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Study of Anti-germ Cell Tumor Antibody in Testicular Tumor Cell Growth and its Evaluation for Clinical Application

Research Project

Project/Area Number 03454387
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Urology
Research InstitutionKobe University

Principal Investigator

KAMIDONO Sadao  Kobe University, Urology, Professor, 医学部, 教授 (30030935)

Co-Investigator(Kenkyū-buntansha) GOTOH Akinobu  Kobe University, Urology, Part-time Lecturer, 医学部, 非常勤講師
OGAWA Takayoshi  Kobe University, Urology, Part-time Lecturer, 医学部, 非常勤講師 (70160760)
MATSUMOTO Osamu  Kobe University, Urology, Part-time Lecturer, 医学部, 非常勤講師 (80165878)
ARAKAWA Soichi  Kobe University, Urology, Associate Professor, 医学部, 助教授 (70159490)
Project Period (FY) 1991 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1993: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1992: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1991: ¥3,400,000 (Direct Cost: ¥3,400,000)
KeywordsAnti-germ cell tumor antibody / Testicular tumor / Monoclonal antibody / モノクロ-ナル抗体 / パラフィンブロック
Research Abstract

A specific monoclonal antibody, 5G9, was obtained from germ cell and germ cell tumor by making paraffin-embedded blocks of human testicular seminoma and used as the immunogen. This antibody reacts with the substance in the stage-specific process of spermatogenesis in embryos and adult testicles. The result of this study show the analysis of the reaction to the antibody and its possible clinical application.
1. Biochemical studies of 5G9
Measurements by Western blot revealed this antibody to be a protein of the Ig M class with a molecular weight of 29KD.
2. Immunohistochemical studies of 5G9 and normal tissues
Testicular germ cell and primordial germ cell stained positive ; however, spermatocytes, spermatids, spermatozoa, leydig cell and sertoli cell stained negative.
3. Immunohistochemical studies of 5G9 and malignant tumor tissues
All specimens of typical seminoma, spermatocytic seminoma, embryonal carcinoma, and choriocarcinoma stained positive ; however, the positive rate for the latter two was higher than for the former two. Urological malignant tumors, such as yolk sac tumor, teratoma, malignant lymphoma etc., stained negative. Human testicular germ cell line ITO-II also stained positive.
4. Evaluation of 5G9 for clinical application in testicular diagnosis
The positive rate for azoospermia and oligozospermia was 80% and 79%, respectively. Two cases of oligozospermia which spermatozoon stained positive, but no significant difference was detected in the rate of stain. Idiopathic azoospermia showed a 79% negative rate.
5G9 in normal testicles is considered to stain spermatogonia positively, and to be useful in determining the condition around the basement membrane rather than in detecting the existence of spermatogonia adjacent to the basement membrane. It is therefore suggested that immunohistochemical studies using 5G9 could be useful in evaluating the level of the residual sperm-producing ability of both azzospermia and aoligozospermia.

Report

(4 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • 1991 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] 守殿 貞夫: "精巣-とくに男子不妊での解釈" 泌尿器外科. 4. 457-461 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Morito Sakaue et.al.: "Production and characterization of four monoclonal antibodies against human ovarian clear cell carcinoma" Oncol.(Life Sci.Adv.). 11. 59-63 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 岡田 弘 他: "精液中の白血球サブポピュレーションについて-免疫組織染色とフローサイトメトリーを用いた検討" 日泌尿会誌. 84. 350-354 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Akinobu Gotoh et.al.: "Common expression of parathyroid hormone-related protein and no correlation of calcium level in renal cell carcinoma" Cancer. 71. 2803-2806 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hiroyoshi Shimogaki et.al.: "Variable expression of hst-1,int-2 and parathyroid hormone-related protein in different historogical types of human testicular germ cell tumors." The Cancer Journal. 6. 81-86 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 水野 禄仁 他: "ヒト精巣セミノーマ由来細胞TCam-2の樹立" 日泌尿会誌. 84. 1211-1218 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Sadao Kamidono: "Testicular biopsy and male infertility" Japanese Journal of Urological Surgery. 4. 457-461 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Morito Sakaue et.al.: "Production and characterization of four monoclonal antibodies against human ovarian clear cell carcinoma" Oncol.(Life Sci.Adv.). 11. 59-63 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hiroshi Okada et.al.: "Subpopulation of leucocytes in the semen from fertile and inferitile men -immunohistochemical and Flowcytometric Analysis-" Jpn.J.Urol.84. 350-354 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Akinobu Gotoh et.al.: "Common expression of parathyroid hormon-related protein and no correlation of calcium level inrenal cell carcinoma" Cancer. 71. 2803-2806 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hiroyoshi Shimogaki et.al.: "Variable expression of hst-1, int-2, and parathyroid hormone-related protein in different historogical types of human testicular germ cell tumors" The Cancer Journal. 6. 81-86 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Yosihito Mizuno et.al.: "Establishment and characterization of a new human testicular germ cell tumor cell lene(TCam-2)" Jpn.J.Urol.84. 1211-1218 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 岡田弘他: "精液中の白血球サブポピュレーションについて-免疫組織染色とフローサイトメトリーを用いた検討" 日泌尿会誌. 84. 350-354 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Akinobu Gotoh et.al.: "Common expression of parathyroid hormone-related protein and no correlation of calcium level in renal cell carcinoma" Cancer. 71. 2803-2806 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Hiroyoshi Shimogaki et.al.: "Variable expression of hst-1,int-2 and parathyroid hormone-related protein in different histological types of human testicular germ cell tumors" The Cancer Journal. 6. 81-86 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 水野禄仁他: "ヒト精巣セミノーマ由来細胞TCam-2の樹立" 日泌尿会誌. 84. 1211-1218 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 後藤 章暢: "Common Expression of Parathyroid Hormore-Rilated Protein aml No Crrilator of Caluum Lael in Renal Cell Carcinoma" Cancer. 71. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Atushi Takenaka: "Testicular Germ Cell Common Antigen Defined by a Newly established Monoclonal Antibody 5G9" Cancer. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Akinobu GoToh: "Common Expression of Porathyroid Hormonarelated Protein(PTHrP)and No correletion of Calcium level in Renal Cell Corcinoma" Cancer. (1992)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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