| Project/Area Number |
03454363
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Kobe University |
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Principal Investigator |
MIZUNO Kosaku School of Medicine, Professor, 医学部, 教授 (90030981)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUBARA Tsukasa Kobe University Hospital, Associated Professor, 医学部・附属病院, 講師 (80192342)
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| Project Period (FY) |
1991 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 1993: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1992: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1991: ¥5,100,000 (Direct Cost: ¥5,100,000)
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| Keywords | fracture hematoma / freeze-dried hematoma / culture / osteoinduction / osteoconduction / cytokine / Transforming Growth Factor-beta / Type I collagen / Hydroxy apatite / Basic Fibroblast Growth Factor / Agar gel / 骨折 / 高齢者 / 骨形成因子 / 組織培養 / 組織修復 / 血腫 / 動物実験 / 生体材料 / 骨折治癒過程 / 骨折血腫 / サイトカイン / 骨移植 |
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| Research Abstract |
This study was conducted to provide further evidence of the inherent osteogenic potential of the fracture hematoma. The fracture hematoma was separated into its cellular and extracellular elements. The hematoma cells were cultured to study bone formation by the cellular elements alone. Bone formation acceleration factor was added to the cultured hematoma cells. The cell responded to this stimulation by differentiation into chondrocytes.
Fracture hematoma was freeze-dried to study the presence of osteoinduction by the extracellular factors in the fracture hematoma. The freeze-dried fracture hematoma was packaged in methylmethacrylate pellets and within capsules of hydroxyapatite. These pellets and capsules in response to extracellular humoral factors from the freeze-dried fracture hematoma.
The results of experimental implantation of the cultured fracture hematoma cells revealed that these cells had the potential to differentiate into chondroblasts or osteoblasts when bone induction factors and bone acceleration factor was added to their madia. These extracellular humoral factors are known to be present in the fracture hematoma.
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