| Project/Area Number |
03454371
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
麻酔学
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| Research Institution | Niigata University |
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Principal Investigator |
FUKUDA Satoru Dept.Anesth., Niigata Univ.Sch.Med.Assoc.Prof., 医学部, 助教授 (30116751)
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| Co-Investigator(Kenkyū-buntansha) |
MISAO Tomita Dept.Anesth., Niigata Univ.Sch.Med.Instructor, 医学部, 助手 (60221438)
NAOSHI Fujiwara Dept.Anesth., Niigata Univ.Sch.Med.Assist.Prof., 医学部・附属病院, 講師 (70181419)
多賀 紀一郎 新潟大学, 附属病院, 助手 (00163329)
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| Project Period (FY) |
1991 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥5,800,000 (Direct Cost: ¥5,800,000)
Fiscal Year 1993: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1992: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1991: ¥2,900,000 (Direct Cost: ¥2,900,000)
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| Keywords | Hypoxia / Endothlium / Adventitia / Calcium / Vascular smooth muscle / アシド-シス / 血管内皮 |
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| Research Abstract |
We investigated the effects of endothelium, adventitia (nerve), and blood cells(white blood cells : WBC) on vascular tone during hypoxia or acidosis. Hypoxia contracted blood vessels. Removal of endothelium attenuated the hypoxia-induced contraction of blood vessels. Cyclooxygenase inhibitors attenuated the hypoxia-induced contraction of blood vessels, suggesting that cyclooxygenase related eicosanoids may be released from endothlium during hypoxia. In contrast, removal of endothlium and adventitia abolished the hypoxia-induced contraction. These findings syggest that adventitia as well as endothlium may contribute to the hypoxia-induced contraction. It was also found that acidosis attnuated the agonist-induced contraction in a different manner depending the type of agonists used, and that endothelium facilitated the attenuated action of acidosis on agonist-induced contraction. From the experiment concerning the effect of WBC on vascular tone, we found that WBC dilates vascular smooth muscle indirectly through endothlium. We investigated the substance released from endothelium using some kinds of antagonists. However, we could not detect the substance. It has been reported that shear stress on endothelium releases endothlium-derived relaxing substance(s). Applying these phenomenon to research in testing human endothelial function, we found that endothelium did not work well in some kind of disease. In summary we found that endothelium, adventitia and blood vessels regulate vascular tone through their reciprocal actions.
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