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A sperm motility inhibitor of human seminal plasma

Research Project

Project/Area Number 03454390
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Urology
Research InstitutionSt.Marianna University School of Medicine

Principal Investigator

IWAMOTO Teruaki  St.Marianna Univ. Urology, Associate Professor, 医学部, 助教授 (60046117)

Co-Investigator(Kenkyū-buntansha) TANAKA Hiroki  St.Marianna Univ. Urology, Instructor, 医学部, 助手 (00217069)
Project Period (FY) 1991 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 1993: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1992: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1991: ¥3,800,000 (Direct Cost: ¥3,800,000)
KeywordsSeminal plasma sperm motility inhibitor / Demenbranated spermatozoa / Dynein ATPase / Dynein arm / Seminal plasma / Seminal vesicle fluid / Seminal vesicle / Astchenozoospermia / 精子細胞抽出液 / 精子 / dynein ATPase
Research Abstract

1. A seminal plasma sperm motility inhibitor (SPMI) from boar was purified. The molecular weight of SPMI in native conditions has been estimated at 50kD by molecular sieving, but polypeptides with molecular weights of 14,16 and 18kD were observed following SDS-PAGE in denanring condition. The observation that SPMI effects on motility of demenbranated spermatozoa are reversed by Mg.ATP and that SPMI inhibited bull dynein ATPase suggest that this protein blocks the motility of demenbranated spermarozoa by interfering with dynein arm function.
2. The origin of human SPMI was investigated SPMI was present in significant amounts only in seminal vesical fluids (SVF). The level of biological activity in this fluid was 13.6-fold higher than in seminal plasma (SP). However, the concentration of motility inhibitor, as measured by ELISA, using an antibody generated against the motility inhibitor purified from human SP, was only 1.54-fold higher than in SP.The data suggest that SPMI originates from SVF and that it is processed into a nine-fold less biologically active form when SVF is mixed with other accessory gland secretions at ejaculation.
3. The origin f boar SPMI was investigated. By immunohistochemical detection at the electron microscope level, SPMI appeared to be present only on the surface of the secretory cells in the seminal vesicles(SV). The data indicate that SPMI originates from single tissue, the SV, and suggest that only the mature form present on the luminal surface of the gland can react with the antibody generated from rabbits immunized with the serected form of SP.
4. SPMI biological assay in the SP from infertile patients was measured. No significant correlation was observed between SPMI activity and sperm motility. This data suggest that the poor motile sperm may be caused by high permeability of the sperm membrane to SPMI.

Report

(4 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • 1991 Annual Research Report

Research Products

(14 results)

All Other

All Publications (14 results)

  • [Publications] T.Iwamoto: "Origin of a sperm motility inhibitor from boar seminal plasma." Molecular Reproduction and Development. 36. 475-481 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 岩本晃明: "ヒト精漿中に存在する精子運動抑制因子の研究:男子不妊症患者の精漿中濃度について" 日本不妊学会雑誌. 38. 1-5 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Iwamoto: "Purification and characterization of a sperm motility-dynein ATPase inhibitor from boar seminal plasma." Molecular Reproduction and Development. 31. 55-62 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M.Luterman: "Origin of the human seminal plasma motility inhibitor within the reproductive tract." International Journal of Andrology. 14. 91-98 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Iwamoto: "Origin of a sperm motility inhibitor from boar seminal plasma." Mol. Reprod. Devel.36. 475-481 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Iwamoto: "Biological activity of seminal plasma motility inhibitor in infertile patients." Jpn. J.Fertil. Steril.38. 1-5 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Iwamoto: "Purification and characterization of a sperm motility dynein ATPase inhibitor from boar seminal plasma." Mol. Reprod. Devel.31. 55-62 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M.Luterman: "Origin of the human seminal plasma motility inhibitor within the reproductive tract." Int. J.Androl.14. 91-98 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Iwamoto: "Origin of a sperm motility inhibitcr from pacr seminal plasma" Molecular Reproduction And Development. 36. 475-481 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 岩本 晃明 田中 宏樹 他: "ヒト精漿中に存在する精子運動抑制因子の研究:精子不妊症患者の精漿中濃度について" 日本不妊学会誌. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Luterman M.;Iwamoto T.;Gagnon C.: "Origin of the human seminal plasma motility inhibitor within the reproductive tract" Int.J.Andrology. 14. 91-98 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Iwamoto T.;Gagnon C.et.al: "Purification and characterization of sperm motility-dynein ATPase inhibitor from boar seminal plasma" Molec.Reprod Develop. 31. 55-62 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 岩本 晃明,田中 宏樹 他: "精子無力症患者精漿中の精子運動抑制因子の濃度と精子運動率" 日本不妊学会誌.

    • Related Report
      1991 Annual Research Report
  • [Publications] Iwamoto T.;Tanaka H.;Gagnon C.: "Origin of the boar seminal plasma motility inhibitor within the reproductive tract"

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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