| Project/Area Number |
03454359
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Nagoya University |
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Principal Investigator |
SATO Keiji Nagoya University, School of Medicine Associate Professor, 医学部, 講師 (20178726)
|
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| Co-Investigator(Kenkyū-buntansha) |
YAMAMURA Shigeki Nagoya University, School of Medicine Staff, 医学部, 医員
SUGIURA Hideshi Nagoya University, School of Medicine Staff, 医学部, 医員
伊藤 隆安 名古屋大学, 医学部・大学院
河村 守雄 名古屋大学, 医療技術短期大学部, 助教授 (30186150)
佐藤 智太郎 名古屋大学, 医学部, 医員
中西 啓介 名古屋大学, 医学部, 医院
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| Project Period (FY) |
1991 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 1993: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1992: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1991: ¥5,700,000 (Direct Cost: ¥5,700,000)
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| Keywords | Bone Morphogenetic Protein / Ligamentous attachment / Bony allograft / Heat-treatment / Surface-demineralization |
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| Research Abstract |
Among heat-treated bone, bone morphogenegic protein was most preserved in a condition of 70 c, 10 minutes. Under this condition, new cartilge and bone was induced in maximal quantiry. Tendon insertions onto allogeneic bone already treated with several kinds of heat and time condition were studied histologically and biochemically. New bone formation was observed in groups with allografts incubated at 70 c. Tensile strengths examination at graft-insertion revealed surface-demineralization increased their strength up to that of the non-heat-treated group. Heat treatment with 60-70 c for 10 hours preserved bone-inductive activity as indicated by an induction of the mRNAs for alkaline phosphatase, type 1 and type 2 collagens which roughly correlated with the histological changes and Ca content of the implanted tissues.
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