiers markedly affected the organ directivity of neutral fat. For separation and analysis of phospholipids with different properties according to the class, a HPLC system using the post column method was developed. This system allowed separation and quantification of 6 glyceropholipids such as phosphatidylglycerol. The disappearance rate of these emulsifiers from the blood was higher than that of conventional yolk lecithin, suggesting only slight accumulation in the body.
In terms of metabolism of fat emulsion, it os improtant to take into account not only the metabolism of neutral fat but also that of phospholipids. These phospholipids are rapidly metabolized in the body and may be useful as fat emulsifiers.

Report

(4 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • 1991 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] 馬庭芳朗,他: "新しい栄養素材としてのジグリセリドO/Wエマルションの開発とその代謝動態" 日本外科学会雑誌. 95(臨増). 131-131 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 馬庭芳朗,他: "ジグリセリドエマルションの開発と静注後の脂肪粒子の代謝動態" 外科と代謝・栄養. 27(3). 252-252 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 馬庭芳朗,他: "脂肪乳剤投与後の人工脂肪粒子の代謝動態" 外科と代謝・栄養. 26(5). 542-545 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 瀧藤克也,他: "新しいリン脂質を用いた脂肪乳剤の作成" JJPEN. 15. 179-183 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 瀧藤克也,他: "新しい乳化剤による臓器指向性脂肪乳剤の作成" 日本外科学会雑誌. 93. 220 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 佐原雅基: "新組成O/Wエマルションの経腸投与における有用性" 消化と吸収. 15. 76-78 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 馬庭 芳朗,他: "新しい栄養素材としてのジグリセリドO/Wエマルションの開発とその代謝動態" 日本外科学会雑誌. 95(臨増). 131-131 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 馬庭 芳朗,他: "ジグリセリドアマルションの開発と静注後の脂肪粒子の代謝動態" 外科と代謝・栄養. 27(3). 252-252 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 馬庭 芳朗,他: "脂肪乳剤投与後の人工脂肪粒子の代謝動態" 外科と代謝・栄養. 26(5). 542-545 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 瀧藤 克也,他: "新しいリン脂質を用いた脂肪乳剤の作成" JJPEN. 15. 179-183 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 瀧藤 克也,他: "新しい乳化剤による臓器指向性脂肪乳剤の作成" 日本外科学会雑誌. 93. 220- (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] 佐原 稚基: "新組成O/Wエマルションの経腸投与における有用性" 消化と吸収. 15. 76-78 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] 馬庭 芳朗: "脂肪乳剤投与後の人工脂肪粒子の体内動態" 外科と代謝・栄養. 26. 542-545 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 馬庭 芳朗: "脂肪乳剤粒子のderivery systemに関する新しい知見" 日本消化器外科学会雑誌. 25. 1526- (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 瀧藤 克也: "新しい乳化剤による臓器指向性脂肪乳剤の作成" 日本外科学会雑誌. 93. 220- (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 瀧藤 克也: "新しいリン脂質を用いた脂肪乳剤の作成" JJPEN. 15. 179-183 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 佐原 稚基: "新組成O/Wエマルションの経腸投与における有用性" 消化と吸収. 15. 76-78 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 馬庭 芳朗: "肝における脂肪乳剤の代謝に関する形態学的研究を可能とする新しい固定法と肝オ-トラジオグラフィ-の確立" 和歌山医学. 42. (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 馬庭 芳朗: "中鎖脂肪酸トリグリセリド乳剤静注時の肝における脂質代謝に関する電子顕微鏡学的研究" 外科と代謝・栄養. 25. 375-382 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 馬庭 芳朗: "新組成の静注用脂肪乳剤の開発と肝の電顕的検討からみた有用性" 日本外科学会雑誌. 92. 177 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 瀧藤 克也: "新しい乳化剤による脂肪乳剤の作成とその代謝に関する生化学的・形態学的研究" 和歌山医学. 42. (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 瀧藤 克也: "標識脂肪乳剤による脂肪乳剤構成成分の体内動態" 日本静脈・経腸栄養研究会誌. 7. 142-145 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 瀧藤 克也: "標識脂肪乳剤を用いた脂肪乳剤構成成分の体内動態の解明" 外科と代謝・栄養. 26. (1992)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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