| Project/Area Number |
03454345
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
TANIGUCHI Ikuo Tokyo Medical and Dental University, Professor Medical Research Institute., 難治疾患研究所, 教授 (60014255)
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| Co-Investigator(Kenkyū-buntansha) |
HORIKAWA Junsei Tokyo Medical and Dental University, Associate Professor Medical Research Instit, 難治疾患研究所, 助教授 (50114781)
KUROIWA Toshihiko Tokyo Medical and Dental University, Associate Professor Medical Research Instit, 難治疾患研究所, 助教授 (80129832)
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| Project Period (FY) |
1991 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1992: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1991: ¥4,600,000 (Direct Cost: ¥4,600,000)
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| Keywords | Optical Imaging / Cerebral Ischemia / Auditory Cortex / Functional Organization / Neural Activity / Tonotopical Organization / Voltage-Sensitive Dye / 大脳皮質 / 光学的計測 / 多チャンネル同時計測 / 病理組織 |
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| Research Abstract |
The spatio-temporal pattern of sound-evoked neural activity in the mammalian auditory cortex was studied by optical recording with aid of voltage-sensitive dye (RH 795). This method was applied to observation of transient ischemic changes in the cortical activity. Changes in light intensity at 620 nm induced sounds at various frequency and pressure levels were recorded with a 12 x 12 array of photodiodes. The amplitude of the responses were displayed as sequential two-dimensional images. Tonotopical organization was found in the multi-fields of the auditory cortex. However, frequency representation through the functional organization was transient. The focal activity that began in the primary field propagated dorsally along the iso-frequency bands. In the rats auditory cortex, the optical signals induced by sounds were disappeared by 1 min after induction of ischemia. During the 30 min of ischemia, no components of the responses were observed. After recirculation the responses from 144 sites appeared again. However the pattern of the responses after the transient ischemia was significantly different from the control. Especially the later component did not recover to the preischemic level. Although functional organization appeared, but vulnerable regions were found within the organization where the optical responses ded not return to the control Our results suggest that the functional changes in the auditory cortex precede the post-ischemic neuronal death in those cerebral regions. In guinea pigs, there were some cases which showed an increase in the magnitude of optical responses from the cortex by transient ischemia. The optical recording method has the advantage of real-time imaging of two-dimensional cortical activity and will be used widely in the future.
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