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Cloning of proteins produced by osteoblastic cells in response to 1alpha,25(OH)_2D_3.

Research Project

Project/Area Number 03454436
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Functional basic dentistry
Research InstitutionShowa University

Principal Investigator

ABE Etsuko  Showa Univ., School of Dentistry Associate Professor, 歯学部, 助教授 (70119147)

Co-Investigator(Kenkyū-buntansha) CHENG He Jin  Showa Univ., School of Dentistry Assistant, 歯学部, 助手 (10205049)
MIYAURA Chisato  Showa Univ., School of Dentistry Associate Professor, 歯学部, 構師 (20138382)
SHINKI Toshimasa  Showa Univ., School of Dentistry Associate Professor, 歯学部, 構師 (90138420)
SUDA Tatsuo  Showa Univ., School of Dentistry Professor, 歯学部, 教授 (90014034)
Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1992: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1991: ¥4,500,000 (Direct Cost: ¥4,500,000)
Keywordsosteoclast / osteoblast / complement / C3 receptor / 1alpha,25(OH)_2D_3 / C3 / bone resorption / 1α,25(OH)_2D_3 / マウス補体第3成分 / 骨髄間質細胞 / 肝臓 / 116kD / ILー1
Research Abstract

We found a 190 kDa protein produced by bone marrow-derived stromal cells (ST2) and primary osteoblastic cells in response to 1alpha,25(OH)_2D_3. The protein was purified and unequivocally identified as the third component of mouse complement (C3). It is well known that C3 protein in blood stream is derived preferentially from the liver. We therefore compared the regulatory mechanism of C3 protein and C3 gene expression by 1alpha,25(OH)_2D_3 in bone and liver. 1alpha,25(OH)_2D_3 greatly increased the C3 protein production and mRNA expression both dose- and time-dependently in primary osteoblastic cells. However, C3 production by hepatocytes occurred irrespective of the presence or absence of 1alpha,25-(OH)_2D_3. These results clearly indicate that 1alpha,25(OH)_2D_3 tissue-specifically regulates the synthesis of C3 in bone. Similar results were obtained in in vivo experiments using vitamin D-deficient mice. The mRNA level of C3 was undetectable in bone of vitamin D-deficient mice and became detectalbe as ealry as 24 h after 1alpha,25(OH)_2D_3 injection. In contrast, there was no significant difference in the expression of hepatic C3 mRNA between vitamin D-deficient and -supplemented mice.
Addition of antibodies of C3 and C3 receptors into mouse bone marrow cultures strikingly inhibited osteoclast formation induced by 1alpha,25(OH)_2D_3. The inhibitory effect of C3 antibody on osteoclast formation preferentially occurred when the antibody was added between days 3 and 4 (the commitment stage of osteoclast differentiation). These results suggest that the C3 produced by osteoblastic cells in response to 1alpha,25(OH)_2D_3 is somehow involved in osteoclast development, probably in the commitment of macrophage-like cells into preosteoclasts.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Sato,T.: "The specific production of the third component of complement by osteoblastic cells treated with 1α,25-dihydroxyvitamin D_3" FEBS Lett.285. 21-24 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Hong,M.H.: "Transcriptional regulation of the production of the third component of complement (C3) by 1α,25-dihydroxyvitamin D_3 in mouse marrow-derived stromal cells (ST2) and primary osteoblastic cells." Endocrinology. 129. 2774-2779 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Jin,C.H.: "1α,25-Dihydroxyvitamin D_3 regylates in vivo production of the third component of complenent (C3) in bone." Endocrinology. 131. 2468-2475 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 阿部 悦子: "活性型ビタミンDの刺激の下で骨芽細胞より産生される116kDの蛋白質" 日本骨代謝学会雑誌. 10. 205-211 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Toshiyuki Sato, et al.: "The specific production of the third component of complement by osteoblastic cells treated with 1alpha,25-dihydroxyvitamin D_3." FEBS Lett.285. 21-24 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Mei Hua Hong, et al.: "Transcriptional regulation of the production of the third component of complement (C3) by 1alpha,25-dihydroxyvitamin D_3 in mouse marrow-derived stromal cells (ST2) and primary osteoblastic cells." Endocrinology. 129. 2774-2779 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Cheng He Jin, et al.: "1alpha,25-Dihydroxyvitamin D_3 tissue-specifically regulates in vivo production of the third component of complement (C3) in bone." Endocrinology. 131. 2468-2475 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Sato,T.: "The specific production of the third component of complement by osteoblastic cells treated with 1α,25-dihydroxyvitamin D_3" FEBS Left.285. 21-24 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] Hong,M.H.: "Transcriptional regulation of the prodution of the third component of complement (C3) by 1α,25-dihydroxyvitamin D_3 in mouse marrow-derived stromal cells (ST2) and primary osteoblastic cells." Endocrinology. 129. 2774-2779 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] Jin,C.H.: "1α,25-Dihydroxyvitamin D_3 regulates in vivo production of the third componet of coplement (C3) in bone." Endocrinology. 131. 2468-2475 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 阿部 悦子: "活性型ビタミンDの刺激の下で骨芽細胞より産生される116kDの蛋白質" 日本骨代謝学会雑誌. 10. 205-211 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Sato,T.: "The specific production of the third component of complement by osteoblastic cells treated with 1α,25-dihydroxyvitamin D_3." FEBS Letters. 285. 21-24 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Hong,M.H.: "Transcriptional regulation of the production of the third component of complement (C3) by 1α,25-dihydroxyvitamin D_3in mouse marrow-derived stromal cells (ST2) and primary osteoblastic cells." Endocrinology. 129. 2774-2779 (1991)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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