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Study of neurotransmitter in cochlea by patch-clamp methods

Research Project

Project/Area Number 03454406
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Otorhinolaryngology
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

KOMUNE Sizuo (1992-1993)  Medicine, Kyushu university, Assistant Professor, 医学部, 助教授 (10117434)

上村 卓也 (1991)  九州大学, 医学部, 教授 (00075201)

Co-Investigator(Kenkyū-buntansha) NAKAGAWA Takashi  Kyushu university, Medical staff, 医学部, 医員
KIMITSUKI Tkakashi  Medicine, Kyushu university, Research Associate, 医学部, 助手 (50240908)
小宗 静男  九州大学, 医学部, 講師 (10117434)
Project Period (FY) 1991 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1993: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1992: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1991: ¥4,700,000 (Direct Cost: ¥4,700,000)
KeywordsGuinea pig / Chick / Cochlea / Hair cell / Ganglion cell / Patch-clamp / Ion channel / Ototoxic drug / ひよこ / 単離細胞 / 外有毛細胞 / カリウム電流 / 静止膜電位
Research Abstract

We studied ion channels, which is exist on the membrane of cochlear hair cells and ganglin cells. These cells play an important role on neurotransmitter mechanism in cochlea. We usued patch-clamp methods. We investigated the effect of ototoxic drugs on mechano-electric transduction channels in chick cochlear hair cells. Cisplatin and Digydrostreptomycin blocked the mechano-electroc trarsduction channel. Two Cisplatin molecules or one dihydrostreptomycin molecule blocked one trarsduction channel. We studied the voltage-dependent ion channels in guinea big outer hair cells. There are two types of potassium channels, one of them is activated even at resting potential. Voltage-dependent calium channel is blocked by aminogycoside antibiotics. This channel was investigated by recording single channel on cell-atlanched mode. We cleared many types of ion channels in cochlear ganglion cells. There are three types of voltage-dependent channels, first the sodium channel, second the calcium channel, and the last the potassium channel. And the chemical receptors, which reponsed by glutamate, was existed on the memnrane of ganglion cells This suggested that the transmitter from the cochlear hair cells to the ganglion cells should be glutamate.

Report

(4 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • 1991 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] T.NAKAGAWA: "Calcium channel in isolated outer hair cells of guinea pig cochlea." Neuroscience Letters. 125. 81-84 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.NAKAGAWA: "Effect of Ca^<2+> antagonists and aminoglycoside antibiotics on Ca^<2+> current in isolated outer hair cells of guinea pig cachlea." Brain Research. 580. 345-347 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.NAKAGAWA: "voltage-dependent channels in dissociated outer hair cells of guinea pig." Proceeding of Sendai Symposium '92 on Cochlear Neurobiology. 1. 31-35 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.KIMITSUKI: "Dihydrostreptomycin modifies adaptation and blocks the mechano-electric transduction in chick cochlear hair cells." Brain Research. 624. 143-150 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.KIMITSUKI: "Cisplatin blocks mechano-electric transducer current in chick cochlear hair cells." Hearing Resesrch. 71. 64-68 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.KIMITSUKI: "Single channel recordings of calcium current in chick cochlear hair cells." Acta Oto-Laryngologica. (In press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.NAKAGAWA: "Calcium channel in isolated outer hair cells of guinea pig cochlea" Neuroscience letters. 125. 81-84 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.NAKAGAWA: "Effect of Ca^<2+> antagonists and aminoglycoside andibiotics on Ca^<2+> current in isolated outer hair cells of guinea pig cachlea" Brain Research. 580. 345-347 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.NAKAGAWA: "Voltage-dependent channels in sissociated outer hair cells of guinea pig" Proceeding of Sendai Symposium '92 on Cochlear Neurobiology. 1. 31-35 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.KIMITSUKI: "Digydrostreptomycin modifieds adaptation adn blocks the mechano-electric transduction in chick cochlear hair cells" Brain Research. 624. 143-150 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.KIMITSUKI: "Cisplatin blocks mechano-electric transducer current in chick cochlear hair cells" Haaring Research. 71. 64-68 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.KIMITSUKI: "Single channel recordings of calcium current in chick cochlear hair cells" Acta Oto-Laryngologica. in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.KIMITSUKI: "Dihydrostreptomycin modifies adaptation and blocks the mechano-electric transduction in chick hair cells." BRAIN RESEARCH. 624. 143-150 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] T.KIMITSUKI: "Cisplatin blocks mechano-electric transducer current in chick cochlear hair cells." HEARING RESEARCH. 71. 64-68 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] T.KIMITSUKI: "Ototoxic drugs block the mechano-electric transduction channels in chick cochlear hair cells." PROCEEDING OF SENDAI SYMPOSIUM. (IN PRESS).

    • Related Report
      1993 Annual Research Report
  • [Publications] T.KIMITSUKI: "Single channel recordings of calcium currents in chick cochlear hair cells." ACTA OTO-LARYNGOLOGICA. (IN PRESS).

    • Related Report
      1993 Annual Research Report
  • [Publications] T.NAKAGAWA: "EFFECTS OF CA2+ ANTAGONISTS AND AMINOGLYCOSIDE ANTIBIOTICS ON CA2+ CURRENT IN ISOLATED HAIR CELLS OF GUINEA PIG COCHLEA" BRAIN RESEARCH. 580. 345-347 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] T.NAKAGAWA: "VOLTAGE‐DEPENDENT CHANNELS IN DISSOCIATED OUTER HAIR CELLS OF GUINEA PIG" PROCEEDINGS OF SENDAI SYMPOSIUM'92 ON COCHLEAR NEUROBIOLOGY. 1. 31-35 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] T.NAKAGAWA: "IONIC PROPERTIES OF IK,N IN OUTER HAIR CELL OF GUINEA PIG" BRAIN RESEARCH(SUBMITTED).

    • Related Report
      1992 Annual Research Report
  • [Publications] T.NAKAGAWA: "EFFECTS OF田CA^<2+> ANTAGONISTS AND AMINOGLYCOSIDE ANTIBIOTICS ON CA^<2+> CURRENT IN ISOLATED OUTER HAIR CELLS OF GUINEA PIG COCHLEA" Brain Research.

    • Related Report
      1991 Annual Research Report
  • [Publications] T.NAKAGAWA: "POTASSIUM CONDUCTANCE ACTIVATED AT RESTING MEMBRANE POTENTIAL OF OUTER HAIR CELL OF GUINEA PIG COCHLEA" Journal of Physiology.

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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