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Endocrinological and immunological study on regulatory mechanism of human endometrial differentiation

Research Project

Project/Area Number 03454396
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Obstetrics and gynecology
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

KANZAKI Hideharu  Kyoto University, Faculty of Medicine Associate professor, 医学部, 助教授 (80135566)

Co-Investigator(Kenkyū-buntansha) TAKAKURA Kenji  Kyoto University, Faculty of Medicine Assistant Professor, 医学部, 助手 (10221350)
MAEDA Michiyuki  Kyoto University, Faculty of Medicine Associate Professor, 医学部, 助教授 (20027329)
Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1992: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1991: ¥3,800,000 (Direct Cost: ¥3,800,000)
Keywordshuman endometrium / decidualization / prolactin secretion / progesterone / M-CSF / LIF / androgen / peptidase antigens / ペプチダーゼ阻害剤 / インタ-ロイキン1 / MーCSF / モノクロ-ナル抗体 / ペプチダ-ゼ抗原
Research Abstract

Human endometrium was obtained from the patients with normal menstrual cycles who underwent hysterectomy for the treatment of gynecological diseases. The tissue samples were minsed and incubated in RPMI medium containing 10 % fetal bovine serum, collagenase and DNase. Glandular cells were separated from stromal cells by differential sedimentation. The stromal cells cultured with progesterone decidualized morphologically and secreted prolactin into the culture supernatant. Using this system, the effects of interleukin 1 (IL-1), Macrophage colony-stimulating factor (M-CSF), leukemia inhibitory factor (LIF) and testosterone on the endometrial differentiation were studied. Furthermore, endometrium was examined immunohistologically with monoclonal antibodies against peptidase antigens. The results were as follows:
1. IL-1 inhibited morphological decidualization of endometrial stromal cells in vitro, and also reduced prolactin secretion by the cultured endometrial stromal cells in a dose-dependent manner.
2. Cultured endometrial stromal cells secreted M-CSF in the presence of progesterone. The expression of M-CSF mRNA was enhanced by the addition of progesterone. Endometrial glandular cells also secreted M-CSF, but it was not under the sex steroidal control.
3. LIF was mainly detected on the endometrial glandualr cells.
4. Testosterone induced prolactin secretion by the cultured endometrial stromal cells in a dose dependent manner. This prolactin secretion was enhanced by the addition of progesterone.
5. Endometrial stromal cells expressed aminopeptidase N and neutral endopeptidase, whereas endometrial glandular cells expressed dipeptidyl peptidase IV. The expression of these peptidase antigens changed during menstrual cycle and pregnancy.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Kanzaki H el al.: "The in situ expression of GM-CSF mRNA at the materno-fetal interface" Growth Factors. 5. 69-74 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Okamoto N el al.: "Supression by human placental protein 14 of natural killer cell activity" American Journal of Reproductive Immunology. 26. 137-142 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kariya M el al.: "Interleukin-1 ingibits in vitro decidualization of human endometrial stromal cells" Journal of Clinical Endocrinology and Metabolism. 73. 1170-1174 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kanzaki H el al.: "The expression and localization of mRNA for colony-stimulationg factor(CSF)-1 in the human placenta" Human Reproduction. 7. 563-567 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Imai K et al.: "Human endometrial stromal cells and decidual cells express cluster of differentiation (CD) 13 antigen/aminopeptidae N and CD10 antigen/neutral endopeptidase" Biology of Reproduction. 46. 328-334 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Horie K et al.: "Expression of c-kit protein during placental development" Biology of Reproduction. 47. 614-620 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kanzaki H et al.: "The in situ expression of GM-CSF mRNA at the materno-fetal interface" Growth Factors. 5. 69-74 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kariya M et al.: "Interleukin-1 inhibits in vitro decidualization of human endometrial stromal cells" Journal of Clinical Endocrinology and Metabolism. 73. 1170-1174 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Okamoto N et al.: "Suppression by human placental protein 14 of natural killer cell activity" American Journal of Reproductive Immunology. 26. 137-142 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kanzaki H et al.: "The expression and localization of mRNA for colony-stimulating factor(CSF)-1 in the human placenta" Human Reproduction. 7. 563-567 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Imai K et al.: "Human endometrial stromal cells and decidual cells express cluster of differentiation (CD) 13 antigen/aminopeptidase N and CD10 antigen/neutral endopeptidase" Biology of Reproduction. 46. 328-334 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Horie K et al.: "Expression of c-kit protein during placental development" Biology of Reproduction. 47. 614-620 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Imai K et al.: "Dipeptidyl peptidase IV as a differentiation marker of human endometrial glandular cells" Human Reproduction. 7. 1189-1192 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Imai K et al.: "Expression of aminopeptidase N and neutral endopeptidase on the endometrial stromal cells in endometriosis and adenomyosis" Human Reproduction. 7. 1326-1328 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kanzaki H et al.: "The expression and localization of mRNA for colonystimulating factor(CSF)-1 in the human placenta" Human Reproduction. 7. 563-567 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Horie K et al.: "Expression of c-kit protein during placental development" Biology of Reproduction. 47. 614-620 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Imai K et al.: "Expression of aminopeptidase N and neutral endopeptidase on the endometrial stromal cells in endometriosis and adenomyosis" Human Reproduction. 7. 1326-1328 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Imai K et al.: "Human endometrial stromal cells and decidual cells express cluster of differentiation (CD)13 antigen/aminopeptidae N and CD10 antigen/neutral endopeptidase" Biology of Reproduction. 46. 328-334 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Imai K et al.: "Dipeptidyl peptidase IV as a differentiation marker of human endometrial glandular cells" Human Reproduction. 7. 1189-1192 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Kanzaki H et al.: "The in situ expression of GMーCSF mRNA at the maternalーfetal interface" Growth Factors. 5. 69-74 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kariya M et al.: "Interleukinー1 inhibits in nitro decidualization of human endometrial stromal cells" Journal of Clinical Endoclinology and Metabolism. 73. 1170-1174 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Imai K田et al.: "Human Endometrial Stomal Cells and Decidual Cells Express Cluster of Differentiation(CD)13 Antigen/Aminopeptidase N and CD10 Antigen/Neutral Endopeptidase" Biology of Reproduction.

    • Related Report
      1991 Annual Research Report
  • [Publications] Kanzaki H et al.: "The expression and localization of mRNA for colonyatimulating factor(CSF)ー1 in the human placenta" Human Reproduction.

    • Related Report
      1991 Annual Research Report
  • [Publications] Okamoto N et al.: "Phenotypic and functional analysis on large granular lymphocytes(LGL)in human decidua of early pregnancy"

    • Related Report
      1991 Annual Research Report
  • [Publications] Imai K et al.: "Dipeptidyl peptidase IV as a differentlation maker of human endometrial glandular cells"

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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