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Molecular mechanisms of FSH secretion induced by novel

Research Project

Project/Area Number 03454391
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Obstetrics and gynecology
Research InstitutionGunma University, School of Medicine

Principal Investigator

IBUKI Yoshito  nonsteroidal gonadal factors., 医学部, 教授 (40008256)

Co-Investigator(Kenkyū-buntansha) SHIMADA Osamu  群馬大学, 医学部, 助手 (80196477)
MINEGISHI Takashi  群馬大学, 医学部, 講師 (00209842)
HASEGAWA Yoshihisa  群馬大学, 医学部, 講師 (40092001)
Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥6,600,000 (Direct Cost: ¥6,600,000)
Fiscal Year 1992: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1991: ¥5,800,000 (Direct Cost: ¥5,800,000)
KeywordsRat granulosa cell / FSH receptor / Activin / Activin receptor / インヒビン / ゴナドトロピン / レセプタ- / 卵巣 / 下垂体
Research Abstract

Previous studies reported that activin alone increased FSH receptor levels by using receptor binding assay and various hormone regulated FSH receptor mRNA levels in cultures rat granulosa cells. In this study, we examined the effect of activin A on FSH receptor mRNA in cultured rat granulosa cells using a FSH receptor cRNA probe. Northern blot analysis with cRNA of FSH receptor detected mRNA transcripts of 5.5- and 2.4-kb. Both transcripts are identical to size which have been reported previously in our in vivo studies. Our data showed that activin alone stimulated FSH receptor mRNA transcripts as well as FSH receptor levels in a concentration-dependent manner. Previous studies reported that FSH may maintain its own receptor mRNA and various growth factors as epidermal growth factor(EGF), basic fibroblast growth factor(bFGF), and insulin-like growth factor-I(IGF-I) did not alter basal levels of FSH receptor mRNA but EGF or bFGF dose-dependently attenuated the stimulatory actions of FSH on FSH receptor mRNA levels. Therefore, our results indicated that the stimulatory action of activin on the levels of the FSH receptor is closely related to the stimulatory action of activin on FSH receptor mRNA levels, and compared with other growth factors, activin may play a important role in the induction of the FSH receptor.
To understand the molecular mechanism of activin action, we have cloned the rat type II activin receptor from a rat ovary cDNA library. This result revealed that the receptor intracellular domain was a serine/threonine-specific protein kinase. The results that the granulosa cells contained two predominant rat type II activin receptor mRNA transcripts (6.0 and 3.0 kilobases). Furthermore, in vivo experiments showed that the mRNA levels of activin receptor in immature rat ovary has been induced according to the maturation of follicles and even after ovulation it continued to increase.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] K.Sugini,T.Nakamura,K.Takio,K.Miyamoto,Y.Hasegawa,M.Igarashi: "Purification and characterization of high molecular weight forms of inhibin from bovine follicular fluid." Endocrinology. 130. 789-796 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.Nakamura,Y.Hasegawa,K.Sugino,K.Kogawa,K.Titani,H.Sugino: "Follistatin inhibits activin-induced differentiation of rat follicular granulosa cells in vitro." Biochimica et Biophysica Acta. 1135. 103-109 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] K.Andoh,H.Mizunuma,Y.Hasegawa,K.Yamada M.Michishita,Y.Ibuki: "Association of immunoreactive-inhibin levels with luteal function." Hormone Research. 37. 37-40 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.Takeuchi,M.Seki,Y.Hasegawa,K.Tsuchiya,M.Itoh,I.Itoh,H.Shinozaki: "Further observations on serial serum immunoreactive inhibin levels in the luteal phase and early gestation after ovarian stimulation for in vitro fertilization and embrio transfer." Hormone Research. 37. 69-74 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] H.Shinozaki,I.Itoh,Y.Hasegawa,K.Nakamura,S.Igarashi,M.Nakamura: "Cloning and sequencing of a rat type II activin receptor." FEBS letters. 312. 53-56 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] H.Kaneko,Y.Nakanishi,K.Taya,H.Kishi,G.Watanabe,S.Sasamoto,Y.Hasegawa: "Evidence that inhibin is an important factor in the regulation of FSH secretion during the mid-luteal phase in cows." Journal of Endocrinology. 136. 35-41 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.Minegishi, K.Nakamura, Y.Takakura, Y.Ibuki and M.Igarashi: "Cloning and sequencing human FSH receptor cDNA." Biochem Biophys Res Commun. 175. 1125-1130 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] K.Nakamura, T.Minegishi, Y.Takakura, K.Miyamoto, Y.Hasegawa Y.Ibuki and M.Igarashi: "Hormonal regulation of gonadotropin receptor mRNA in rat ovary doing follicular growth and luteinization." Molecular and Cellular Endocrinology. 82. 559-263 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] M.Doi, M.Igarashi, Y.Hasegawa, Y.Eto, H.Shibai, T.Miura and Y.Ibuki: "In vivo action of activin-A on pituitary-gonadal system." Endocrinology. 130. 139-144 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] K.Sugino, T.Nakamura, K.Takai, K.Miyamoto, Y.Hasegawa and I.Igarashi: "Purification and characterization of high molecular weight forms of inhibin from bovine follicular fluid." Endocrinology. 130. 789-796 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.Nakamura, Y.Hasegawa, K.Sugino, K.Kogawa, K.Titani and H.Sugino: "Follistatin inhibits activin-induced differentiation of rat follicular granulosa cells in vitro." Biochemica et Biophysica Acta. 1135. 103-109 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] K.Andoh, H.Mizunuma, Y.Hasegawa, K.Yamada, M.Michishita and Y.Ibuki: "Association of immunoreactive-inhibin levels with luteal function." Hormone Research. 37. 37-40 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.Takeuchi, M.Seki, Y.Hasegawa, K.Tsuchiya, M.Itoh, I.Itoh and H.Sinozaki: "Further observations on serial immunoreactive inhibin levels in the luteal phase and early gestation after ovarian stimulation for in vitro fertilization and embryo transfer." Hormone Research. 37. 69-74 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] H.Sinozaki, I.Itoh, Y.Hasegawa, K.Nakamura, S.Igarashi, M.Nakamura, K.Miyamoto, Y.Eto, Y.Ibuki and T.Minegishi: "Cloning and sequencing of a rat type II actin receptor." FEBS letters. 312. 53-56 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] K.Sugini,T.Nakamura K.Takio,K.Miyamoto,Y.Hasegawa,M.Igarashi: "Purification and characterization fo high molecular weight forms of inhibin from bovine follicular fluid." Endocrinology. 130. 789-796 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] T.Nakamura,Y.Hasegawa K.Sugino,K.Kogawa K.Titani,H.Sugino: "Follistatin inhibits activin-induced differentiation of rat follicular granulosa cells in vitro." Biochimica et Biophysica Acta. 1135. 103-109 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] K.Andoh,H.Mizunuma Y.Hasegawa,K.Yamada M,Michishita,Y.Ibuki: "Association of immunoreactive-inhibin levels with luteal function." Hormone Research. 37. 37-40 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] T,Takeuchi,M.Seki Y.Hasegawa,K.Tsuchiya M.Itoh,I.Itoh,H.Shinozaki: "Further observations on serial serum immunoreactive inhibin levels in the luteal phase and early gestation after ovarian stimulation for in vitro fertilization and embrio transfer." Hormone Research. 37. 69-74 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] H.Shinozaki,I.Itoh,Y.Hasegawa,K.Nakamura,S.Igarashi,M.Nakamura: "Cloning and sequencing of a rat type 2 activin receptor" FEBS letters. 312. 53-56 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] H,Kaneko,Y.Nakanishi,K.Taya,H.Kishi,G.Watanabe S.Sasamoto,Y.Hasegawa: "Evidence that inhibin is an important factor in the regulation of FSH secretion during the mid-luteal phase in cows." Journal of Endocrinology. 136. 35-41 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] M.Yamaguchi,H.Mizunuma,K.Miyamoto,Y.Hasegawa,Y.Ibuki and M.Igarashi: "Immunoreactive inhibin concentration in adult men:Presence of a circadian rhythm." J.of Clin.Endocrinol.and Metabol.72. 554-559 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] M.Haji,Y.Nishi,S.Tanaka,M.Ohashi,K.Sekuya,Y.Hasegawa,et al: "Evidence for the secretion of inhibinーlike immunoreactivity from cultured human adrenal cells." Journal of Endocrinology. 128. R13-R16 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] H.Kaneko,T.Terada,K.Taya,G.Watanabe,S.Sasamoto,Y.Hasegawa and M.Igarashi: "Ovarian follicular dynamics and concentrations of oestradiolー17β,progesterone,luteinizing hormone,follicle stimulating hormone during the periovulatory phase of oestrous cycle in the cow." Reproduction,Fertility and Development. 3. 529-535 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] K.Nakamura,T.Minegishi,Y.Takakura,K.Miyamoto,Y.Hasegawa,Y.Ibuki and M.Igarashi: "Hormornal regulation of gonadotropin receptor mRNA in the rat ovary during follicular grouth and luteinization." Molecular Cellular Endocrinology. 82. 259-263 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] M.Doi,M.Igarashi,Y.Hasegawa,Y.Eto,H.Shibai,T.Miura and Y.Ibuki: "In vivo action of ActivinーA on pituitaryーgonadal system." Endocrinology. 130. 139-144 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] K.Andoh,H.Mizunuma,Y.Hasegawa,K.Yamada,M.Michishita and Y.Ibuki: "Immunoreactive inhibin levels sa a marker of luteal function." Hormone Research. (1992)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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