| Project/Area Number |
03454328
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Thoracic surgery
|
|---|
| Research Institution | Asahikawa Medical College |
|---|
Principal Investigator |
KUBO Yoshihiko Asahikawa Medical College.Prof., 医学部, 教授 (70000990)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
YOSHIDA Hiroki Asahikawa Medical College,Research Associate, 医学部, 助手 (00240309)
TANAKA Kunio Asahikawa Medical College,Associate Prof., 医学部, 助教授 (20041840)
SASAJIMA Tadahiro Asahikawa Medical College,Associate Prof., 医学部, 助教授 (20109515)
|
|---|
| Project Period (FY) |
1991 – 1992
|
| Project Status |
Completed (Fiscal Year 1992)
|
| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 1992: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1991: ¥3,200,000 (Direct Cost: ¥3,200,000)
|
|---|
| Keywords | ENDOTHELIAL CELL / MAGNETIC FIELD / VEIN GRAFT STENOSIS / INTIMAL HYPERPLASIA / RE-ENDOTHELIALIZATION / ENDOTHELIAL CELL CULTURE / 磁石 / 細胞培養 / ガウスメ-タ- |
|---|
| Research Abstract |
Re-endothelialization (RE) of vein grafts completed 2 - 3 weeks after implantation, whereas the endothelial cell coverage was still incomplete at the site of intimal hyperplasia (IH). It is considered that the IH might be preventable when RE is accelerated in some way. The purpose of this study is to elusidate the effect of the magnetic field on the endothelial cell proliferation in vitro and in vivo.
The H-shape permanent magnet was made for the experiments using two poles with cobalt magnet of the rare earth element and the iron yoke. The cultured vascular endothelial cells were positioned at the center region of the air gap (7cm) of the magnet and exposed to a 0.05 T static magnetic field. Changes in the growth rate of the cultured endothelial cells were observed. We observed the accelerate effect of the magnetic field on the endothelial cell proliferation at 5 - 7 days. Based on the results, we also applied the magnet to the autogenous vein grafts which were implanted into the carotid arteries of 4 dogs during the early phase after surgery. The endothelial cell coverage rate in the control grafts was 70 % of the luminal surface of the graft 10 days after implantation, whereas that of the exposed grafts was 84.5 % on the average, but there was stasistically no significant difference. We had encuraging results, however, it is necessary further study to elucidate the optimal condition of exposure in an application for implanted vascular grafts.
|
